Digital Commons Network^™

Unlocking The Mysteries Of Weed And Mexico, Eddy Martinez

Capstones

An American biotech firm named Phylos Bioscience is interested in entering the Mexican marijuana market once it legalizes. Before it can do that, it must deal with the Mexican government and a conservative society.

https://eddymartinezcunyedu.atavist.com/figuring-out-the-mysteries-of-weed-and-mexico

Linkage Mapping For Soybean (Glycine Max) Flood Tolerance, Wade Stiles Hummer

Graduate Theses and Dissertations

Flood tolerance in soybean (Glycine max) is not a well-characterized trait, yet flooding damage is second only to drought stress in terms of yield reduction. The objectives of this study were to determine genetic variation for flooding tolerance in two populations of soybean recombinant inbred lines (RILs) and to identify and confirm flood tolerant QTL. Population A (WHA) consisted of 111 RILs derived from the cross 5002T by 91210-350 and Population B (WHB) consisted of 79 RILs from the cross RA-452 by Osage. Experiments were conducted at the Rice Research and Extension Center (RREC) near Stuttgart, AR in 2015 and …

Evaluation Of Genetic And Environmental Influences On Broiler Meat Quality, Sara Katherine Orlowski

Graduate Theses and Dissertations

The effect of both genetic and environmental influences on broiler meat quality were evaluated over three experiments. Selection response was assessed for broiler lines selected for high (HBY4) and low (LBY4) four day percentage breast yield which were formed from a random bred control (RAN). A modern random bred population (MRB) created in 2015 was evaluated for differences in 4 day percentage breast yield and the resulting differences in the incidence and severity of the woody breast and white striping myopathies. Additionally, the use of embryonic thermal manipulation to impact cell division and early caloric feed restriction to slow growth …

The Role Of Tumor Suppressor Dear1 In The Acquisition Of Mammary Stem/Progenitor Cell Properties, Uyen Le

Dissertations & Theses (Open Access)

Breast cancer is the most commonly diagnosed cancer in women in America. Ductal carcinoma in situ (DCIS), one of the earliest pre-invasive forms of invasive ductal carcinoma (IDC), has a 30-50% risk of progressing to IDC. Understanding the mechanisms regulating progression from DCIS to IDC would help identify biomarkers to stratify patients at higher risk of progression or metastasis. Cumulative literature suggests the earliest phase of dissemination from the primary tumor is driven by the epithelial-mesenchymal transition (EMT) program. DEAR1 is a tumor suppressor gene which is mutated, undergoes loss of heterozygosity in breast cancer, and is downregulated in DCIS …

Generation Of Isogenic Human Pluripotent Stem Cell-Derived Neurons To Establish A Molecular Angelman Syndrome Phenotype And To Study The Ube3a Protein Isoforms, Carissa Sirois

Doctoral Dissertations

Angelman Syndrome (AS) is a neurodevelopment disorder for which there is currently no cure that is characterized by severe seizures, intellectual disability, absent speech, ataxia, and happy affect. Loss of expression from the maternally inherited copy of UBE3A, a gene regulated by genomic imprinting, causes AS. Currently there are multiple promising therapeutic approaches being explored and developed for AS, some of which involve targeting or expression of the human genetic sequence. Subsequently, it is necessary to establish robust cellular models for AS that can be used to test these, as well as future, potential AS therapies. Toward this aim, …

Evaluation Of The Epigenetic Regulation Of Two X-Linked, Autism Candidate Genes: X-Linked Lymphocyte Regulated 3b And Transketolase-Like 1, Glenn W. Milton

Doctoral Dissertations

According to the CDC the prevalence of Autism Spectrum Disorder (ASD) in males compared to females is approximately 5:1. Skuse et al. hypothesized that imprinted genes on the X chromosome could account for this sex bias. Genomic imprinting is defined as differential gene expression dependent upon the parental origin of the allele. While genomic imprinting on autosomes has been well classified, no imprinting mechanism on the sex chromosomes has been discovered. This work presents an investigation into the regulation of expression governing the imprinted locus of X-Linked Lymphocyte Regulated 3b/4b/4c (Xlr3/4), and closely linked ASD candidate gene, Transketolase-Like …

Gene Expression Changes By Neuroprotectant Novel Antidotes To Organophosphates, Meghan L. Brino

