Digital Commons Network^™

Switchgrass For Biomass Feedstock In The Usa, Matt Sanderson, Paul Adler, Akwasi Boateng, Michael Casler, Gautam Sarath

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Switchgrass has received much study for biomass feedstock production and conversion through research funded by the US-DOE and USDA during the past two decades. We have an improved understanding of the adaptation of existing cultivars and new cultivars with yield and adaptation improvements are now appearing. We also have a good understanding of the agronomics of switchgrass production, mainly from its use as a forage crop. There remain several constraints to switchgrass use in bioenergy cropping systems, including reliable establishment methods to obtain productive stands in the first year, targeted fertilization and nutrient management techniques to efficiently use nitrogen fertilizer, …

Untemplated Oligoadenylation Promotes Degradation Of Risc-Cleaved Transcripts, Fadia Ibrahim, Jennifer Rohr, Won-Joong Jeong, Jennifer Hesson, Heriberto D. Cerutti

Center for Plant Science Innovation: Faculty and Staff Publications

In the best-characterized mechanism of RNAmediated silencing, small interfering RNAs (siRNAs), incorporated into the RNA-induced silencing complex (RISC), guide the endonucleolytic cleavage of complementary RNAs (1). In Drosophila melanogaster, these RISC-generated products are eventually degraded by exoribonucleases: Xrn1, a 5′-to-3′ exonuclease, and exosome, a 3′-to-5′ multisubunit exonuclease (2). Interestingly, in Arabidopsis thaliana and in mammals, an oligouridine or oligoadenine [oligo(U/A)] tail is added to the 5′ RNA fragments resulting from microRNA-directed cleavage (3). However, the biological role of this tail remains unclear.

Intsormil’S Global Impact: A Revolution In West African Sorghum Production, Intsormil

INTSORMIL Impacts and Bulletins

Sorghum and pearl millet are the staple grains for millions of people around the world, especially those residing in the semiarid margins of agricultural sustainability. For this reason, INTSORMIL has been working in the semiarid regions of Africa and Central America where it has been helping sorghum and millet farmers achieve the status of surplus grain production and the economic, social and health benefits thus provided. What impact has INTSORMIL had on the lives of these sorghum and millet farmers?

To determine the global impact of the USAID funded INTSORMIL program, Battelle, an independent agency, was chosen to conduct an …

Bovine Immune Response To Shiga-Toxigenic Escherichia Coli O157:H7, Mark A. Hoffman, Christian Menge, Thomas A. Casey, William Laegreid, Brad T. Bosworth, Evelyn A. Dean-Nystrom

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Although cattle develop humoral immune responses to Shiga-toxigenic (Stx⁺) Escherichia coli O157:H7, infections often result in long-term shedding of these human pathogenic bacteria. The objective of this study was to compare humoral and cellular immune responses to Stx⁺ and Stx^- E. coli O157:H7. Three groups of calves were inoculated intrarumenally, twice in a 3-week interval, with different strains of E. coli: a Stx2- producing E. coli O157:H7 strain (Stx2⁺O157), a Shiga toxin-negative E. coli O157:H7 strain (Stx^-O157), or a nonpathogenic E. coli strain (control). Fecal shedding of Stx2⁺O157 was …

Agronomic And Quality Effects In Winter Wheat Of A Gene Conditioning Resistance To Wheat Streak Mosaic Virus, L. A. Divis, R. A. Graybosch, C. J. Peterson, P. Stephen Baenziger, G. L. Hein, B. B. Beecher, T. J. Martin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Wheat streak mosaic virus (WSMV) is one of the most important diseases limiting winter wheat (Triticum aestivum L.) production in the western Great Plains of North America. There is no known effective WSMV resistance within the primary gene pool of wheat. However, a resistance gene (Wsm1) has been transferred to wheat from a perennial relative, intermediate wheat-grass [Thinopyrum intermedium (Host) Barkworth & DR Dewey]. Nebraska-adapted winter wheat lines carrying Wsm1 were used to characterize the effects of this alien introgression on agronomic and quality traits. Sister-lines from six breeding populations were evaluated under virus-free conditions, …

