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Reverse Transcription Of 16s Rrna To Monitor Ribosome-Synthesizing Bacterial Populations In The Environment, Ting Lu, Peter George Stroot, Daniel B. Oerther
Reverse Transcription Of 16s Rrna To Monitor Ribosome-Synthesizing Bacterial Populations In The Environment, Ting Lu, Peter George Stroot, Daniel B. Oerther
Civil, Architectural and Environmental Engineering Faculty Research & Creative Works
Identification and quantification of phylogenetically defined bacterial populations in the environment are often performed using molecular tools targeting 16S rRNA. Fluorescence in situ hybridization has been used to monitor the expression and processing of rRNA by targeting the 3' tail of precursor 16S rRNA. To expand this approach, we employed reverse transcription of total RNA using primer S-D-Bact-0338-a-A-18. Length heterogeneity detected by slab gel analysis, denaturing high-performance liquid chromatography (DHPLC) was used to differentiate the 5' tail of the precursor from mature 16S rRNA, and the relative abundance of the precursor compared to the abundance of mature 16S rRNA was …
Controls On Microbial Community Structure In Thermal Environments : Exploring Bacterial Diversity And The Relative Influence Of Geochemistry And Geography, Kendra Renee Mitchell
Controls On Microbial Community Structure In Thermal Environments : Exploring Bacterial Diversity And The Relative Influence Of Geochemistry And Geography, Kendra Renee Mitchell
Biology ETDs
Community wide molecular surveys have revealed incredible hidden phylogenetic and metabolic diversity in microbial habitats. We have conducted the first microbial survey of Yellowstone National Park thermal environments, sampling 103 communities from across the park and across the range of conditions found. Yellowstone is particularly suited for this type of research because of the large number and wide variety of thermal springs, which are naturally occurring chemostats enabling examination of the factors that control microbes and drive community structure. In addition to samples for molecular microbial analysis, we collected water for extensive geochemical analysis in order to begin to deduce …
Identity And Diversity Of Agaricomycetes (Fungi: Basidiomycota) In Temperate Agricultural Soils, Barbara D. Bahnmann
Identity And Diversity Of Agaricomycetes (Fungi: Basidiomycota) In Temperate Agricultural Soils, Barbara D. Bahnmann
Digitized Theses
The objectives of this study were to identify and to compare the species richness, community composition and genetic diversity of Agaricomycetes in Michigan agricultural soils. Ribosomal DNA sequences were obtained from soils in four treatments: conventional till monocrop, no-till monocrop, historically tilled grassland and never-tilled grassland. The Agaricales dominated the community, with 85 % of all detected taxa. The taxonomic composition of conventional till plots was different from other plots due to the high prevalence of Pneumatospora (Cantharellales) and Lachnella/Calathella (Agaricales) clades and the absence of Hygrocybe (Agaricales) and Polyporales clades. These latter clades dominated less disturbed treatments. Genetic diversity …