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Kinetics, Regulation And Purification Of L-Phenylalanine Ammonia-Lyase From Germinating Lettuce Seeds (Phenylpropanoids, Enzyme, Alpha-Aminoxy - Beta-Phenylpropionic Acid), George Jose Kudakasseril
Kinetics, Regulation And Purification Of L-Phenylalanine Ammonia-Lyase From Germinating Lettuce Seeds (Phenylpropanoids, Enzyme, Alpha-Aminoxy - Beta-Phenylpropionic Acid), George Jose Kudakasseril
Doctoral Dissertations
The kinetics of L-phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) in germinating lettuce (Lactuca sativa cv. Grand Rapids) seeds was investigated. The Km of the enzyme was determined to be 4.2 x 10('-5) M. PAL in lettuce seeds did not show tyrosine ammonia-lyase activity. The nature of inhibition of PAL by various substrate analogues and phenylpropanoid compounds was studied. Substrate analogues like D-phenylalanine, p-fluorophenylalanine, (beta)-phenyllactic acid and tryptophan were found to inhibit PAL competitively whereas tyrosine did not show any inhibition of enzyme activity. Of the phenylpropanoids used, cinnamic acid was found to be a competitive inhibitor whereas chlorogenic acid showed mixed …