Honors Theses

Recent assassinations and terrorist attacks demonstrate the need for a more effective antidote against nerve agents and other organophosphate (OP) compounds which inhibit the nervous system enzyme acetylcholinesterase (AChE). This study explored the changes in gene expression induced by novel phenoxyalkyl pyridinium oximes (U.S. patent 9,277,937) that have demonstrated the ability to cross the blood-brain barrier and attenuate brain damage in a rat model. More specifically, this study determined whether rats treated with high levels of a surrogate for the nerve agent sarin (nitrophenyl isopropyl methylphosphonate; NIMP) and rats treated with NIMP followed by a novel oxime displayed significant differences …

Genome Wide Association Studies For Identification Of Markers Linked To Sucrose Traits In Sugarcane, Nathanael Darrell Fickett

LSU Doctoral Dissertations

Sugarcane, being a complex aneu-polyploid, poses unique challenges to fine mapping of quantitative trait loci (QTL) controlling agronomic traits of interest. Genome-wide association studies (GWAS) could be a better alternative to identify molecular markers associated with specific traits due to high linkage disequilibrium in sugarcane. In the first study, marker-trait associations (MTAs) were evaluated for three sucrose traits, Brix, total recoverable sugar (TRS), and percent sucrose on 48 elite and historic Louisiana breeding clones using 1,062 alleles. These sucrose traits were highly correlated (P-value < 0.0001) at >0.96. TASSEL 5.1 and JMP Genomics 8.0 were compared with eight models run in each program. …

Genetic Dissection Of Non-Host Resistance To The Wheat Stem Rust Pathogen, Using An Interspecific Barberry Hybrid, Radhika Bartaula

Doctoral Dissertations

Stem rust, caused by the macrocyclic fungal pathogen P. graminis (Pg), is one of the most devastating diseases of wheat and other small grains globally; and the emergence of new stem rust races virulent on deployed resistance genes brings urgency to the discovery of more durable sources of genetic resistance. Given its intrinsic durability and effectiveness across a broad range of pathogens, non-host resistance (NHR) presents a compelling strategy for achieving long-term rust control in wheat. However, NHR to Pg (Pg-NHR) remains largely unexplored as a protection strategy in wheat, in part due to the challenge of developing a genetically …

Eco-Evolutionary Implications Of Environmental Change Across Heterogeneous Landscapes, Jared J. Homola

Electronic Theses and Dissertations

Species use a variety of mechanisms to adapt to environmental change. These range from spatially tracking optimal environments, to phenotypically plastic responses and evolutionary adaptation. Due to increases in anthropogenic influence on environments, characteristics of change such as their duration and magnitude are undergoing fundamental shifts away from the natural disturbance regimes that shaped species’ evolution. This dissertation uses empirical data and simulation models to examine the ecological and evolutionary consequences of environmental change across real, heterogeneous landscapes for multiple species, with an emphasis on anthropogenic changes. I used landscape genetics to evaluate the effects of urbanization on two native …

Hpa Axis Genetic Variation And Life Stress Influences On Functional Connectivity In Resting State Networks, Tara Ann Miskovich

Theses and Dissertations

Stressful or traumatic experiences are a key risk factor for developing psychopathology, primarily through the impact that chronic stress has on hypothalamic-pituitary-adrenal (HPA) axis functioning. The HPA axis regulates the stress response but can become dysregulated with chronic activation and impact brain functioning. In addition to environmental stressors, genetic variation in genes in the HPA axis appear to influence HPA axis functioning and is also related to disruption in brain functioning, particularly in the context of high life stress. The current study focused on examining potential mechanisms through which trauma and stress interacts with HPA axis genes to impact key …

Molecular Survey For The Honey Bee Trypanosome Parasites Crithidia Mellificae And Lotmaria Passim, Mary-Kate Williams

Graduate Theses and Dissertations

Honey bee populations have been fluctuating within recent years. No one cause has been attributed to colony fluctuations due to the theory that multiple stressors interact with one another to impact colony health. Consequently, microorganisms such as internal parasites of honey bees have been understudied as a contributor to colony health decline.

