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Microvascular Endothelial Cells Of The Bovine Corpus Luteum: A Comparative Study Of The Estrous Cycle And Pregnancy, Jessica A. Cherry

Master's Theses and Capstones

Endothelial cells derived from the corpus luteum (CLENDOs) exhibit a diverse array of characteristics that seemingly complement their wide-ranging properties in luteal function and fate. Reproductive status of the animal (i.e., non-pregnant vs. pregnant) may contribute to the phenotypic/physiologic diversity of microvascular endothelial cells, but information in this regard is limited. Here, structural and functional attributes of CLENDOs derived from the estrous cycle (Days 9-12) were compared with those of pregnancy (Day 60). Initially, lectin binding properties were examined in the cells. Using fluorescent-tagged lectins for Bandeiraea simplicifolia I or concanavalin A, flow cytometric analysis indicated that lectin-binding on CLENDOs …

The Expression Of Heat Shock Protein 70, Heat Shock Protein 90 And Heat Shock Factor 1 Within The Bovine Midcycle Corpus Luteum After Stimulation With Temperature In Vitro And Pgf2alpha In Vitro And In Vivo, Melissa N. Kopka

Master's Theses and Capstones

Environmental and biological stressors affect cellular function. Heat shock proteins (HSPs) are molecular chaperones that safeguard cells against these stressors by protecting endogenous proteins from degradation. Heat shock factors (HSF) are their transcriptional regulators. In the present study, we evaluated whether heat (Experiment 1) or prostaglandin F2alpha (PGF2alpha; Experiments 2 and 3) induces the expression of HSP 70, HSP 90 and HSF1 in the bovine corpus luteum (CL). In summary, in vitro heat treatment elicited a later response in luteal cells by increasing HSP 70 expression at 24 hrs, while in vivo PGF2alpha treatment leads to an earlier response by …

Expression Profiling Of The Spermidine Synthase3 (Spds3) And Spermine Synthase ( Spms) Genes During The Life Of Arabidopsis, Charles F. Rice

Master's Theses and Capstones

Polyamines are low molecular weight nitrogenous compounds found in all living organisms. These omnipresent molecules have been extensively studied in plant systems and have been implicated in a number of physiological responses including growth, development, and stress response. The proposed role of polyamines in vital processes such as cell division and differentiation has sparked enthusiasm in further investigation of this relatively small biosynthetic pathway. Very little is known about the regulation of polyamine biosynthetic genes, an aspect that can elucidate further functions of these ubiquitous compounds. In the following study, the expression profiles of two of these genes, spermidine synthase3 …

The Effects Of Photoperiod And Water Temperature On The Reproductive Development Of Black Sea Bass, Centropristis Striata, Katie Strait

Master's Theses and Capstones

Black sea bass are a potential candidate for marine aquaculture due to their high market value, high demand, and limited seasonal supply. One of the most significant impediments to commercial production of black sea bass is the lack of a year-round supply of eggs and larvae. Further, control of reproductive development is poorly understood in this species. Black sea bass are protogynous hermaphrodites that change sex between 2-5 years of age. It is unclear why adult black sea bass undergo sex change in the wild. Therefore, the first part of study sought to determine if density is involved in sex …

Analysis Of Adc2 And Samdc1 Expression And The Effects Of Transgenic Manipulation Of Polyamine Biosynthesis On Flower Development In Arabidopsis, Vasavi Rani Challa

Master's Theses and Capstones

Polyamines are low molecular weight, aliphatic cationic molecules playing a vital role in all cellular and physiological processes such as cell growth, differentiation, abiotic stress, and somatic embryogenesis. The present study is aimed at unraveling their role during floral development in Arabidopsis thaliana by studying the temporal and spatial expression of ADC2 and SAMDC1 genes by promoter-reporter fusion analysis, and by the transgenic expression of mouse ODC and carrot SAMDC under tissue specific promoters AP1 and AP3, respectively. The expression of SAMDC1 was found to be prominent during different stages of development and in different whorls of flowers whereas ADC2 …

Mhc (B) Complex Recombinant Immune Response, Nicole Gay Wilkinson

Master's Theses and Capstones

Recombination between chicken major histocompatibility complex (MHC) class I B-F and class IV B-G genes has enabled more precise identification of genes controlling immune responses. Congenic lines, each containing a unique MHC recombinant, were developed with 99.9% genetic uniformity to inbred Line UCD 003 (B17B17). A new recombinant, R13 (BF17-BG23), originated in the tenth backcross generation for R1 (BF24-BG23). Two experiments tested the R13 immune response to bovine red blood .cells (BRBC) and Rous sarcoma virus (RSV).

