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Total Polycyclic Aromatic Hydrocarbon (Tpah) Results: Chemically Enhanced Water-Accommodated Fractions (Cewaf)-Exposed Florida Pompano F1 Generation, Dana Wetzel, Tracy Sherwood, Rebecca Medvecky, Christelle Miller
Total Polycyclic Aromatic Hydrocarbon (Tpah) Results: Chemically Enhanced Water-Accommodated Fractions (Cewaf)-Exposed Florida Pompano F1 Generation, Dana Wetzel, Tracy Sherwood, Rebecca Medvecky, Christelle Miller
C-IMAGE data
This dataset contains total polycyclic aromatic hydrocarbon (TPAH) concentrations in exposure seawater solutions and in livers of laboratory-raised Florida pompano exposed to south Louisiana crude oil.
Use Of Spectrofluorometry To Detect Petroleum Hydrocarbons In The Marine Environment, Mary Iris Abercrombie
Use Of Spectrofluorometry To Detect Petroleum Hydrocarbons In The Marine Environment, Mary Iris Abercrombie
USF Tampa Graduate Theses and Dissertations
The genesis of this research was the Deepwater Horizon oil spill, which discharged petroleum and gas into the Gulf of Mexico for 87 days in 2010. High-resolution fluorescence spectroscopy was employed for the detection of petroleum in seawater samples following the oil spill. Fluorescence arises from the chemical structure of π-bonding in C=C bonds, especially those in aromatic structures. Spectrofluorometry was also used to observe and track the formation of petroleum plumes in seawater undergoing controlled physical dispersion in a wave tank, both with and without the addition of chemical dispersant. Further, the changing fluorescence characteristics of a broad range …
Improving The Integration Of Restoration And Conservation In Marine And Coastal Ecosystems: Lessons From The Deepwater Horizon Disaster, Richard L. Wallace, Gilbert Gilbert, John E. Reynolds Iii
Improving The Integration Of Restoration And Conservation In Marine And Coastal Ecosystems: Lessons From The Deepwater Horizon Disaster, Richard L. Wallace, Gilbert Gilbert, John E. Reynolds Iii
C-IMAGE Publications
In the wake of the Deepwater Horizon disaster, much has been learned about the biological, ecological, physical, and chemical conditions of the Gulf of Mexico. In parallel, the research community has also gained insight about the social and organizational structures and processes necessary for oil spill response and subsequent marine and coastal restoration. However, even with these lessons from both the Deepwater Horizon and previous spills, including 1989’s Exxon Valdez and the Ixtoc 1 in 1979, our understanding of how to avoid future crises has not advanced at the same pace as offshore oil and gas development. We argue that …
Three-Dimensional Daily Hindcast Of Oil Concentrations And Oil Mass Estimates From The Far-Field Modeling Of A Deep Water Spill Scenario In The Northwest African Continental Shelf, Using The Connectivity Modeling System On A Probabilistic Approach, Claire B. Paris, Natalie Perlin, Ana C. Vaz
Three-Dimensional Daily Hindcast Of Oil Concentrations And Oil Mass Estimates From The Far-Field Modeling Of A Deep Water Spill Scenario In The Northwest African Continental Shelf, Using The Connectivity Modeling System On A Probabilistic Approach, Claire B. Paris, Natalie Perlin, Ana C. Vaz
C-IMAGE data
The dataset contains the numerical results of probabilistic forecasts for possible oil spills in the Northwest African continental Shelf, around Senegal. The origin of possible oil spills scenarios are deep wells proposed to operate in and around Cayar Canyon (Senegal). Oil dispersal and concentrations were simulated using the latest updated version of the oil application of the Connectivity Modeling System (CMS) or oil- CMS. In this version, the specified hydrocarbon pseudo-components are in the same droplet. The post-processing analysis yielded 4-D spatiotemporal data of the oil concentrations and oil mass on a regular horizontal and vertical grid, as well as …
Field Collected Red Fish And Southern Flounder: Tpah Tissue Results, Dana Wetzel
Field Collected Red Fish And Southern Flounder: Tpah Tissue Results, Dana Wetzel
C-IMAGE data
This dataset contains total polycyclic aromatic hydrocarbon (TPAH) concentrations of south Louisiana crude oil in sediment and tissue of field-collected red drum (Sciaenops ocellatus) and southern flounder (Paralichthys lethostigma) in the Gulf of Mexico from 2018-08-13 to 2018-08-16.
