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Metabolic Toxicity Studies Using High Throughput Bioreactor Assay And Liquid Chromatography Coupled With Mass Spectrometry (Lc-Ms/ Ms), Dandan Li

Doctoral Dissertations

Metabolic toxicity is one of main concerns in drug discovery and development. High efficient and low cost metabolic toxicity assessment is still a major challenge for pharmaceutical industry. This thesis focuses on the development of high throughput in vitro metabolic toxicity assay coupling with liquid chromatography tandem mass spectrometry for metabolic adducts generation, characterization and quantitation. The key technology was based on electrostatic fabrication of polyelectrolytes and biomolecules on magnetic beads to form bioreactors in 96 well plate format and acquired metabolites induced toxicity molecular information by LC-MS experiments.

Chapter one will address the goals and significance of the metabolic …

Poly(N-Vinylcaprolactam) Based Thermoresponsive Nanostructured Materials, Xing Liang

All ETDs from UAB

Poly(N-vinylcaprolactam) (PVCL) is a temperature-responsive polymer displaying a low critical solution temperature (LCST) around 30 to 40℃ in aqueous solutions that is close to the human physiological temperature. In addition, compared to other ther-moresponsive polymers, PVCL is nontoxic and biocompatible. In this thesis, we will fo-cus on the synthesis, fabrication and characterization of PVCL-based thermoresponsive nanostructured materials, such as films, hydrogels and block copolymers and their poten-tial applications in biomedical and medical areas. In chapter 2, we report on nanothin multilayer hydrogels of cross-linked PVCL that exhibited distinctive and reversible thermoresponsive behavior. The single-component PVCL hydrogels were produced by selective …

Layer-By-Layer Assemblies For Membrane-Based Enzymatic Catalysis, Andrew R. Tomaino

Theses and Dissertations--Chemical and Materials Engineering

While considerable progress has been made towards understanding the effect that membrane-based layer-by-layer (LbL) immobilizations have on the activity and stability of enzymatic catalysis, detailed work is required in order to fundamentally quantify and optimize the functionalization and operating conditions that define these properties. This work aims to probe deeper into the nature of transport mechanisms by use of pressure-induced, flow-driven enzymatic catalysis of LbL-functionalized hydrophilized poly(vinyldiene) (PVDF)-poly(acrylic acid) (PAA)-poly(allylamine hydrochloride) (PAH)-glucose oxidase (GOx) membranes. These membranes were coupled in a sealed series following cellulose acetate (CA) membranes for the elimination of product accumulation within the feed-side solution during operation. …