A single R13B17 sire mated to five R131317 dams produced progeny segregating for R13R13, R13B17, and B17B17 genotypes. These progeny were injected with …

Temporal Expression Of Protein Mediators During Pgf2alpha-Induced Luteolysis In Sheep, Bryon F. Ricketts

Master's Theses and Capstones

To study dynamic in vivo biochemical changes within the corpus luteum (CL) during luteolysis, a physiological model mimicking the onset of natural luteolysis was established, in which sheep received sequential systemic pulses of PGF2alpha (20ug/min/lhr) at mid-luteal phase of the estrous cycle. We previously demonstrated that after one pulse of PGF2alpha, the protein levels of extracellular matrix (ECM) regulators, tissue inhibitors of metalloproteinases (TIMPs) -1 and -2 decreased dramatically within one hour of PGF2alpha infusion, while matrix metalloproteinase (MMP)-2 activity increased 4 hrs post infusion. In summary, the early and dramatic decrease in TIMP-1 and TIMP-2 proteins, accompanied by an …

Activation Of Toll-Like Receptor 2 Through Tlr2-Lrr Binding Synthetic Peptides, Christopher Benton

Master's Theses and Capstones

Bacterial sepsis and systemic inflammatory immune responses continue to be major causes of illness and death despite extensive research into the development of antimicrobial agents. There is a need for novel therapeutic reagents designed to modulate these responses. Toll-like receptor 2 plays a key role in the development of innate and adaptive immune responses to microbial products that interact with the receptor's extracellular leucine-rich repeat ligand-binding domain. In a preliminary study, five peptides were synthesized that bound to the leucine-rich repeat region of Toll-like receptor 2 (TLR2). We tested the hypothesis that the novel TLR 2 leucine rich repeat binding …

Patterns And Processes Of Soil Carbon Dynamics In A Northeastern United States Forest, Sarah K. Silverberg

Master's Theses and Capstones

Forest soils represent a substantial component of the terrestrial carbon cycle and are an important research area for a number of carbon cycle science initiatives. Whereas patterns of aboveground productivity have been relatively well measured and are increasingly included in regional-scale model analyses, belowground estimates are still highly uncertain and progress has been hampered by methodological difficulties. The lack of data poses a problem because belowground measurements are needed to create complete carbon budgets for terrestrial ecosystems at local, regional and global scales. Ecosystem carbon balances will help identify how and where carbon is being stored, as well as how …

A Test Of The Nutrient-Productivity Model In The Gulf Of Maine Using The Intertidal Mussel Mytilus Edulis, Meredith A. Bailey

Master's Theses and Capstones

Historical concepts of top-down control (predator-prey interactions) on rocky intertidal community structure have transitioned to studies on bottom-up effects (nutrient supply and larval transport) as significant factors affecting rocky intertidal community structure. Studies performed on rocky intertidal locations along the Gulf of Maine (GOM) at multiple sites and seasons in 2004 and 2006 examined the ecology of Mytilus edulis populations by measuring size frequency distributions, diet quality (stable isotope composition) and physiological performance of individuals using condition indices and RNA/DNA ratios. Data were correlated to satellite imagery for sea surface temperature and chlorophyll a concentration and individuals were genetically tested …

Correlating Sea Otter Density And Behavior To Habitat Attributes In Prince William Sound, Alaska: A Model For Prediction, Heather A. Coletti

Master's Theses and Capstones

As a benthic foraging marine mammal, sea otters ( Enhydra lutris) present a unique opportunity for conducting a quantitative assessment of behavior based on habitat use as well as developing a habitat based density model using GIS because of the sea otter's well defined habitat requirements. Several studies have documented sea otter behavior but none have calculated the probability of occurrence of a particular behavior based on habitat attributes. Previous predictive models of sea otter density have been constructed, however these models have excluded offshore habitat. Seven aerial surveys, that included offshore habitats, were conducted between 1995 and 2005 in …

Characteristics And Dynamics Of A Moose Population In Northern New Hampshire, Anthony Richard Musante

Master's Theses and Capstones

Abundance indices suggest that the moose (Alces alces) population in northern New Hampshire has stabilized despite favorable habitat and conservative harvest. Natural mortality of unknown cause is presumed a primary reason although little is known about moose reproduction and survival in New Hampshire. This study (2002-2005) was designed to investigate the dynamics that impact this population.