United States Patent Application Publication: Counter Selection Strategy For Gram-Negative Bacteria, Stephen K. Farrand, Paul E. Staswick, Thomas E. Clemente

Center for Plant Science Innovation: Faculty and Staff Publications

A Gram-negative bacterium useful for genetically engineering plants is provided. The Gram-negative bacterium contains, as part of genome, an inducible regulatory sequence operatively linked to a nucleotide sequence encoding a levansucrase. Alternatively, the Gram-negative bacterium comprises a recombinant nucleic acid construct containing an inducible regulatory sequence operatively linked to a nucleotide sequence encoding a levansucrase. Also provided are recombinant nucleic acid constructs comprising an inducible regulatory sequence operatively coupled to a nucleotide sequence encoding a levansucrase and a method for transforming plants using the Gram-negative bacterium of the present invention.

Nuclec Acds Encoding Pseudomonas Hop Proteins And Use Thereof, Alan Collmer, James R. Alfano, Xiaoyan Tang, C. Robin Buell, Gregory B. Martin

Department of Plant Pathology: Faculty Publications

The present invention relates to isolated nucleic acid mol ecules encoding a type III—secreted bacterial protein capable of modifying a cell death pathway in a plant cell. One aspect of the present invention involves an isolated nucleic acid molecule having a nucleotide sequence that encodes the HopPtol)2 protein of Pseudomonas syringae pv. syringae DC 3000. Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. The nucleic acid mol ecules of the present invention can be used to impart disease resistance to a plant and to make a plant hypersusceptible to …

The Circuitous Path To The Comparison Of Simulated Values From Crop Models With Field Observations, Albert Weiss, Wally Wilhelm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Journal of Agricultural Science, Cambridge has been a fixture in dissemination of crop simulation models and the concepts and data upon which they are built since the inception of computers and computer modeling in the mid-20th century. To quantify the performance of a crop simulation model, model outputs are compared with observed values using statistical measures of bias, i.e. the difference between simulated and observed values. While applying these statistical measures is unambiguous for the experienced user, the same cannot always be said of determining the observed or simulated values. For example, differences in accessing crop development can be …

Whole-Genome Expression Profiling Defines The Hrpl Regulon Of Pseudomonas Syringae Pv. Tomato Dc3000, Allows De Novo Reconstruction Of The Hrp Cis Element, And Identifies Novel Coregulated Genes, Adriana O. Ferreira, Christopher R. Myers, Jeffrey S. Gordon, Gregory B. Martin, Monica Vencato, Alan Collmer, Misty D. Wehling, James R. Alfano, Gabriel Moreno-Hagelsieb, Warren F. Lamboy, Geneviene Declerck, David J. Schneider, Samuel W. Cartinhour

Department of Plant Pathology: Faculty Publications

Pseudomonas syringae pv. tomato DC3000 is a model pathogen of tomato and Arabidopsis that uses a hypersensitive response and pathogenicity (Hrp) type III secretion system (T3SS) to deliver virulence effector proteins into host cells. Expression of the Hrp system and many effector genes is activated by the HrpL alternative sigma factor. Here, an open reading frame-specific whole-genome microarray was constructed for DC3000 and used to comprehensively identify genes that are differentially expressed in wild-type and ΔhrpL strains. Among the genes whose differential regulation was statistically significant, 119 were upregulated and 76 were downregulated in the wild-type compared with the ΔhrpL …

University Research Program Sees Drop In Funding, Zach Pluhacek

INTSORMIL Impacts and Bulletins

A program headquartered at the University of Nebraska-Lincoln that uses science to assist economically stressed nations will receive $9 million throughout five years through a cooperative agreement with the U.S. Agency for International Development.

The agreement, announced Friday morning by the UNL Office of Research and Graduate Studies, will significantly decrease the annual funding the International Sorghum and Millet Collaborative Research Support Program, or INTSORMIL, has received in recent years.

