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Genomic And Transcriptomic Studies In Mycobacterium Avium Subspecies Paratuberculosis, J. P. Bannantine, Adel M. Talaat
Genomic And Transcriptomic Studies In Mycobacterium Avium Subspecies Paratuberculosis, J. P. Bannantine, Adel M. Talaat
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Microarray technology is an important tool in functional genomic research. It has enabled a deeper analysis of genomic diversity among bacteria belonging to the Mycobacterium avium complex (MAC). In addition, the expression of thousands of genes can be studied simultaneously in a single experiment. With the complete genome sequence of a bovine isolate of M. avium subspecies paratuberculosis, and the independent construction of DNA microarrays in our laboratories, transcriptomic studies for this veterinary pathogen are now possible. Furthermore, the bovine genome sequence project is completed and bovine arrays have been developed to examine host responses to infection with M. avium …
Threadfin Shad Impacts Phytoplankton And Zooplankton Community Structures In Channel Catfish Ponds, Bartholomew W. Green, Peter Perschbacher, Gerald Ludwig, Sara E. Duke
Threadfin Shad Impacts Phytoplankton And Zooplankton Community Structures In Channel Catfish Ponds, Bartholomew W. Green, Peter Perschbacher, Gerald Ludwig, Sara E. Duke
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Plankton community structure and chlorophyll a concentration were compared in 12 0.1-ha earthen ponds co-stocked with channel catfish (Ictalurus punctatus Rafinesque, 1818) in multiple-batch culture (initial biomass = 5,458 kg ha-1) and a planktivore, threadfin shad (Dorosoma petenense Güther, 1867; initial biomass = 449 kg ha-1), during the April-November growing season. We used a completely randomized design in 2 x 2 factorial arrangement to test planktivore level (presence or absence of threadfin shad) and channel catfish feeding frequency (daily or every 3rd d). Channel catfish were fed a 32% protein feed to apparent …
Century-Old Mystery Of Puccinia Striiformis Life History Solved With The Identification Of Berberis As An Alternate Host, Yue Jin, Les J. Szabo, Martin Carson
Century-Old Mystery Of Puccinia Striiformis Life History Solved With The Identification Of Berberis As An Alternate Host, Yue Jin, Les J. Szabo, Martin Carson
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
The life history of Puccinia striiformis remains a mystery because the alternate host has never been identified. Inoculation of grasses using aeciospores from naturally infected Berberis chinensis and B. koreana resulted in infection on Poa pratensis, producing uredinia typical of stripe rust caused by P. striiformis. Analyses using real-time polymerase chain reaction and DNA sequence confirmed the rust fungus as P. striiformis. Pycnia and aecia were produced on B. chinensis, B. holstii, B. koreana, and B. vulgaris after inoculation using germinating telia of P. striiformis f. sp. tritici. Wheat inoculated with aeciospores from B. chinensis resulted in …