Associations Between Chronic Exposure To Polycyclic Aromatic Hydrocarbons And Health Indices In Gulf Of Mexico Tilefish (Lopholatilus Chamaeleonticeps) Post Deepwater Horizon, Susan M. Syder, Erin Pulster, Steven Murawski
Associations Between Chronic Exposure To Polycyclic Aromatic Hydrocarbons And Health Indices In Gulf Of Mexico Tilefish (Lopholatilus Chamaeleonticeps) Post Deepwater Horizon, Susan M. Syder, Erin Pulster, Steven Murawski
C-IMAGE Publications
A time series of polycyclic aromatic hydrocarbon (PAH) data was collected for Gulf of Mexico demersal fishes in the years following the Deepwater Horizon oil spill (2012–2017). Tilefish (Lopholatilus chamaeleonticeps ) were sampled via demersal longline at repeat stations in the northern Gulf of Mexico in 2012 to 2015 and 2017. Bile samples (n = 256) were analyzed via high‐performance liquid chromatography with fluorescence detection for PAH metabolites as a biomarker of exposure to PAHs. Liver tissues (n = 230) were analyzed for accumulation of PAHs and alkylated homologs via quick, easy, cheap, effective, rugged, and safe …
Muscle Concentrations Of Polycyclic Aromatic Hydrocarbons (Pahs) In Red Snapper And Yellowedge Grouper Data Collected Aboard R/V Weatherbird Ii Cruises Wb-0915 And Wb-0816 In The Southern Gulf Of Mexico From 2015-09-16 To 2016-08-19, Adolfo Gracia
C-IMAGE data
This dataset contains muscle concentrations of Polycyclic Aromatic Hydrocarbons (PAHs) in Red Snapper and Yellowedge Grouper data collected aboard R/V Weatherbird II cruises WB-0915 and WB-0816 in the southern Gulf of Mexico from 2015-09-16 to 2016-08-19. Red snapper and Yellowedge grouper were caught in Campeche Bay using demersal longline sampling gear. Longline sets were generally deployed at six sites along pre-defined transects from relatively shallow to deep continental shelf areas. The nominal depths sampled along each transect were 37, 73, 110, 146, 183 and 274 m. At each station, eight km of 544 kg test monofilament main line was deployed, …
Liver Anomalies In Red Snapper (Lutjanus Campechanus) Collected Aboard R/V Weatherbird Ii Cruises Wb-1701 And Wb-1603 In The Southern Gulf Of Mexico From 2015-09-17 To 2016-08-19, Adolfo Gracia
C-IMAGE data
This dataset contains liver anomalies in red snapper (Lutjanus campechanus) that were collected aboard R/V Weatherbird II cruises WB-1701 and WB-1603 in the southern Gulf of Mexico from 2015-09-17 to 2016-08-19. Red snapper samples were caught in Campeche Bay and Yucatan Shelf using demersal longline sampling gear. At each station, 544 kg test monofilament main line was deployed, with a mean of 433 baited hooks per set. Livers of red snappers were examined for hepatic parenchymal changes. The dataset includes the date, location of sample collection sites, the common and scientific name of the fish examined, the classification and the …
Input, Output And Complete Run Files For The Atlantis Ecosystem Model Deepwater Horizon Oil Spill Simulations Including Fish And Invertebrate Effects, Cameron Ainsworth, Lindsey N. Dornberger
Input, Output And Complete Run Files For The Atlantis Ecosystem Model Deepwater Horizon Oil Spill Simulations Including Fish And Invertebrate Effects, Cameron Ainsworth, Lindsey N. Dornberger
C-IMAGE data
These data include input and output files from the Gulf of Mexico Atlantis model. The simulations are published in Dornberger et al., 2019. The dataset includes every file needed to recreate the simulations: 1) the 18 spatial forcing files needed to recreate the sensitivity analysis; these vary the oil sensitivity threshold K, the fish oil threshold and the invertebrate oil threshold. The file naming scheme indicates parameter values used. 2) the three .prm files needed to recreate the different levels of fishing mortality (F=0.5, 1, 2.0, 10), The F rate indicates an increase in fishing mortality across all fishing sectors …
Concentrations Of Polycyclic Aromatic Hydrocarbons (Pahs) In Liver Tissue Of Golden Tilefish Collected Aboard Multiple R/V Weatherbird Ii Cruises In The Gulf Of Mexico From 2012-08-13 To 2017-08-01, Erin Pulster, S. M. Synder, Steven A. Murawski
Concentrations Of Polycyclic Aromatic Hydrocarbons (Pahs) In Liver Tissue Of Golden Tilefish Collected Aboard Multiple R/V Weatherbird Ii Cruises In The Gulf Of Mexico From 2012-08-13 To 2017-08-01, Erin Pulster, S. M. Synder, Steven A. Murawski