Sorghum Flour In The El Salvador Baking Industry, Intsormil

INTSORMIL Impacts and Bulletins

The El Salvador baking industry is dependent on imported wheat which results in a loss of valuable foreign exchange. In response, CENTA (Centro Nacional de Tecnologia Agropecuaria y Forestal) scientists with support from INTSORMIL have produced sorghum varieties which have the physical and chemical qualities making them suitable for flour which can be used as a partial substitute for wheat flour in the baking industry, thus decreasing the cost of baked goods. CENTA food technician, Fidelia Herrera pioneered the use of sorghum flour in El Salvador and began helping village bakers utilize sorghum flour back in the 70’s.

To test …

Harina De Sorgo En La Industria Panificadora De El Salvador, Intsormil

INTSORMIL Impacts and Bulletins

La industria panificadora de El Salvador depende de las importaciones de trigo, lo cual ocasiona una pérdida considerable de divisas. En respuesta a esto, los investigadores del Centro Nacional de Tecnología Agropecuaria y Forestal (CENTA) con ayuda del INTSORMIL han venido produciendo variedades de sorgo con cualidades físicas y químicas adecuadas para sustituir parcialmente la harina de trigo, reduciendo de esta manera los costos de producción de las panaderías. La Señora Fidelia Herrera, técnologa del CENTA fue la primera en tomar el liderazgo sobre la necesidad del uso de la harina de sorgo en El Salvador, al ayudar durante los …

Grazing Management Effects On Sediment And Phosphorus In Surface Runoff, Matthew M. Haan, James R. Russell, Wendy J. Powers, John L. Kovar, Jamie L. Benning

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Sediment and phosphorus (P) in runoff from pastures are potential non–point-source pollutants in surface waters that may be influenced by surface cover, sward height, treading damage, surface slope, soil moisture, and soil P. The objectives of the current study were to quantify sediment and total P loads in runoff produced during simulated rainfall from pastures and to evaluate their relationships with the physical and chemical characteristics of the soil and sward. Five forage management treatments— ungrazed (U), hay harvest/fall stockpile grazing (HS), continuous stocking to a sward height of 5 cm (5C), and rotational stocking to sward heights of 5 …

Convergence Of Agriculture And Energy: Implications For Research And Policy, Kenneth Cassman, Vernon Eidman, Eugene Simpson, Larry Berger, Robert Loomis, Marie Walsh, Todd A. Peterson, Edward Runge

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Access to an adequate energy supply at reasonable cost is crucial for sustained economic growth. Unfortunately, oil prices and the need to import from politically unstable countries lowers the reliability of the U.S. energy supply and hinders economic development. Although biofuels have been identified as an important component of the national strategy to decrease U.S. dependence on imported oil, the ability to sustain a rapid expansion of biofuel production capacity raises new research and policy issues. This document seeks to identify the most critical of these issues to help inform the policy development process. The goal is to enhance the …

Comparison Of Heat Tolerance Of Feedlot Heifers Of Different Breeds, T. M. Brown-Brandl, J. A. Nienaber, Roger A. Eigenberg, Terry L. Mader, J. L. Morrow, J. W. Dailey

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Heat stress in cattle causes decreases in feed intake and feed efficiency; in extreme cases, it can cause death. These losses amount to millions of dollars each year. A study was designed to determine severity of heat stress among four breeds of cattle. Throughout two summers, 256 feedlot heifers of four different breeds were observed. Respiration rates, panting scores, and surface temperatures were taken twice each day on 10 animals/breed for several weeks during the summers of 2002 and 2003. Twenty-four-hour behavior measurements were recorded for four heat-stress and four thermoneutral days. Results showed during the afternoon, Angus cattle (black) …

Heat Stress Risk Factors Of Feedlot Heifers, T. M. Brown-Brandl, Roger A. Eigenberg, J. A. Nienaber

