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A Preliminary Investigation Into The Effects Of Lucilia Sericata (Meigen) Blow Fly Larval Feeding On Sharp Force Trauma (Sft) Wound Patterns In Decomposing Bones Of Sus Scrofa Domesticus, Erica L. Klafehn Dec 2021

A Preliminary Investigation Into The Effects Of Lucilia Sericata (Meigen) Blow Fly Larval Feeding On Sharp Force Trauma (Sft) Wound Patterns In Decomposing Bones Of Sus Scrofa Domesticus, Erica L. Klafehn

Student Theses

An important aspect of forensic investigations involves the characterization and analysis of bone related trauma as this can provide valuable information regarding the manner and circumstances of death. Bone trauma can be classified into three main categories, which include Sharp Force Trauma (SFT), Blunt Force Trauma (BFT), and Gunshot Trauma. Previous experiments have explored SFT on flesh and bones, specifically made with knives or saws, coupled with various microscopic analytical methods. Minimal research has been done integrating both a forensic entomology and forensic anthropology approach, so it is imperative to understand previous literature detailing the behaviors of insects (blow flies), …


Wave Optics In Scanning Electron Microscopy, Surya Kamal Sep 2021

Wave Optics In Scanning Electron Microscopy, Surya Kamal

Theses

Better quality imaging is often needed for all optical instruments including the scanning electron microscope (SEM). Inside the SEM, an electron beam is generated by applying a voltage to the electron gun. The electron beam propagates through the SEM column and illuminates the specimen with a small probe. Our research is oriented towards the end goal of obtaining better images by modeling the SEM column which contains the lens system needed for probe formation. This probe which scans the specimen to produce the final image is one of the pivotal factors which determines the fundamental resolution of a SEM. We …


Evaluation Of The Potential Of Automated Sem-Eds Analysis For The Discrimination Of Inorganic Soil Particles, Anna S. Duggar Sep 2021

Evaluation Of The Potential Of Automated Sem-Eds Analysis For The Discrimination Of Inorganic Soil Particles, Anna S. Duggar

Dissertations, Theses, and Capstone Projects

Soil, that complex mixture of minerals, organic particles, chemicals, and anthropogenic materials, is ubiquitous and easily transferred, which gives it forensic relevance. Forensic soil analysis has traditionally included the identification and comparison of both organic and inorganic components, the inorganic portion being most commonly examined by light microscopy and other instrumental techniques. Predominantly, the inorganic materials in soil are naturally-occurring minerals, contributed as grains from the surrounding bedrock, which gives a soil its regional character. That parent material then undergoes physical, chemical, and anthropogenic changes producing wide geographic variation within a single region. This variation forms the basis for potential …


Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction And Attachment Of Primary Human Dental Pulp Stem Cells In Real-Time Ex Vivo, Yeon Jee Yoo, Min Ji Kang, Hiran Perinpanayagam, Joo Cheol Park, Seung Ho Baek, Kee Yeon Kum Aug 2021

Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction And Attachment Of Primary Human Dental Pulp Stem Cells In Real-Time Ex Vivo, Yeon Jee Yoo, Min Ji Kang, Hiran Perinpanayagam, Joo Cheol Park, Seung Ho Baek, Kee Yeon Kum

Dentistry Publications

This study investigated if non-thermal atmospheric pressure plasma (NTAPP) treatment of root dentin surfaces promotes human dental pulp stem cell (hDPSCs) adhesion. Freshly extracted human single-rooted teeth (n = 36) were decoronated and cut (first vertically, then horizontally) into root dentin slices (3 mm thick). Primary hDPSCs cultures were seeded onto slices randomly assigned to pretreatment groups (n = 9/group): NaOCl (1.5%), EDTA (17%) then NTAPP (Group I); NaOCl then NTAPP (Group II); NaOCl then EDTA (Group III); and NaOCl alone (Group IV). Cell viability and proliferation were measured using MTT assay with log-linear statistical analysis. Cell attachment and spreading …


Using Quantitative Stereology On High Resolution Sem Images To Estimate Diatom Percentages, Ariadna Covarrubias Ornelas Jul 2021

Using Quantitative Stereology On High Resolution Sem Images To Estimate Diatom Percentages, Ariadna Covarrubias Ornelas

Dissertations and Theses

Diatoms are single-celled organisms of various shapes and sizes typically found in aquatic environments. When diatoms die, the organic material decomposes, and the outer skeletons (i.e., frustules) settle and accumulate as sedimentary deposits. These soils, called diatomaceous soils, exhibit nontraditional behavior since the diatom particles are typically hollow skeletons composed of amorphous silica with intricately patterned and abrasive surfaces. Recent studies have shown that diatomaceous soils are challenging geomaterials since even a small percentage of diatom particles will notably affect engineering behavior. Furthermore, laboratory studies on diatomaceous soil mixtures have demonstrated that many engineering soil properties depend on the percentage …