C-IMAGE data
This dataset contains the concentrations of polycyclic aromatic hydrocarbons (PAHs) in liver tissue of golden tilefish (Lopholatilus chamaeleonticeps) collected aboard multiple R/V Weatherbird II cruises WB1304 (2012-08-13 to 2012-08-25), WB1403 (2013-08-15 to 2013-08-30), WB1502 (2014-08-05 to 2014-08-20), WB1602 (2015-08-15 to 2015-08-30), WB1603 (2015-09-10 to 2015-10-02), WB1701 (2016-08-02 to 2016-09-09), and WB1736 (2017-07-18 to 2017-08-01) in the Gulf of Mexico during the Gulf of Mexico-wide surveys from 2012-2017. PAH was extracted from livers with a modified QuEChERS (Bond Elut, Agilent Technologies, Santa Clara, CA, USA) method optimized specifically for this study species. Extracts were analyzed using Agilent’s 7890B gas chromatograph (GC) …
Oil Degradation In Laboratory Simulated Shallow Sandy Sediments From Pensacola Beach, Florida, Will Overholt
Oil Degradation In Laboratory Simulated Shallow Sandy Sediments From Pensacola Beach, Florida, Will Overholt
C-IMAGE data
This dataset contains the results of laboratory experiments performed on samples of shallow sandy sediments from Pensacola Beach, Florida with the goal of determining how beach microbes will respond to Macondo oil buried in sandy sediments. The dataset includes information about oxygen profiles, nitrate concentrations, nitrite concentrations, ammonium concentrations, phosphate concentrations, microbial 16S amplicons, microbial metagenomes, and total petroleum hydrocarbon concentrations.
Hepatic Polycyclic Aromatic Hydrocarbons (Pahs) In Gulf And Southern Hake Collected Aboard Multiple R/V Weatherbird Ii Cruises In The Gulf Of Mexico From 2012-08-21 To 2015-09-24, Erin Pulster, Steven A. Murawski, Rachel Struch
Hepatic Polycyclic Aromatic Hydrocarbons (Pahs) In Gulf And Southern Hake Collected Aboard Multiple R/V Weatherbird Ii Cruises In The Gulf Of Mexico From 2012-08-21 To 2015-09-24, Erin Pulster, Steven A. Murawski, Rachel Struch
C-IMAGE data
This dataset contains hepatic polycyclic aromatic hydrocarbons (PAHs) in Gulf and Southern Hake collected aboard multiple R/V Weatherbird II cruises in the Gulf of Mexico from 2012-08-21 to 2015-09-24. PAH was extracted from liver samples using a modified QuEChERS (Bond Elut, Agilent Technologies, Santa Clara, CA, USA) method optimized specifically for these study species. Extracts were analyzed using Agilent’s 7890B gas chromatograph (GC) coupled to a 7010 tandem mass spectrometer (MS/MS) operating in multiple reactions mode (MRM). The dataset contains the location, date, biometrics, and the total concentration of polycyclic aromatic hydrocarbon (PAHs) in livers. The cruise documentation was provided …
Sub-Adult Southern Flounder Exposure To Crude Oil-Contaminated Sediment: Oxidative Stress Assays & Dna Damage Assays, Dana Wetzel, Tracy Sherwood, Christelle Miller, Rebecca Medvecky
Sub-Adult Southern Flounder Exposure To Crude Oil-Contaminated Sediment: Oxidative Stress Assays & Dna Damage Assays, Dana Wetzel, Tracy Sherwood, Christelle Miller, Rebecca Medvecky
C-IMAGE data
In this experiment, fish (southern flounder) were exposed to oiled (Louisiana crude oil) sediments in a mesocosm experiment for 30 days. At the end of this period, the fish were examined for oxidative stress and DNA damage. The dataset contains measurements of glutathione (GSH), malondialdehye assay (MDA), AP (apurinic or apyrimidinic) sites, percent cell viability as well as basic details of fish such as gender, length and weight.
Rna-Seq Transcriptome Characterization Of Tissue From Sub-Adult Southern Flounder Exposed To Louisiana Crude Oil Contaminated Sediment, Dana Wetzel, Tracy Sherwood, Rebecca Medvecky
Rna-Seq Transcriptome Characterization Of Tissue From Sub-Adult Southern Flounder Exposed To Louisiana Crude Oil Contaminated Sediment, Dana Wetzel, Tracy Sherwood, Rebecca Medvecky
C-IMAGE data
Illumina high-throughput RNA-sequencing technology was used to sequence, assemble and annotate the transcriptome of tissues from southern flounder exposed to Louisiana crude oil. This dataset contains SRA accession numbers for RNA sequencing data located at the National Center for Biotechnology Information, log2 fold change, annotated assembly, and fasta files.