Molecular diagnostics were utilized to detect the presence of two honey bee trypanosome parasites, Crithidia mellificae and Lotmaria passim, in managed and feral honey bee populations from eight states in the United States (USA). Because studies on trypanosome infections are lacking in the USA, it is important to …

Investigation Of Bacterial Taxa As Biomarkers Of Fescue Toxicosis And Heat Stress In Grazing Beef Cows, Sarah Katherine Chewning

Graduate Theses and Dissertations

Fescue toxicosis in grazing beef cattle is caused by consumption of Ergot alkaloids associated with tall fescue (toxic fescue) and is responsible for substantial economic loss to the U.S. beef industry. Cattle consuming toxic fescue suffer adverse physiological responses, such as: hyperthermia, increased respiration rate (RR), poor reproduction and growth performance. Other adverse responses to fescue toxins include the retention of a winter hair coat and vasoconstriction in the extremities, which can lead to tissue necrosis. Identifying cattle with reduced susceptibility to fescue toxins would allow for efficient use of fescue pastures; thus, the objectives of this thesis was to: …

Hyper-Optimized Machine Learning And Deep Learning Methods For Geo-Spatial And Temporal Function Estimation, Neelabh Pant

Computer Science and Engineering Dissertations

Owing to a high degree of freedom in human mobility, accurate modelling/estimation of human mobility function remains a challenge. Numerous work in the literature have tried to address the challenge using various traditional machine learning methods on spatio-temporal attributes of data. We compare the use of Varied-K Means clustering, Hidden Markov Model techniques, feed forward neural networks, recurrent neural networks (RNN) and Long Short Term Recurrent Neural Networks (LSTM) to predict a user's future movement based on the user's past historical data. Although several techniques were proposed to predict a user's movement, not many have concentrated on a user's location …

Gene-Environment Interaction: Brain-Derived Neurotrophic Factor (Bdnf) As A Moderating Factor For The Effects Of Exercise And Diet On Cognitive And Mental Health: The Cache County Study On Memory In Aging, Chelsea L. Sanders

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Cache County Study on Memory in Aging, funded by the National Institute on Aging, studied longitudinal changes in memory and aging over 12 years’ follow-up in a population-based sample of 5,092 older adults in semirural Cache County, UT. Among the extensive interview procedures, researchers collected information regarding the participants’ demographics, health, genetic factors, diet, physical activity, and cognitive abilities. This study has allowed researchers to investigate how genetic and modifiable lifestyle factors interact to predict health, cognitive function, and psychological wellbeing in older adults.

Diet and exercise are important lifestyle factors in maintaining cognitive health and psychological wellbeing throughout …

Structural And Functional Brain Connectivity In Middle-Aged Carriers Of Risk Alleles For Alzheimer's Disease, Laura Korthauer

Theses and Dissertations

Single nucleotide polymorphisms (SNPs) in APOE, COMT, BDNF, and KIBRA have been associated with age-related memory performance and executive functioning as well as risk for Alzheimer’s disease (AD). The purpose of the present investigation was to characterize differences in brain functional and structural integrity associated with these SNPs as potential endophenotypes of age-related cognitive decline. I focused my investigation on healthy, cognitively normal middle-aged adults, as disentangling the early effects of healthy versus pathological aging in this group may aid early detection and prevention of AD. The aims of the study were 1) to characterize SNP-related differences in functional connectivity …

Lynch Syndrome, Raejeanne B. Nutter

Nursing Student Class Projects (Formerly MSN)

Lynch syndrome (LS) was previously known as Hereditary Non-polyposis colorectal cancer syndrome (HNPCC) (Shulman, 2015) is a hereditary cancer syndrome. The research explored Lynch Syndrome, the identification, and treatment. Lynch syndrome is associated with cancers of the ovary, stomach, urothelial tract, and small bowel, and less frequently, cancers of the brain, biliary tract, pancreas, and prostate. (Cox et al., 2018) “An individual’s lifetime risk for developing colorectal cancer (CRC) estimated at 5% to 6%; an individual with Lynch syndrome has an 80% lifetime risk. Lynch syndrome is an autosomal dominant disorder caused by germline mutations in 1 of 4 mismatch …

"What Is The M/F Problem?": Critical Discourse Analysis In Anne Fausto-Sterling's Sexing The Body: Gender Politics And The Construction Of Sexuality, Marysarah Grace Reid

Electronic Theses and Dissertations

Current dominant understandings of biological sex and gender that rely upon binary constructions are problematic on cultural, political, and individual levels. Cultural misunderstandings of the complexities of biological sex have harmful consequences, including the unnecessary surgical and medical interventions on intersexed bodies and the negative psychological effects of gender stereotypes. Anne Fausto-Sterling’s book, Sexing the Body: Gender Politics and the Construction of Sexuality (2000), advances necessary progressive feminist perspectives on the subject. I employ critical discourse analysis comparing original scientific and academic discourses with the author’s translations. I argue that she questions binary sex development and assignment in ways that …

