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University of Tennessee, Knoxville

Food Science

Aichi virus

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Heat Inactivation Kinetics Of Tulane Virus And Aichi Virus, Sukriti Ailavadi May 2017

Heat Inactivation Kinetics Of Tulane Virus And Aichi Virus, Sukriti Ailavadi

Masters Theses

Human noroviruses (HNoVs) and Aichi virus (AiV) causes significant number of gastrointestinal diseases worldwide. Tulane virus (TV), a cultivable HNoV surrogate, is used to determine control measures against HNoV. The objectives of this study were to determine the heat inactivation kinetics of TV and AiV in cell-culture media and TV in spinach using the first-order and Weibull models. TV and AiV in cell-culture media at ~7 log PFU/ml in 2-ml glass vials were heated at 50-58°C [degree Celsius] up to 10 min in a circulating water-bath. Surviving infectious viruses were enumerated by standard plaque assays using confluent host cells in …


Blueberry Polyphenols As Natural Antimicrobial Agents Against Foodborne Viruses: Towards Understanding Their Mechanism And Applications In Food Systems, Snehal Shrikant Joshi Aug 2015

Blueberry Polyphenols As Natural Antimicrobial Agents Against Foodborne Viruses: Towards Understanding Their Mechanism And Applications In Food Systems, Snehal Shrikant Joshi

Doctoral Dissertations

Foodborne viruses are recognized as public health concerns worldwide and therefore effective strategies to control their spread are being researched. Blueberries are known for their health benefits and antimicrobial properties. This study aimed to (1) determine the antiviral effects of blueberry juice (BJ) and blueberry proanthocyanidins (B-PAC) against the infectivity of hepatitis A virus (HAV), Aichi virus (AiV) and human norovirus surrogates (feline calicivirus (FCV-F9) and murine norovirus (MNV-1)) at 37oC over 24-h using standard plaque assays; (2) evaluate antiviral effects in model foods (apple juice (AJ) and 2% milk) and simulated gastrointestinal conditions at 37oC; …


Control Of Emerging Foodborne Viruses Using Cranberry Extracts And Chemical Sanitizers, Snigdha Nitin Sewlikar Dec 2014

Control Of Emerging Foodborne Viruses Using Cranberry Extracts And Chemical Sanitizers, Snigdha Nitin Sewlikar

Masters Theses

Human norovirus (HNoV), hepatitis A virus (HAV) and Aichi virus (AiV) outbreaks that cause severe foodborne disease are on the rise globally. Strategies to prevent and mitigate the spread of these viral infections need to be investigated. HNoV, HAV and AiV are known to be resistant to most of the conventionally used chemical inactivation methods. Cranberry juice (CJ) and cranberry proanthocyanidins (C-PAC) have demonstrated antimicrobial and anti-inflammatory properties. CJ and C-PAC have been shown to exhibit antiviral activities against HNoV surrogates, feline calicivirus (FCV-F9) and murine norovirus (MNV-1) at room temperature. Hence, the objectives of this research were (1) To …