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Open Access. Powered by Scholars. Published by Universities.®

2006

Iowa State University

Agricultural Science

3' untranslated region

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Trans Regulation Of Cap-Independent Translation By A Viral Subgenomic Rna, Ruizhong Shen, Aurélie Mamisoa Rakotondrafara, W. Allen Miller Oct 2006

Trans Regulation Of Cap-Independent Translation By A Viral Subgenomic Rna, Ruizhong Shen, Aurélie Mamisoa Rakotondrafara, W. Allen Miller

Plant Pathology and Microbiology Publications

Many positive-strand RNA viruses generate 3′-coterminal subgenomic mRNAs to allow translation of 5′-distal open reading frames. It is unclear how viral genomic and subgenomic mRNAs compete with each other for the cellular translation machinery. Translation of the uncapped Barley yellow dwarf virus genomic RNA (gRNA) and subgenomic RNAI (sgRNAI) is driven by the powerful cap-independent translation element (BTE) in their 3′ untranslated regions (UTRs). The BTE forms a kissing stem-loop interaction with the 5′ UTR to mediate translation initiation at the 5′ end. Here, using reporter mRNAs that mimic gRNA and SgRNA1, we show that the abundant sgRNA2 ...