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Complex Systems Analysis In Selected Domains: Animal Biosecurity & Genetic Expression, Luke Trinity

Graduate College Dissertations and Theses

I first broadly define the study of complex systems, identifying language to describe and characterize mechanisms of such systems which is applicable across disciplines. An overview of methods is provided, including the description of a software development methodology which defines how a combination of computer science, statistics, and mathematics are applied to specified domains. This work describes strategies to facilitate timely completion of robust and adaptable projects which vary in complexity and scope. A biosecurity informatics pipeline is outlined, which is an abstraction useful in organizing the analysis of biological data from cells. This is followed by specific applications of …

Neurodevelopmental Roles Of Semaphorin6a/Plexina2 Signaling In Zebrafish, Sarah Elizabeth Emerson

Graduate College Dissertations and Theses

ABSTRACT

A multitude of complex cellular changes are required throughout development in order for a single cell to transform into a fully functioning organism. Cellular events including proliferation, migration, and differentiation have to be carefully controlled in order for development to proceed correctly. In order to study such dynamic processes, in vivo models are often utilized. Using the zebrafish (Danio rerio) as a model system, we have investigated the role of an axon guidance signaling pair, Semaphorin6A (Sema6A) and PlexinA2 (PlxnA2), in neurodevelopment.

A previous investigation into the developmental expression patterns of sema6A and plxnA2 in zebrafish, revealed overlapping expression …

The Sh2 Domain-Containing Adaptor Protein Shd Reversibly Binds The Crkl-Sh2 Domain And Knockdown Of Shdb Impairs Zebrafish Eye Development, Brendan Chandler

Graduate College Dissertations and Theses

The adaptor protein CT10-Regulator of Kinase (CRK) and the closely related CRK-Like (CRKL) are adaptor proteins that play important roles in many signaling pathways regulating cell proliferation and cell motility. A notable example is their required role in Reelin signaling during development of the laminated structures of the vertebrate central nervous system, including the cerebral cortex, cerebellum, hippocampus, and retina. As adaptors, CRK/CRKL are important in coupling phosphotyrosine signaling to G protein activity to regulate both cell proliferation and changes in the actin cytoskeleton, thereby exerting control over cell motility, and migration. While many proteins that interact with CRK/CRKL have …

Exercise In Developing Rats Promotes Plasticity In The Prefrontal Cortex: Behavioral And Neurobiological Indications, Meghan Eddy

Graduate College Dissertations and Theses

Physical exercise has repeatedly been shown to trigger positive effects on brain function including improved learning, memory, and executive functions. In addition, corresponding physiological changes have been observed, such as increased neurotrophic factors, changes in neurotransmitter concentrations, and increased dendritic spines. However, these changes have not been well described outside of the hippocampus, including the medial prefrontal cortex (mPFC), and have not been directly compared at different points of development. Because the prefrontal cortex is one of the last brain areas to fully mature, considering the age at which intervention, such as exercise, takes place is particularly important. Additionally, in …

Evolution Of Vernalization And Photoperiod-Regulated Genetic Networks In The Grass Subfamily Pooideae, Meghan Mckeown

Graduate College Dissertations and Theses

Flowering time is a carefully regulated trait that integrates cues from temperature and photoperiod to coordinate flowering at favorable times of the year. This dissertation aims to understand the evolution of genetic architecture that facilitated the transition of Pooideae, a subfamily of grass, from the tropics to the temperate northern hemisphere approximately 50 million years ago. Two traits hypothesized to have facilitated this evolutionary shift are the use of long-term low-temperature (vernalization) to ready plants for flowering, and long-day photoperiods to induce flowering. In chapter one I review literature on the regulation of grass flowering by vernalization and photoperiod, and …