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Rnai As A Tool For Understanding Germline Development In Caenorhabditis Elegans: Uses And Cautions, Eleanor M. Maine
Rnai As A Tool For Understanding Germline Development In Caenorhabditis Elegans: Uses And Cautions, Eleanor M. Maine
Biology - All Scholarship
RNA-mediated genetic interference (RNAi) has become a very useful tool for analyzing gene function in development and other processes. RNAi can be used as a complement to traditional genetic studies or as a primary means of determining biological function. However, the efficacy of RNAi depends on a variety of factors that the researcher must take into consideration. This review focuses on germline development in the nematode, Caenorhabditis elegans, and discusses the uses and limitations of RNAi in providing new information about gene function as well as the possible endogenous role RNAi plays in germline physiology.
Enhancers Of Gzp1, A Gene Required For Cell-Signaling In Caenorhabditis Elegans, Define A Set Of Genes Required For Germline Development, Li Qiao, James L. Lissemore, Pei Shu, Anne Smardon, Melanie B. Gelber, Eleanor M. Maine
Enhancers Of Gzp1, A Gene Required For Cell-Signaling In Caenorhabditis Elegans, Define A Set Of Genes Required For Germline Development, Li Qiao, James L. Lissemore, Pei Shu, Anne Smardon, Melanie B. Gelber, Eleanor M. Maine
Biology - All Scholarship
The distal tip cell (DTC) regulates the proliferation or differentiation choice in the Cmorhabditis ekgans germline by an inductive mechanism. Cell signaling requires a putative receptor in the germline, encoded by the glp-1 gene, and a putative signal from the DTC, encoded by the lag-2 gene. Both glp-1 and lag-2 belong to multigene gene families whose members are essential for cell signaling during development of various tissues in insects and vertebrates as well as C. elegans. Relatively little is known about how these pathways regulate cell fate choice. To identify additional genes involved in the glp-1 signaling pathway, we carried …
Intragenic Dominant Suppressors Of Glp-1, A Gene Essential For Cell-Signaling In Caenorhabditis Elegans, Support A Role For Cdcl O/Swz6/Ankyrin Motifs In Glp-1 Function, James L. Lissemore, Peter D. Currie, Christine M. Turk, Eleanor M. Maine
Intragenic Dominant Suppressors Of Glp-1, A Gene Essential For Cell-Signaling In Caenorhabditis Elegans, Support A Role For Cdcl O/Swz6/Ankyrin Motifs In Glp-1 Function, James L. Lissemore, Peter D. Currie, Christine M. Turk, Eleanor M. Maine
Biology - All Scholarship
The glp-1 gene product mediates cell-cell interactions required for cell fate specification during development in Caenorhabditis elegans. To identify genes that interact with glp-1, we screened for dominant suppressors of two temperature-sensitive glp-1 alleles and recovered 18 mutations that suppress both germline and embryonic glp-1 phenotypes. These dominant suppressors are tightly linked to glp-1 and do not bypass the requirement for a distal tip cell, which is thought to be the source of a signal that is received and transduced by the GLP-1 protein. Using single-strand conformation polymorphism (SSCP) analysis and DNA sequencing, we found that at least 17 suppressors …