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Unseen Science: Modern Discoveries Too Far Away Or Tiny For Human Eyes, Lucy Huang

Capstones

As science has progressed, scientists have realized that evidence goes beyond the realms of physical sight. Whether it is too small or difficult to find, scientists have developed different ways to get around this problem. We see this in cancer genomics and in extrasolar planetary research. Scientists use what they know and what they measure to validate their work.

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Phylogenetics Of Euthamia (Asteraceae: Astereae) And Asteromyia Euthamiae (Cecidomyiidae: Alycaulini), Marisa Szubryt

Honors Theses

No abstract provided.

Community Structure And Zonation Of Antarctic Benthic Invertebrates: Using A Remotely Operated Vehicle Under Ice To Define Biological Patterns, Dorota Szuta

Master's Theses

The Ross Sea, Antarctica is a deep bay of the Southern Ocean that exhibits seasonal sea ice and is adjacent to a permanent ice shelf overlying seawater. In 2008 and 2009, imagery of the seafloor under the McMurdo Ice Shelf and under the seasonal ice in the Ross Sea was collected via remotely operated vehicle (ROV) at depths to 300 m. Distinct differences in Antarctic benthic communities were observed over multiple environmental gradients. Species abundance typically exhibited a unimodal distribution with depth with mid-depth peaks, reflecting a food limitation at the deep end and potentially ice disturbance on the shallow …

A Teachable Unit On Soft Corals With Instructional Method Variances, Emily Breech

Honors Projects

The soft coral Sinularia flexibilis contains an aqueous alcohol extract that has shown antineoplastic activity against lymphocytic leukemia (Weinheimer and Matson, 1977). It also has other biological properties that work as antimicrobials, anti-inflammatory agents, and cytotoxicity activities (Kamel and Slattery, 2005). There is much research on the effects of flow rate on growth and morphology (Khalesi, Beeftink, & Wijffels, 2007) and the effects of light-dependency on growth rate (Khalesi, Beeftink, & Wijffels, 2009). The purpose of this student project was to further research on S. flexibilis, as well as incorporate that research into evaluating the effects of lecture-based teaching …

The Characterization Of Alpha-Carbonic Anhydrases Through Mutagenesis And Subcellular Localization In Arabidopsis Thaliana, Lance E. Pounds

LSU Master's Theses

Carbonic anhydrases (CAs) are zinc metalloenzymes which catalyze the interconversion of carbon dioxide and bicarbonate. It is hypothesized that CA activity facilitates CO₂ flux in leaf mesophyll cells to maintain optimal rates of photosynthesis or to maintain a pool of inorganic carbon for other metabolic processes. There are three classes of CAs (α, β, and γ) in the model plant Arabidopsis thaliana. The βCAs and γCAs have been well studied, but the αCAs have received relatively little study. There are eight αCA genes in Arabidopsis. This study investigates which αCAs are expressed in shoots, their tissue-specific expression, their …

Uncovering Tasselsheath3. A Genomic And Phenotypic Analysis Of A Maize Floral Mutant., Thompson Zhang

Masters Theses

In the modern era, maize has become the most successful crop grown in the United States. According to the USDA over 90 million acres of land are planted to corn and 96.2% of the U.S feed grain production is made up of the cereal. Part of the success of maize is due to its floral architecture, and its pollination technique in which the flower opens, exposing stamens containing pollen into the air. A unique organ called the lodicule functions as a release mechanism, forcing the flower to open. Lodicules from grasses and eudicot petals are homologous, yet there is little …

Life Begins, Yuju Yen

Theses

My work reveals the world I see, the world I feel, and the world I imagine. The story I want to tell in my thesis is about every stunning moment when a life begins. While the sculptures and jewelry in my thesis body of work differ in scale, they are all singing the same theme: when a life starts to grow and when a flower blooms.

The forms of my thesis work are inspired from various natural lives, such as virus, fungi, jellyfish, bacteria, blooming flowers, and sea creatures. They are vividly waving, rotating, spinning their tentacles, antennas, and microfibers …

Contemporary Morphological Evolution In A Lacustrine Population Of Threespine Stickleback, Rose Watts

Biology

In the face of rapidly changing environments, some populations go through contemporary evolution in response to altered selective pressures. In this situation, changes in morphology and behavior can be observed to monitor phenotypic transitions. In adaptive radiations such as the threespine stickleback, Gasterosteus aculeatus, species complex, both re-expression of ancestral phenotypes and the expression of novel phenotypes have been detected. I monitored a population of threespine stickleback in Lynne Lake, Alaska, which is undergoing rapid environmental changes due in part to an increase in primary productivity in the lake. Changes have been documented in the body size and foraging behavior …

