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Investigation Of The Individual Soybean Root Nodule Microbiome, Parris Mica Mayhood
Investigation Of The Individual Soybean Root Nodule Microbiome, Parris Mica Mayhood
MSU Graduate Theses
Soybean is a valuable crop that establishes a symbiotic association with nitrogen-fixing microorganisms. Previous research suggests members of the genus Bradyrhizobium are the major nitrogen-fixing inhabitants of soybean root nodules. However, results from recent studies identified the presence of other bacteria, so called non-rhizobial endophytes (NREs), inside root nodules. All previous research has pooled nodules of different plants for bacterial community analysis, but this study investigated the presence and relative abundance of rhizobia and NREs within multiple root nodules of a single plant. Three sites were selected on Kendrick Farm in Springfield, MO, where three plants and rhizosphere soil were …
Characterizing Nodule Endophyte Communities In Glycine Max And Lablab Purpureus Using Next-Generation Sequencing, Scott David Mcelveen
Characterizing Nodule Endophyte Communities In Glycine Max And Lablab Purpureus Using Next-Generation Sequencing, Scott David Mcelveen
MSU Graduate Theses
Biological nitrogen fixation by rhizobia in the root nodules of legumes is a significant source of agricultural nitrogen in global crop production systems. The influence of and interaction of factors involved in nodule endophyte selection remain poorly understood. In the present study, the influences of crop rotation (soybean-legume vs. cotton-legume) and recalcitrant soil organic matter (compost amendment) on the relative distribution of endophytic bacteria in the root nodules of greenhouse-grown soybean and lablab were investigated by extracting, amplifying, and sequencing 16S ribosomal RNA (rRNA) and nifH genes. Neither preceding crop nor compost amendment were found to have an influence on …