Sub-Adult Southern Flounder Exposure To Crude Oil-Contaminated Sediment: Immune Function Assays & Gene Expression, Dana Wetzel
Sub-Adult Southern Flounder Exposure To Crude Oil-Contaminated Sediment: Immune Function Assays & Gene Expression, Dana Wetzel
C-IMAGE data
Controlled oil exposure study to evaluate the effects of south Louisiana crude oil, via contaminated sediment, on fish health. Cytokine concentrations were analyzed in southern flounder after exposure to oil-contaminated sediment for 30 days. This dataset contains Ct and percent efficiencies of deferentially expressed genes of southern flounder exposed to sediments contaminated with south Louisiana crude oil.
Red Drum Diet Exposure To Deepwater Horizon Crude Oil Contaminated Feed: Oxidative Stress Assays, Dl Wetzel, Ta Sherwood, Ca Miller, Rl Medvecky
Red Drum Diet Exposure To Deepwater Horizon Crude Oil Contaminated Feed: Oxidative Stress Assays, Dl Wetzel, Ta Sherwood, Ca Miller, Rl Medvecky
C-IMAGE data
Changes in the ratio of reduced glutathione and oxidized glutathione and total antioxidant power of red drum fed a diet of pellets saturated with south Louisiana crude oil.
Cewaf-Exposed Florida Pompano Reproduction: Oxidative Stress And Dna Damage Assays, Dl Wetzel, Ta Sherwood, Ca Miller, Rl Medvecky
Cewaf-Exposed Florida Pompano Reproduction: Oxidative Stress And Dna Damage Assays, Dl Wetzel, Ta Sherwood, Ca Miller, Rl Medvecky
C-IMAGE data
Analysis of the oxidative stress and DNA damage in adult Florida pompano after reproduction and exposure to chemically enhanced water accommodated fraction (CEWAF) of south Louisiana crude oil.
Cewaf-Exposed Florida Pompano Reproduction: Tpah Results, Dl Wetzel, Ta Sherwood, Ca Miller, Rl Medvecky
Cewaf-Exposed Florida Pompano Reproduction: Tpah Results, Dl Wetzel, Ta Sherwood, Ca Miller, Rl Medvecky
C-IMAGE data
Controlled oil exposure study to evaluate the effects of south Louisiana crude oil chemically enhanced water accommodated fraction (CEWAF) exposure on Florida pompano and reproduction. Data include total polycyclic aromatic hydrocarbon (tPAH) results for exposure solutions, pompano livers, and pompano whole bodies, as well as volatile organic compound (VOC) results for exposure solutions.
Cewaf Water Exposed Florida Pompano F0 Reproduction: Lipid Class Composition, Dl Wetzel, Ta Sherwood, Ca Miller, Rl Medvecky
Cewaf Water Exposed Florida Pompano F0 Reproduction: Lipid Class Composition, Dl Wetzel, Ta Sherwood, Ca Miller, Rl Medvecky
C-IMAGE data
Lipid class composition by Iatroscan of adult Florida pompano exposed to south Louisiana crude oil via CEWAF-spiked water.
Total Petroleum Hydrocarbon Results From Sub-Adult Red Drum Exposure To Chemically Enhanced Water Accommodated Fraction For 4 Days Uptake, 6 Days Duration, Dana Wetzel, Tracy Sherwood, Rebecca Medvecky, Christelle Miller
Total Petroleum Hydrocarbon Results From Sub-Adult Red Drum Exposure To Chemically Enhanced Water Accommodated Fraction For 4 Days Uptake, 6 Days Duration, Dana Wetzel, Tracy Sherwood, Rebecca Medvecky, Christelle Miller
C-IMAGE data
Controlled oil exposure study to evaluate the effects of south Louisiana crude oil, via chemically enhanced water accommodated fraction (CEWAF), on red drum health. Data include exposure solution total polycyclic aromatic hydrocarbon (tPAH) results, red drum liver tPAH results, red drum whole body tPAH results, and exposure solution volatile organic compounds (VOCs).
Sub-Adult Southern Flounder Exposure To Crude Oil-Contaminated Sediment: Lipid Composition And Fatty Acids, Dl Wetzel, Ta Sherwood, Ca Miller, Rl Medvecky
Sub-Adult Southern Flounder Exposure To Crude Oil-Contaminated Sediment: Lipid Composition And Fatty Acids, Dl Wetzel, Ta Sherwood, Ca Miller, Rl Medvecky
C-IMAGE data
Controlled oil exposure study to evaluate the effects of south Louisiana crude oil, via contaminated sediment, on fish health. Data include lipid class composition using Iatroscan of southern flounder exposed via sediment contaminated with south Louisiana crude oil.
