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Predictive Promoter Activity In Soybean Using A Novel Cell-Suspension Culture And Transient Expression Analysis In Protoplasts, Mst Shamira Sultana
Predictive Promoter Activity In Soybean Using A Novel Cell-Suspension Culture And Transient Expression Analysis In Protoplasts, Mst Shamira Sultana
Masters Theses
Transient gene expression in plant protoplasts has been widely utilized for rapid functional screening of promoters. This project describes a novel soybean cell suspension culture and protoplast system for rapid and predictive analysis of promoter activity. Since recovery of transgenic lines is low in soybean stable transformation, high-throughput studies of promoter activity have intrinsic limitations. First, I describe a novel cell suspension culture system from leaf-derived callus. Once the cell culture is established, it is easy to maintain, sterile, and produces fast-growing cells amenable for protoplast production. Second, I describe an efficient and simple protoplast preparation procedure. The results revealed …
Breeding For Improved Oil Quality In High Yielding Soybean Lines Using Molecular Markers For Selecting Fad2-1a, Fad2-1b, Fad3a And Fad3c Mutant Alleles To Produce A High Oleic, Low Linolenic (Holl) Soybean., Alison Rose Willette
Masters Theses
Soybean oil is a major commodity in the US with practical uses for both food and industrial products. A declining market for soybean oil production caused by added restrictions on oil hydrogenation practices, has fueled improvements in oil industry standards. Typical soybean oil contains 8 to 12 g kg-1 of linolenic acid which causes unfavorable odor and rapid rancidity which has been dealt with using hydrogenation in the past. Scientists have identified fatty acid desaturase genes which when mutated, produce oil that does not require hydrogenation. Breeders use single nucleotide polymorphism (SNP) analysis to select for soybean mutations containing …