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Heat stress in cattle results in millions of dollars in lost revenue each year due to production losses, and in extreme cases, death. Death losses are more likely to result from animals vulnerable to heat stress. A study was conducted to determine risk factors for heat stress in feedlot heifers. Over two consecutive summers, a total of 256 feedlot heifers (32/ breed/ year) of four breeds were observed. As a measure of stress, respiration rates and panting scores were taken twice daily (morning and afternoon) on a random sample of 10 heifers/ breed. Weights, condition scores, and temperament scores were …

Heat Stress Risk Factors Of Feedlot Heifers, Tami M. Brown-Brandl, Roger A. Eigenberg, John A. Nienaber

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Heat stress in cattle results in millions of dollars in lost revenue each year due to production losses, and in extreme cases, death. Death losses are more likely to result from animals vulnerable to heat stress. A study was conducted to determine risk factors for heat stress in feedlot heifers. Over two consecutive summers, a total of 256 feedlot heifers (32/ breed/ year) of four breeds were observed. As a measure of stress, respiration rates and panting scores were taken twice daily (morning and afternoon) on a random sample of 10 heifers/ breed. Weights, condition scores, and temperament scores were …

Microbial Content Of Abattoir Wastewater And Its Contaminated Soil In Lagos, Nigeria, A. O. Adesemoye, B. O. Opere, S. C. O. Makinde

Department of Plant Pathology: Faculty Publications

Microbial content of wastewater in two abattoirs and the impact on microbial population of receiving soil was studied in Agege and Ojo Local Government Areas in Lagos State, Nigeria. Wastewater samples were collected from each of the abattoirs over three months period and examined for microbial content. Soil samples contaminated with the wastewaters were also collected and analyzed for microbial content as compared to soil without wastewater contamination in the neighborhood (control). Some physico-chemical parameters of the samples such as total dissolved solid, chemical oxygen demand, etc., were examined. The wastewater samples from both abattoirs were highly contaminated; Agege abattoir …

Genetic Characterization And Diversity Of Rathayibacter Toxicus, Irina V. Agarkova, A. K. Vidaver, E. N. Postnikova, I. T. Riley, N. W. Schaad

Department of Plant Pathology: Faculty Publications

Rathayibacter toxicus is a nematode-vectored gram-positive bacterium responsible for a gumming disease of grasses and production of a highly potent animal and human toxin that is often fatal to livestock and has a history of occurring in unexpected circumstances. DNA of 22 strains of R. toxicus from Australia were characterized using amplified fragment length polymorphism (AFLP) and pulsed-field gel electrophoresis (PFGE). AFLP analysis grouped the 22 strains into three genetic clusters that correspond to their geographic origin. The mean similarity between the three clusters was 85 to 86%. PFGE analysis generated three different banding patterns that enabled typing the strains …

Emended Classification Of Xanthomonad Pathogens On Citrus, Norman W. Schaad, Elena Postnikova, George Lacy, Aaron Sechler, Irina V. Agarkova, Paul E. Stromberg, Verlyn K. Stromberg, Anne K. Vidaver

Department of Plant Pathology: Faculty Publications

In the paper by Schaad et al. [24] on reclassification of several xanthomonads, nomenclatural errors were made. The name Xanthomonas smithii subsp. citri proposed for the former taxon X. campestris pv. citri ( = X. axonopodis pv. citri) is illegitimate. Following the reinstatement of X. citri (ex Hasse 1915) Gabriel et al. [9] as a validly published name, Young et al. [34] wrote that the reinstatement of this epithet was based on a description that was inadequate in terms of modern practice for the purpose of formal classification. This report was subsequently summarized by the International Committee on …

Panadería “Pan Rey,” El Salvador Prueba Harina De Sorgo, Intsormil

INTSORMIL Impacts and Bulletins

Pan Rey, una panadería grande y comercializada en Quezaltepeque, El Salvador C. A. viene experimentando con la harina de sorgo como substituto de la harina de trigo, y en colaboración con el Laboratorio de Tecnología de Alimentos del CENTA (Centro Nacional de Tecnología agropecuaria) con el fín de promover el uso de la harina de sorgo en las panaderías grandes. Muchas de las panaderías pequeñas del sector rural, como la panadería de Clemencia Barrera en San Rafael Cedros, El Salvador (ver Reporte No.5 de INTSORMIL), han substituido la harina de trigo por la de sorgo durante muchos años. Sin embargo, …