Red Drum Cewaf Uptake/Depuration Study: Oxidative Stress Assays & Dna Damage Assays, Dl Wetzel, Ta Sherwood, Rl Medvecky
Red Drum Cewaf Uptake/Depuration Study: Oxidative Stress Assays & Dna Damage Assays, Dl Wetzel, Ta Sherwood, Rl Medvecky
C-IMAGE data
Analysis of the oxidative stress markers in red drum after exposure to CEWAF of south Louisiana crude oil. Analysis of DNA damage in red drum after exposure to CEWAF of south Louisiana crude oil.
Red Drum Cewaf Uptake/Depuration Study: Lipid Composition, Dl Wetzel, Ta Sherwood, Ca Miller, Rl Medvecky
Red Drum Cewaf Uptake/Depuration Study: Lipid Composition, Dl Wetzel, Ta Sherwood, Ca Miller, Rl Medvecky
C-IMAGE data
Lipid class composition of red drum exposed to south Louisiana crude oil CEWAF using Iatroscan.
Cewaf-Exposed Florida Pompano Reproduction: Spawn And Hatch & Reproductive Function Assays, Dl Wetzel, Ta Sherwood, Nr Rhody, Mj Resley, Rl Medvecky
Cewaf-Exposed Florida Pompano Reproduction: Spawn And Hatch & Reproductive Function Assays, Dl Wetzel, Ta Sherwood, Nr Rhody, Mj Resley, Rl Medvecky
C-IMAGE data
Evaluation of fecundity, fertility, hatch success and larval survival to determine reproductive success. Reproductive steroid and protein concentrations in adult pompano after reproduction and exposure to chemically enhanced water accommodated fraction (CEWAF) of south Louisiana crude oil.
Red Drum Diet Exposure To Deepwater Horizon Crude Oil Contaminated Feed: Fish Growth, Dl Wetzel, Ta Sherwood, Rl Medvecky
Red Drum Diet Exposure To Deepwater Horizon Crude Oil Contaminated Feed: Fish Growth, Dl Wetzel, Ta Sherwood, Rl Medvecky
C-IMAGE data
This dataset contains RNA/DNA ratios of subadult red drum (Sciaenops ocellatus) that were fed a diet of pellets saturated with south Louisiana crude oil.
Total Polycyclic Aromatic Hydrocarbon Results For Cewaf-Exposed Florida Pompano Reproduction, Dana Wetzel, Tracy Sherwood, Rebecca Medvecky, Christelle Miller
Total Polycyclic Aromatic Hydrocarbon Results For Cewaf-Exposed Florida Pompano Reproduction, Dana Wetzel, Tracy Sherwood, Rebecca Medvecky, Christelle Miller
C-IMAGE data
Controlled oil exposure study to evaluate the effects of south Louisiana crude oil CEWAF exposure on Florida pompano health and reproduction. Data include total polycyclic aromatic hydrocarbon (tPAH) results for exposure solutions, pompano livers, pompano whole bodies, and pompano eggs, and VOC results for exposure solutions of F0 generation adults after six-month recovery/maturation period and spawning.
Dispersant Enhances Hydrocarbon Degradation And Alters The Structure Of Metabolically Active Microbial Communities In Shallow Seawater From The Northeastern Gulf Of Mexico, Xiaoxu Sun, Lena Chu, Elisa Mercando, Isabel Romero, David Hollander, Joel E Kostka
Dispersant Enhances Hydrocarbon Degradation And Alters The Structure Of Metabolically Active Microbial Communities In Shallow Seawater From The Northeastern Gulf Of Mexico, Xiaoxu Sun, Lena Chu, Elisa Mercando, Isabel Romero, David Hollander, Joel E Kostka
C-IMAGE Publications
Dispersant application is a primary emergency oil spill response strategy and yet the efficacy and unintended consequences of this approach in marine ecosystems remain controversial. To address these uncertainties, ex situ incubations were conducted to quantify the impact of dispersant on petroleum hydrocarbon (PHC) biodegradation rates and microbial community structure at as close as realistically possible to approximated in situ conditions [2 ppm v/v oil with or without dispersant, at a dispersant to oil ratio (DOR) of 1:15] in surface seawater. Biodegradation rates were not substantially affected by dispersant application at low mixing conditions, while under completely dispersed conditions, biodegradation …