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The Co-Delivery Of Syngeneic Adipose-Derived Stromal Cells And Macrophages On Decellularized Adipose Tissue Bioscaffolds For In Vivo Soft Tissue Regeneration, Hisham A. Kamoun

Electronic Thesis and Dissertation Repository

Decellularized adipose tissue (DAT) bioscaffolds are a promising platform for the delivery of pro-regenerative cell populations with the goal of promoting adipose tissue regeneration. The current study investigated the effects of seeding DAT bioscaffolds with syngeneic bone marrow-derived macrophages and/or adipose-derived stromal cells (ASCs) on in vivo soft tissue regeneration. Methods were established to derive the macrophages from MacGreen mice, which were dynamically seeded onto the DAT scaffolds alone or in combination with ASCs. Seeded and unseeded scaffolds were implanted subcutaneously into C57Bl/6 mice. At 2 and 4 weeks, cell infiltration, angiogenesis, and adipogenesis were analyzed through histology and immunohistochemistry. …

Preparation And Characterization Of Collagen-Hydroxyapatite-Based Composites For Biomedical Applications, Le Yu

Doctoral Dissertations

Collagen, the most abundant protein, and hydroxyapatite, the main component in natural bone, are usually used as a biomimetic composite in many biomedical applications due to their excellent biocompatibility, great biodegradability, and flexible structural and compositional alterability. In this dissertation, a systematic study was conducted to design, develop and characterize collagen-hydroxyapatite-based (Col-HA-based) composite materials and explore their wide biomedical applications.

First, the biomeralization mechanism of collagen was investigated. The degree of intrafibrillar mineralization of Col-HA fibrils was enhanced by the employment of carboxyl-rich brushlike polymers, which is believed to be capable of replicating the sequestration functions of non-collagenous proteins (NCPs) …

Biomaterial Substrate Modifications That Influence Cell-Material Interactions To Prime Cellular Responses To Nonviral Gene Delivery, Amy Mantz, Angela K. Pannier

Department of Biological Systems Engineering: Papers and Publications

Gene delivery is the transfer of exogenous genetic material into somatic cells to modify their gene expression, with applications including tissue engineering, regenerative medicine, sensors and diagnostics, and gene therapy. Viral vectors are considered the most effective system to deliver nucleic acids, yet safety concerns and many other disadvantages have resulted in investigations into an alternative option, i.e. nonviral gene delivery. Chemical nonviral gene delivery is typically accomplished by electrostatically complexing cationic lipids or polymers with negatively charged nucleic acids. Unfortunately, nonviral gene delivery suffers from low efficiency due to barriers that impede transfection success, including intracellular processes such as …

Evaluating The Bioactivity And Biocompatibility Of Nylon Coated Ultra-High Molecular Weight Polyethylene (Uhmwpe), Nancy Hassanein

Theses and Dissertations

Ultra-high molecular weight polyethylene (UHMWPE) semi crystalline biomaterial is one of the gold standard materials that are used as a bearing surface in total joint replacement surgeries. However, wear particles generated by UHMWPE due to the relative motion between the different components of the bearing, would eventually result in osteolysis and implant failure. For this, many attempts to enhance the properties of UHMWPE were done, including coating the UHMWPE with nylon 6,6 which was reported to improve its mechanical properties and biocompatibility. In this study, the antibacterial activity, moisture and SBF absorption, pH effect, bioactivity, biocompatibility and wound healing ability …

Fibrous Bone Tissue Engineering Scaffolds Prepared By Wet Spinning Of Plga, Nergi̇s Abay Akar, Görke Gürel Peközer, Gamze Köse

Turkish Journal of Biology

Having a self-healing capacity, bone is very well known to regenerate itself without leaving a scar. However, critical size defects due to trauma, tumor, disease, or infection involve bone graft surgeries in which complication rate is relatively at high levels. Bone tissue engineering appears as an alternative for grafting. Fibrous scaffolds are useful in tissue engineering applications since they have a high surface-to-volume ratio, and adjustable, highly interconnected porosity to enhance cell adhesion, survival, migration, and proliferation. They can be produced in a wide variety of fiber sizes and organizations. Wet spinning is a convenient way to produce fibrous scaffolds …

Biomaterials For Bone Regeneration, Youqu Shen

Doctoral Dissertations

"The purpose of this Ph.D. research is to investigate and improve two classes of hydroxyapatite (HA)-based biomaterials for bone repair: calcium phosphate microspheres and bioactive silicate glass scaffolds. These biomaterials were prepared with modified compositions and microstructures and then were evaluated for bone regeneration.

The open HA microspheres with dense convex surfaces and rough and porous concave surfaces were obtained by sectioning closed hollow HA microspheres. Bone regeneration with the open HA microspheres was greater than with the closed HA microsphere at 12 weeks. Hollow biphasic calcium phosphate (BCP) microspheres have been prepared with different fractions of HA and β-TCP …