Pan Rey Bakery, El Salvador Tests Sorghum Flour, Intsormil

INTSORMIL Impacts and Bulletins

Pan Rey, the “Bread King,” a large commercial bakery located at Quezaltepeque, El Salvador is testing sorghum flour as a substitute for wheat. The Pan Rey Bakery collaborates with the CENTA Food Technology Laboratory in a project to promote the use of sorghum flour in large bakeries. Many small, village level bakeries, such as the bakery of Clemencia Barrera in San Rafael Cedro, El Salvador (see INTSORMIL Report No. 5), have been using sorghum flour as a substitute for wheat for many years. However, use of sorghum flour on a large scale in the big commercial bakeries, such as Pan …

Center For Grassland Studies Newsletter, Fall 2006, Volume 12, No. 4

Center for Grassland Studies: Newsletters

Chemical Characteristics of Aging Golf Greens by Roch Gaussoin, Department of Agronomy and Horticulture, UNL

Can Ecophysiological Characteristics Explain the Success of Woody Species in the Sandhills of Nebraska? by Kathleen Eggemeyer, Tala Awada, F. Edwin Harvey, David Wedin, Xinhua Zhou and Sue Ellen Pegg, School of Natural Resources, UNL

Comparison of a Long Yearling System to Calf-fed Performance and Economics by Will Griffin and Terry Klopfenstein, Department of Animal Science, UNL

A Tale of Two States by Jana Beckman, Kansas Center for Sustainable Agriculture and Alternative Crops, K-State Research and Extension

2006 Nebraska Grazing Conference Best Yet!

Transcriptome Analysis Of Aspergillus Nidulans Exposed To Camptothecin-Induced Dna Damage, Iran Malazavi, Marcela Savoldi, Sonia Marla Zingaretti Di Mauro, Carlos Frederico Martins Menck, Steven D. Harris, Maria Helena De Souza Goldman, Gustavo Henrique Goldman

Department of Plant Pathology: Faculty Publications

We have used an Aspergillus nidulans macroarray carrying sequences of 2,787 genes from this fungus to monitor gene expression of both wild-type and uvsB^ATR (the homologue of the ATR gene) deletion mutant strains in a time course exposure to camptothecin (CPT). The results revealed a total of 1,512 and 1,700 genes in the wild-type and uvsB^ATR deletion mutant strains that displayed a statistically significant difference at at least one experimental time point. We characterized six genes that have increased mRNA expression in the presence of CPT in the wild-type strain relative to the uvsB^ATR mutant strain: fhdA …

Intsormil Crsp: Sorghum, Millet And Other Grains Collaborative Research Support Program: Leader With Associates Cooperative Agreement No. Eep-A-00-06-0016-00; Award From The U.S. Agency For International Development To The University Of Nebraska-Lincoln, Joseph Schmidt

INTSORMIL Scientific Publications

Pursuant to the authority contained in the Foreign Assistance Act of 1961, as amended, the U.S. Agency for International Development (USAID) hereby awards to University of Nebraska - Lincoln (hereinafter referred to as the "Recipient"), the sum of $9,000,000.00 to provide support for a program in Sorghum, Millet, and Other Grains Collaborative Research Support Program as described in the Schedule of this award and in Attachment B, entitled "Program Description."

This Leader with Associates Cooperative Agreement is effective and obligation is made as of 09-30-2006 and shall apply to expenditures made by the Recipient in furtherance of program objectives during …

Ecophysiology Of Two Native Invasive Woody Species And Two Dominant Warm-Season Grasses In The Semiarid Grasslands Of The Nebraska Sandhills, Kathleen D. Eggemeyer, Tala Awada, David A. Wedin, F. Edwin Harvey, Xinhua Zhou