Fluid Limit And Stochastic Stability For A Genetic Mutation Model, Carlos M. Bajo Caraballo

Open Access Dissertations

We develop the continuous time version of the particle model studied in [13]. The evolution is described by a pure jump, continuous time Markov process on the space of words of length L with a size N alphabet. Words change randomly in search of a preferred state, here the vector zero. In genome population models, this is the genome presenting selection advantage [21]; in cancer development, it is a state of a damaged gene by deleterious mutations, and in epidemiological models the number of infected individuals in the population. In the last two models, the characters in the preferred word …

Genetic Moderation Of Pain And Fatigue Symptoms Resulting From The Mindfulness-Based Stress Reduction For Breast Cancer Program, Carissa Bea Alinat

USF Tampa Graduate Theses and Dissertations

Breast cancer survivors (BCS) account for the largest group of cancer survivors living in the United States and they often experience lingering physical symptoms that may affect quality of life, with fatigue and pain the most commonly reported. This genetic research study was conducted within a parent R01 study, with the purpose of exploring associations between genetic variants and fatigue and pain symptoms and the Mindfulness-Based Stress Reduction for Breast Cancer (MBSR(BC)) program. The aims of this study were to: 1) identify specific genotypes involved in fatigue and pain symptoms, and 2) explore whether single nucleotide polymorphism (SNP) rs1800795 in …

Genetic Basis For Elevated Rheumatic Heart Disease Susceptibility In Samoa, John Bowen Allen

Theses and Dissertations

Rheumatic heart disease is an inflammatory heart disease that affects millions of people around the world. Especially high rates of the disease can be found in Oceania, including the island nation of Samoa. Genetic studies of immune response genes have provided insight into a possible genetic link to increased susceptibility to rheumatic heart disease, including the genes that code for the toll-like receptor (TLR) protein family. One of the functions of TLR proteins is to recognize the presence of bacteria via identification of bacterial flagella. My evaluation of a Samoan family identified a variant in the TLR-5 gene that would …

5-Httlpr, Drd4 Gene, College Students’ Psychological Characters And Academic Performance, Weiyi Guo

Graduate Dissertations and Theses

The purpose of this study, “Genetic basis of Academic Performance,” is to evaluate associations between the 5-HTTLPR, the DRD4 gene, psychological characteristics including metacognition and depression, and academic performance as reflected in SAT scores. We discovered that participants with 2-repeat or 7-repeat alleles of the DRD4 gene have significantly lower scores on the Metacognition tests (P = 0.022) and two subclades of metacognition, “Cognitive Self-Consciousness” (P = 0.021) and “Need to Control Thoughts” (P = 0.040), as compared to those without 2-repeat or 7-repeat alleles. 7-repeat allele DRD4 gene carriers have significantly higher scores for alcohol use disorders than those …

Light-Induced Expression Of A Blue Coral Protein In An Industrial Fungus, Lindsay Tomczak

Honors Theses

The ultimate goal of this research project is to improve the growth and structural characteristics of an Ecovative Design LLC (Green Island, NY) production strain to produce commercial biomaterials for packaging. These biomaterials are produced from renewable resources and can be easily broken down after they fulfill their purpose, unlike the commonly used materials today (such as Styrofoam). In an effort to quantify the light-reactivity of the fungus, a codon-optimized DNA sequence coding a blue chromoprotein was introduced and utilized as a visual reporter gene. Transcriptional controlling sequences were identified from orthologs to specific light-regulated genes and were combined with …

The Moderation Effect Of Bdnf Genotype And Self-Reported Habitual Physical Activity Levels On Age Of Onset, Disease Progression, And Postural Instability In Parkinson's, Kyle Johnson, Danielle Salgo, Jessica Zorn

UNLV Theses, Dissertations, Professional Papers, and Capstones

Background and Purpose: Brain-derived neurotrophic factor (BDNF) levels have been reported to play an important role in driving neuroprotection in people with neurologic disorders, and levels of BDNF are known to increase in response to physical activity. Moreover, the level of BDNF produced is also affected by BDNF genotype. It is not known, however, whether one’s BDNF genotype interacts with physical activity throughout life to affect a neuroprotective response in people with Parkinson’s disease (PD). Therefore, the purpose of this study was to determine if BDNF genotype interacts with lifetime self-reported physical activity levels to affect disease severity and progression …

Characteristics Of Individuals Undergoing Panel Genetic Testing For Primary Brain Tumors, Sarah Azam

Dissertations & Theses (Open Access)

Background. Currently, there are no genetic testing guidelines for patients with a primary brain tumor (PBT). This population is largely understudied in terms of the family history, tumor grade, pathology, and their relation to genetic contribution. Our aim was to describe patient-specific characteristics and family histories across mutation-positive, negative, and variant of uncertain significance (VUS) cohorts based on cancer-panel genetic test results among patients with a PBT.