Analysis of harvest reproductive data (1988-2004) indicated that average field-dressed weight of adult cows increased although corpora lutea count declined from ∼1.4- 1.2/cow in the study area and statewide. Yearling ovulation rate (∼42%) and average weight (<211 kg) declined about 25% and 4%, respectively. Parturition of radio-marked cows ranged from 8 May-13 July (median=19 May) with 78% of births from 13-27 May. Calving rate of yearlings and adults (>2 yr) averaged 30 and 85%, respectively, …

Salmonella Enterica Serovar Typhimurium Promotes Apoptosis In Activated Neutrophils, Christopher C. Wrocklage

Master's Theses and Capstones

Apoptosis is an extremely important and highly regulated function in neutrophils. As the most abundant, but shortest-lived white blood cells, they must initiate this process in the absence of pathogens and selectively release their potent cytotoxic components when pathogens are present. Many pathogens are able to modulate this process in neutrophils, either for the host's benefit or for their own. Previous work has shown that transmigration across a T84 intestinal epithelial crypt cell monolayer increases the ability of neutrophils to bind and kill Salmonella enterica serovar Typhimurium (S. Typhimurium).

The goal of this work was to see how S. Typhimurium …

The Development And Utilization Of Est (Expressed Sequence Tag) Resources In The Diploid Strawberry Model System, Robin Leigh Brese

Master's Theses and Capstones

Enhancement of genomic resources is needed for the cultivated octoploid strawberry Fragaria x ananassa (2n=8x=56). A diploid (2n=2x=14) relative, Fragaria vesca, has been used as a model system for strawberry genomics because of its small (~200 Mb) basic genome size, ancestry to the octoploid strawberries and availability of inbred lines, among other favorable features.

In this study, a cDNA library was constructed from developing flower buds of Fragaria vesca ssp. vesca cv. 'Yellow Wonder'. A cDNA library of 7680 clones was created using the CLONTECH SMART cDNA Library Construction kit. Sequencing and bioinformatic analyses of 3298 clones resulted in 2717 …

Studies Of Flower Color In Anagallis Monelli L, Andrea Quintana Pinilla

Master's Theses and Capstones

One of the main goals for plant breeders in the floriculture industry is to develop plants with new attributes. Characteristics such as flower size, growth habit, resistance to pests and diseases, flower size, and particularly flower color are among the most important traits to breed and improve. One of the best ways to make a plant popular in the market is to offer a wide variety of flower colors. A. monelli is an ornamental plant used for hanging baskets and as a bedding plant, and until recently, cultivars available were only blue- or orange-flowered. Recent breeding research on this species …

Abiotic Retention Of Nitrogen And Dissolved Organic Matter By Forest Mineral Soils, Naoko Watanabe

Master's Theses and Capstones

The effect of NO3- deposition on NH4 +, DON, and DOC retention as well as abiotic NO3 - retention via the hypothesized "ferrous wheel" mechanism was examined in oxic and anoxic tropical and oxic temperate soils using sorption isotherm experiments. Adsorption of NH4+, DON, and DOC by mineral soils was examined in forest floor extracts with DOC levels of 0-50 mg/L. Experimental treatments included no added NO3-, 4mg/L of added NO3-, or 4 mg/L of NO3 - and 2 mg/L of Fe3+. Ferric iron was added since it would likely increase DOC retention in the context of podzolization, and it …

Toxin-Producing Microcystis Aeruginosa: A Trade-Off In The Vertical Distribution Of Three Daphnia Species As Predicted By The Ideal Free Distribution With Costs Model, Sonya Claudine Carlson

Master's Theses and Capstones

Daphnia hyalina, D. galeata, and D. ambigua were exposed to the cyanobacterium Microcystis aeruginosa in 30 cm temperature-stratified columns. Changes in egg ratio, lipid index, length, and growth were recorded over 3 day experiments. Spatial distributions were recorded during 6-hour constant light experiments and 3 day fluctuating light experiments. D. hyalina and D. galeata stayed in the upper half of the column in temperatures of 18--22°C when exposed to M. aeruginosa. D. ambigua remained between 10--19°C regardless of food type. For across all experiments, depth and temperature ranges were narrowest in treatments with M. aeruginosa. D. ambigua incurred costs to …

Calcium-Dependent Protein Kinases In Physcomitrella Patens, Kathryn Chervincky

Master's Theses and Capstones

Calcium-dependent protein kinases (CDPKs) are a group of plant serine-threonine kinases that are stimulated in response to increases in intracellular calcium concentrations. Physcomitrella patens is a moss and a member of the bryophyte family. Physcomitrella has gained popularity as a model research organism due to the simple morphology of the plant as well as many other desirable characteristics. My research utilized Physcomitrella to accomplish four goals. The first goal was to identify and obtain sequence for as many CDPKs as possible in Physcomitrella. The second goal was to confirm the calcium-activated protein kinase activity of the identified CDPKs. The third …