Atomic Layer Deposition Of Sodium Fluoride Thin Films, Sara Kuraitis, Donghyeon Kang, Anil U. Mane, Hua Zhou, Jake Soares, Jeffrey W. Elam, Elton Graugnard May 2021

Atomic Layer Deposition Of Sodium Fluoride Thin Films, Sara Kuraitis, Donghyeon Kang, Anil U. Mane, Hua Zhou, Jake Soares, Jeffrey W. Elam, Elton Graugnard

Materials Science and Engineering Faculty Publications and Presentations

The need for advanced energy conversion and storage devices remains a critical challenge amid the growing worldwide demand for renewable energy. Metal fluoride thin films are of great interest for applications in lithium-ion and emerging rechargeable battery technologies, particularly for enhancing the stability of the electrode-electrolyte interface and thereby extending battery cyclability and lifetime. Reported within, sodium fluoride (NaF) thin films were synthesized via atomic layer deposition (ALD). NaF growth experiments were carried out at reactor temperatures between 175 and 250 °C using sodium tert-butoxide and HF-pyridine solution. The optimal deposition temperature range was 175–200 °C, and the resulting …


Achene Micromorphology Of The Genus Potentilla L. (Rosaceae) In Iran And Its Systematicapplication, Samira Sadeghi, Marzieh Beygom Faghir, Farideh Attar, Ali Alaami Jan 2021

Achene Micromorphology Of The Genus Potentilla L. (Rosaceae) In Iran And Its Systematicapplication, Samira Sadeghi, Marzieh Beygom Faghir, Farideh Attar, Ali Alaami

Turkish Journal of Botany

Achene micromorphological evidence belonging to 32 species of Potentilla from Iran were studied using scanning electron microscopy (SEM). Multivariate analysis was used to estimate the potential contribution of the quantitative and qualitative traits to the species relationships. Based on the present results, the achene micromorphological traits exhibited considerable variations. The SEM observations revealed 4 types of achene shapes, 4 main types and 6 sub types of surface patterns, 3 types of wax, 8 types, 15 subtypes, and 4 categories of sculpturing, 2 types of anticlinal cell boundaries, and 3 types of outer periclinal walls. In addition, the results demonstrated variations …


Assessment Of Erythrocyte Morphology In Patients With Type 2 Diabetes Mellitus: A Pilotstudy Of Electron Microscopy-Based Analysis In Relation To Healthy Controls, Tülay Mortaş, Şenay Arikan Durmaz, Şaban Cem Sezen, Yasemi̇n Savranlar Jan 2021

Assessment Of Erythrocyte Morphology In Patients With Type 2 Diabetes Mellitus: A Pilotstudy Of Electron Microscopy-Based Analysis In Relation To Healthy Controls, Tülay Mortaş, Şenay Arikan Durmaz, Şaban Cem Sezen, Yasemi̇n Savranlar

Turkish Journal of Medical Sciences

Background/aim: The present study aimed to assess erythrocyte morphology in newly diagnosed type 2 diabetes mellitus patients using scanning electron microscopy. Materials and methods: In total, 30 patients admitted to endocrine outpatient clinics were included in the study. The patients were divided into two groups according to their fasting blood glucose levels: type 2 diabetes mellitus (n = 15, fasting blood glucose levels ≥ 126 mg/dL) and control (n = 15, fasting blood glucose levels < 99 mg/dL). The patient's demographic characteristics, haemoglobin A1c levels, and scanning electron microscopy findings regarding erythrocyte morphology were recorded. Results: There was no significant difference between the control and type 2 diabetes mellitus group in terms of the participants' age (51.13 ± 8.53 vs. 50.33 ± 8.72 years, p = 0.8) and the male/female ratio (9/6 vs. 9/6). In the control group, discocytes were abundant, echinocytes were rare, and spherocytes were absent. On the other hand, discocytes were less common and echinocyte-shaped erythrocytes were more common in the type 2 diabetes mellitus group than in the control group. In addition, spherocytes were detected in the type 2 diabetes mellitus group. Moreover, the diameter of discocytes was significantly lower (p = 0.014), and blood glucose and haemoglobin A1c levels were significantly higher (p < 0.05 for both) in the type 2 diabetes mellitus group than in the control group. Conclusion: Our findings indicate that high glucose levels in type 2 diabetes mellitus patients lead to significant alterations in erythrocyte morphology, including decreased erythrocyte deformability and the formation of echinocytes and spherocytes due to eryptosis. The possibility of decreased erythrocyte deformability due to excessive eryptosis may disturb microcirculation in newly diagnosed, treatment-naïve type 2 diabetes mellitus patients who do not have any complications.