Methods. Subjects were referred for multi-gene panel testing between March 2012 and June 2016. Clinical data were ascertained from test requisition forms. The incidence of pathogenic mutations (including likely pathogenic) and VUS’s were …

On The Feasibility Of Using Genetic Algorithms To Optimize The Structure Of Small Multilayer Perceptrons, Nicholas Dinep-Schneider

Honors Theses

Artificial neural networks, which mimic the human brain's ability to learn from experiences, are increasingly being used to analyze complex datasets. However, proper structural configuration requires intuition, trial-and-error, and frequent human attention. This study investigates an automated alternative that uses a Darwinian evolutionary strategy to optimize the structure of a small-scale Modified National Institute of Standards and Technology (MNIST) image classification network. Using accuracy as a measure of fitness, it examines the effect of varying the amount each network learns prior to differential reproduction. The accuracy of optimized networks was significantly higher than random initial networks, being increased by up …

Genetic Testing Practices Of Genetic Counselors, Geneticists, And Pediatric Neurologists With Regard To Childhood-Onset Neurogenetic Conditions, Sara Wofford

Dissertations & Theses (Open Access)

Identifying genetic diagnoses for neurological conditions with a considerable hereditary component, such as autism spectrum disorder (ASD), intellectual disability, and epilepsy, is critical to providing proper medical management for these patients and their families. However, many patients with these conditions are not tested appropriately or receive no genetic testing at all. The current study was designed to characterize the genetic testing practices of the providers most likely to evaluate or order genetic testing for these patients: pediatric neurologists, geneticists, and genetic counselors. The study noted significant variance between the testing strategies selected by pediatric neurologists compared to those of geneticists …

Regional Distribution, Non-Invasive Detection, And Genetic Diversity Of The Black-Spotted Newt (Notophthalmus Meridionalis), Evan A. Bare

Theses and Dissertations

The Black-spotted Newt (Notophthalmus meridionalis ) is one of three a salamander species native to Gulf Coast prairies of Texas and Mexico, with respective state and federal protections. This species has been neglected by the scientific community despite concerns of dramatic population declines and a globally endangered status, with the most recent work being conducted in the early 1990’s going unpublished. This study presents the most recent examination of the species providing probabilistic distribution maps, descriptions of three novel populations, assessments of surveying techniques, and the first known examination of intrapopulation genetics including the first documented genetic examination of …

Regulation Of The Tubulin Homolog Ftsz In Escherichia Coli, Monika S. Buczek

Dissertations, Theses, and Capstone Projects

Escherichia coli is a well-known pathogen, and importantly, a widely used model organism in all fields of biological sciences for cloning, protein purification, and as a model for Gram-negative bacterial species. And yet, researchers do not fully understand how this bacterium replicates and divides. Every year additional division proteins are discovered, which adds complexity to how we understand E. coli undergoes cell division. Due to their specific roles in cytokinesis, some of these proteins may be potential targets for development of antibacterials or bacteriostatics, which are much needed for fighting the current global antibacterial deficit. My thesis work focuses on …

Does Family Communication Matter? Exploring Knowledge Of Breast Cancer Genetics In Cancer Families, Sarah Harmon Davis

Theses and Dissertations

Purpose: Knowledge of breast cancer genetics is critical for those at increased risk whomust make decisions about breast cancer screening options. The purpose of this study was toexplore cognitive and emotional variables that might influence knowledge of breast cancergenetics.Methods: This descriptive, exploratory study analyzed theory-based relationships amongvariables related to knowledge of breast cancer genetics in cancer families. Participants includedfirst-degree relatives of women with breast cancer who had received genetic counseling andtesting; participants themselves did not have breast cancer and had not received geneticcounseling or testing. Data were collected by telephone interview and survey. Variables analyzedinclude numeracy, health literacy, cancer-related distress, …