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Biosynthesis And Metabolism Of Dolichol And Polyprenol In The Seeds And Seedlings Of Rye (Secale Cereale L), Robert Thomas Rymerson
Biosynthesis And Metabolism Of Dolichol And Polyprenol In The Seeds And Seedlings Of Rye (Secale Cereale L), Robert Thomas Rymerson
Digitized Theses
The seeds of monocotyledonous plants contain both dolichol, an homologous series of {dollar}\alpha{dollar}-saturated polyisoprenoid alcohols, and polyprenol, and homologous series of {dollar}\alpha{dollar}-unsaturated polyisoprenoid alcohols. Dolichyl phosphate (Dol-P) participates in the N-glycosylation of proteins. The function of polyprenol is unknown. Several possible roles for polyprenol were investigated in rye (Secale cereale L.). Methods for the extraction, separation, analysis, and quantification of polyisoprenoids were developed. Rye embryos contained twice as much polyprenol as dolichol; the ratio of polyprenyl phosphate to dolichyl phosphate was estimated at three to one.;Experiments on the metabolic labelling of polyisoprenoids with ({dollar}\sp3{dollar}H) H{dollar}\sb2{dollar}O, H{dollar}\sb3\lbrack\sp{lcub}32{rcub}{dollar}P) PO{dollar}\sb4{dollar}, or ({dollar}\sp{lcub}14{rcub}{dollar}C) mevalonolactone …
Physiological And Biochemical Significance Of Peanut (Arachis Hypogaea L) Peroxidases In Growth, Xiaohong Zheng
Physiological And Biochemical Significance Of Peanut (Arachis Hypogaea L) Peroxidases In Growth, Xiaohong Zheng
Digitized Theses
The molecular structures of peanut peroxidases from the cell suspension culture medium have been well characterized. The aim of present work is to investigate some of the physiological roles of these peroxidases, namely the functions in growth.;Specific polyclonal antibodies against both anionic and cationic peroxidases were produced by affinity chromatography. Immunological assays showed virtually no cross-reactivity between these antibodies and other compounds. The peroxidases from peanut cell suspension culture medium had {dollar}>{dollar}80% immunological homologies with those from cultured cells and hypocotyls as determined by Ouchterlony gel diffusion, western blot and competitive ELISA. Based on these homologies both the purified …