School of Natural Resources: Faculty Publications

Populations of Pinus ponderosa and Juniperus virginiana are expanding into semiarid Sandhills grasslands in Nebraska. To evaluate the physiological basis of their success, we measured the seasonal course of leaf gas exchange, plant water status, and carbon isotope discrimination in these two native trees and two native C₄ grasses (Schizachyrium scoparium and Panicum virgatum). Compared to the trees, grasses had higher net photosynthetic rates (A_net) and water use efficiency (WUE) and more negative predawn and midday water potentials (Ψ) in June and July. While leaf Ψ and rates of leaf gas exchange declined for all …

Nitrate Accumulation And Movement Under Deficit Irrigation In Soil Receiving Cattle Manure And Commercial Fertilizer, D. D. Tarkalson, J. O. Payero, S. M. Ensley, Charles A. Shapiro

Department of Agronomy and Horticulture: Faculty Publications

Nitrate leaching from agricultural soils can increase groundwater nitrate concentrations. The objectives of the study were to assess the accumulation and movement of nitrate in the soil profile over a 2-year period under def¬icit irrigation conditions following a one time application of N in cattle feedlot manure and commercial fertilizer to corn at rates to achieve yield goals expected under conditions of full irrigation. Cattle manure and ammonium nitrate were applied in 2002 at the University of Nebraska recommended rate (1M and 1F, respectively) and cat¬tle manure was applied at twice the recommended rate (2M) for N for the 2002 …

Dna Molecules And Polypeptides Of Pseudomonas Syringae Hrp Pathogenicity Island And Their Uses: U.S. Patent No. Us 7,102,059 B2, Alan Collmer, James R. Alfano, Amy O. Charkowski

Department of Plant Pathology: Faculty Publications

One aspect of the present invention relates to isolated nucleic acid molecules (i) encoding proteins or polypeptides of Pseudomonas CEL and EEL genomic regions, (ii) nucleic acid molecules which hybridize thereto under stringent conditions, or (iii) nucleic acid molecules that include a nucleotide sequence which is complementary to the nucleic acid molecules of (i) and (ii). Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. Another aspect relates to the isolated proteins or polypeptides and compositions containing the same. The nucleic acid molecules and proteins of the present invention can …

Cloning Of Nt.Cviqii Nicking Endonuclease And Its Cognate Methyltransferase: M.Cviqii Methylates Ag Sequence, Siu-Hong Chan, Zhenyu Zhu, David Dunigan, James L. Van Etten, Shuang-Yong Xu

Department of Plant Pathology: Faculty Publications

Chlorella virus NY-2A has a large, highly methylated dsDNA genome (45% of the cytosines are 5-methylcytosine and 37% of the adenines are N⁶-methyladenine). Here, we report the cloning, expression, and characterization of the NY-2A-encoded CviQII nicking-modification (N-M) system. The nicking endonuclease, Nt.CviQII, recognizes R ↓ AG (R = A or G, ↓ indicating cleavage site) sequences and cleaves the phosphodiester bond 5′ to the adenosine. Because of the difficulty in cloning and expressing the wild-type Nt.CviQII, C-terminal truncation mutants were generated and full-length Nt.CviQII was reconstructed by intein-mediated peptide ligation. The truncation mutants and the reconstructed full-length Nt.CviQII …

Inducible Yeast System For Viral Rna Recombination Reveals Requirement For An Rna Replication Signal On Both Parental Rnas, Hernan Garcia-Ruiz, Paul Ahlquist

Department of Plant Pathology: Faculty Publications

To facilitate RNA recombination studies, we tested whether Saccharomyces cerevisiae, which supports brome mosaic virus (BMV) replication, also supports BMV RNA recombination. Yeast strains expressing BMV RNA replication proteins 1a and 2a^pol were engineered to transiently coexpress two independently inducible, overlapping, nonreplicating derivatives of BMV genomic RNA3. B33' lacked the coat protein gene and negative-strand RNA promoter. B35' lacked the positive-strand RNA promoter and had the coat gene replaced by the selectable URA3 gene. After 12 to 72 h of induction, B33' and B35' transcription was repressed and Ura⁺ yeast cells were selected. All Ura⁺ cells …