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Cadmium Accumulation And Distribution In Lettuce And Barley, Fardausi Akhter Aug 2012

Cadmium Accumulation And Distribution In Lettuce And Barley, Fardausi Akhter

Electronic Thesis and Dissertation Repository

Cadmium (Cd) is a non-essential trace element and its environmental concentrations are increasing due to human activities. Edible plants can accumulate high concentrations of Cd, which could be toxic to humans. Understanding how and where Cd is stored in plants is important for ensuring lower concentration of Cd in the food. In this thesis, the accumulation and distribution of Cd in three agricultural plants, namely lettuce (Lactuca sativa L.), barley (Hordeum vulgare L.) and radish (Raphanus sativus L.), were investigated with a focus on the potential mechanisms involved in the localization of Cd in the root. The …


Protein Body Formation In Stable Transgenic Plants Of Nicotiana Tabacum Expressing Elastin-Like Polypeptide And Hydrophobin Fusion Proteins, Sonia P. Gutierrez Aug 2012

Protein Body Formation In Stable Transgenic Plants Of Nicotiana Tabacum Expressing Elastin-Like Polypeptide And Hydrophobin Fusion Proteins, Sonia P. Gutierrez

Electronic Thesis and Dissertation Repository

Plants are recognized as an efficient and inexpensive system to produce valuable recombinant proteins. However, the use of plants still faces two main limitations: the low accumulation levels of some recombinant proteins and the lack of efficient protein purification methods. Two fusion partners, elastin-like polypeptides (ELP) and hydrophobin I (HFBI) were found to increase the accumulation of recombinant proteins and induce the formation of protein bodies (PBs) when targeted to the ER in transient expression assays. In this study I examined the effect of these tags in stable transgenic plants of two Nicotiana tabacum cultivars when fused to green fluorescent …


Impacts Of Agricultural Disturbance On Communities Of Selected Soil Fungi (Agaricomycetes), Jessie R. Wong Aug 2012

Impacts Of Agricultural Disturbance On Communities Of Selected Soil Fungi (Agaricomycetes), Jessie R. Wong

Electronic Thesis and Dissertation Repository

The objective of this study was to use phylogeny-based and community-based analyses to compare the community composition of Agaricomycetes among four different agricultural treatments at the Kellogg Biological Station Long Term Ecological Research (KBS LTER) site. A phylogenetic tree that included 591 ribosomal DNA sequences previously obtained from KBS LTER documented the composition of Agaricomycete communities in each treatment. Sequences from KBS LTER were placed into 472 OTUs (putatively species-level operational taxonomic units defined by 99% or greater sequence similarity) and these were dominated by the Agaricales (with 330 OTUs), Cantharellales (39 OTUs), Hymenochaetales (29 OTUs), and Polyporales (23 OTUs). …


Hairy Roots As A Model To Investigate The Role Of Suberin In The Phytophthora Sojae-Soybean Pathosystem, Pooja Sharma Aug 2012

Hairy Roots As A Model To Investigate The Role Of Suberin In The Phytophthora Sojae-Soybean Pathosystem, Pooja Sharma

Electronic Thesis and Dissertation Repository

Part of the resistance mechanism of soybean (Glycine max [L.] Merr.) to Phytopthora sojae Kauf. & Gerd. involves pre-formed root suberin. In order to investigate the role of suberin in this host-pathogen interaction, I characterized hairy roots, formed as a result of Agrobacterium rhizogenes (Riker et al.) Conn infection, as a model to be used as a reliable soybean transformation system. I established hairy root cultures and demonstrated that they were a result of A. rhizogenes infection. The anatomy and suberin deposition in soybean hairy roots was examined, and found to be very similar to that of wild-type roots. …