An Experimental Approach To Understanding The Impact Of Vernal Pool Buffer Size On Wood Frogs (Rana Sylvatica), Nicole Alexis Freidenfelds

Master's Theses and Capstones

Strategies to conserve vernal pool-dependent amphibians emphasize the maintenance of adequate areas (buffers) of terrestrial habitat surrounding the pool. I conducted a large-scale field study that specifically examined the effects of buffer width manipulation on adult and metamorph wood frogs at eleven vernal pools in a managed forest in central Maine. Buffer width treatments were 30 m or 100 m forest buffer surrounded by a 100 m clearcut, or no cut (reference). I encircled pools with drift fences and monitored wood frogs from April--November, 2004 and 2005 using pitfall traps and radio-telemetry. Buffer width had no effect on the number …

Assessing The Suitability Of The Eelgrass (Zostera Marina L) Deep Edge As An Indicator Of Water Clarity In Estuarine Systems, David Omer Rivers

Master's Theses and Capstones

The suitability of the seagrass deep edge as an indicator of water clarity was assessed by examining the sensitivity and specificity of this indicator to changes in light. Indicator specificity was assessed by examining the extent to which seasonal variation in environmental parameters affected the location of the deep edge at five eelgrass meadows in the Great Bay Estuary, New Hampshire. The mean location of the eelgrass deep edge was seasonally stable at three sites, confirming its suitability as an indicator. At two sites, the deep edge responded to long-term light reduction and changes in deep edge location of 1.3 …

Effects Of Stz Induced Hyperglycemia And Hyperlipemia On Ppargamma And Abca1 In A Hamster Model Of Atherogenesis, Craig Lamarca

Master's Theses and Capstones

Diabetes mellitus accelerates atherogenesis, but the exact mechanisms involved are unknown. This study examined the effects of hyperglycemia on the inflammatory transcription factor peroxisome proliferator activated receptor gamma (PPARgamma), known to be involved in atherogenesis, and the ATP binding cassette A1 (ABCA1) protein that is a downstream product of PPARgamma. ABCA1 is a membrane bound transporter that is involved in reverse cholesterol transport via high density lipoprotein cholesterol (HDL-C). Hamsters were divided into two groups; hyperlipemic animals fed a high fat/high cholesterol control diet (C) and hyperlipemic/hyperglycemic animals that received the high fat diet and were administered streptozotocin. Half of …

Effects Of Forest Clear Cutting On Spotted Salamander ( Ambystoma Maculatum) Migration, Jessica Susannah Veysey

Master's Theses and Capstones

Upland buffer zones are a proposed management tool for vernal-pool-breeding amphibians. Substantial validation of buffers, via experimental upland habitat disturbance, is lacking. Specifically, no studies have examined immediate effects of clear cutting on spotted salamander (Ambystoma maculatum ) migration. I used clear cutting to experimentally manipulate upland buffer widths at 11 vernal pools. I then radiotracked 40 adult spotted salamanders at these pools, and modeled their migration with mixed-effects regression. Mean maximum distance from the pool was 106.0 +/- 15.4 m (range = 1.6 to 427.6 m). At clear cut-treatment pools, mean percent of time in the cut was 27.2 …

Native Gastropods And Introduced Crabs: Shell Morphology And Resistance To Predation In The New England Rocky Intertidal Zone, Sarah Joanne Teck

Master's Theses and Capstones

The impact of non-native species is one of the most critical issues facing management and conservation today. When these invaders are generalist predators, their impacts on native communities can be a major restructuring force for ecosystems. A new voracious predator the Asian crab, Hemigrapsus sanguineus , joins another non-native established species, Carcinus maenas, along the majority of the New England coastline. What remains poorly understood is how the two introduced predators may modify local communities, especially considering their impact on native prey, such as the rough periwinkle snail, Littorina saxatilis. The goal of this research is to investigate the vulnerability …

Environmental Effects On Early Life Stages Of American Shad (Alosa Sapidissima) And Rainbow Smelt (Osmerus Mordax ), Kristen Fuda

Master's Theses and Capstones

Recruitment of American shad (Alosa sapidissima) and rainbow smelt (Osmerus mordax) has steadily declined over the last few decades, possibly due to the construction of physical impediments to migration and increases in anthropogenic pollution. In order to elucidate environmental parameters influencing early life stages of anadromous fish, both laboratory and field studies were conducted. The effects of abiotic factors, including dissolved oxygen (DO), pH, salinity, nitrate, and phosphate, on hatch and survival of larval and juvenile American shad and rainbow smelt were examined in laboratory studies. Field studies on shad emigration were conducted in the Exeter River, and studies on …