Quantification Of Phenotypic Variation In Populations Of Newfoundland Threespine Stickleback (Gasterosteus Aculeatus), Nathan R. Biedak

Biology

Phenotypic variation in morphology driven by ecological factors has been well characterized in Alaskan and British Columbian populations of threespine stickleback, with studies demonstrating that the variation is extensive in a wide variety of anti-predator traits and body shape. In contrast, the limited published studies of stickleback in eastern North America suggest that there is relatively little among-population phenotypic variation in anti-predatory armor and body shape. Recent samples obtained from 30 populations of stickleback in Newfoundland, from a wide array of habitats, indicate that the earlier suggestion of relatively limited phenotypic diversity may need to be reevaluated. Using geomorphic morphometrics …

A Functional Analysis Of Drosophila Plcgamma And The Screening For A Novel Inhibitor, Michelle K. Latino

Biology

The epidermal growth factor receptor, EGFR, pathway is a highly conserved and complicated pathway required for cell growth, proliferation, migration, and proper regulation of the pathway is required for normal development (reviewed by Fischer et al., 2003). Due to the pathways role in development, the EGFR pathway is often implicated with various diseases including cancer. The EGFR pathway is regulated by various proteins including by PLCgamma which works to regulate the pathway by promoting motility, adhesion, and differentiation (Murillo-Maldonado et al., 2011; Butchar et al., 2012; Shepard et al., 2006). Because of the role PLCgamma plays in cell proliferation, it …

Evolution Of Nervous System Centralization: Role Of Bmp Signaling In Neural Fate And Dorsal-Ventral Axis Specification In Capitella Teleta, Christie Joyce

Biology

Bone Morphogenetic Protein (BMP) signaling plays an important role in establishing the dorsal-ventral (D-V) axis across Bilateria. BMP signaling has also been implicated in neural fate specification in several bilaterians that possess centralized nervous systems. The classic examples are found in vertebrates and Drosophila melanogaster, where opposing gradients of BMP2/4/Dpp and Chordin/Sog specify the D-V axis and the nervous system arises on the Chordin/Sog side of the early embryo due to the anti-neural role of BMPs. These similarities have been used as support for the hypothesis that the bilaterian ancestor possessed a centralized nervous system and that BMP signaling delimited …

The Effect Of Substrate Unevenness And Irregularity On Locomotor Performance And Kinematics Of Curly-Tailed Lizards, Marian E. Crockett

Biology

Uneven surfaces are ubiquitous in nature and animals must frequently move across them, yet their effects on locomotion are not well understood. I studied how surface unevenness and irregularity of substrates made of large particles affect the locomotor performance and kinematics of the terrestrial generalist sprinter, the Northern Curly-Tailed Lizard (Leiocephalus carinatus). I compared a flat surface (even), three sizes of spheres (regular but uneven surfaces), and three sizes of natural rocks (irregular and uneven surfaces). As the size of the particles increased, the unevenness of the surface increased. The lizards ran at similar speeds on all substrates, but compensated …

Preliminary Analyses Of The Diversity Of Soil Microbes On The Campus Of The University Of The Incarnate Word, Viridiana Wheeler

Theses & Dissertations

Soil samples were examined outside Bonilla Science Hall on the campus of the University of the Incarnate Word in San Antonio, Texas, at three time points between the calendar years 2016 and 2017. These time points correspond to Fall, Winter, and Spring. Samples were taken at two topsoil depths, 1 cm and 4 cm, to determine if there is a difference in bacterial load or distribution across the time frame examined.

Soil samples were diluted and plated on nutrient agar plates in order to identify unique colony morphologies. A total of 132 distinct morphological isolates were identified and sequenced. Of …

Oxidative Quenching Of Photoexcited Ru(Ii)-Bipyridine Complexes By Oxygen, Danielle Rebecca Latham

Undergraduate Honors Theses

An experimental approach was taken in determining the quantum yield of Ruthenium(II) with Oxygen using two different Ru complexes. This reaction results in Ruthenium(III) and O2-. The Coulombic interactions caused by a carboxylate functional was found to increase the yield of charge separation. This was done using a diode to measure the intensity of the completed reaction over a certain time frame. The intensities were turned into concentrations. The concentration over time was used to determine the quantum yield. This information is useful in creating more efficient light emitting diodes.

Pore Water Salinity Drives Brackish Vegetation Change In Salt Marsh Tidal Restoration, Shri Andrea L. Verrill Ms

All Student Scholarship

Salt marsh vegetation plays an important role in building and maintaining elevation on the marsh surface. For marshes to persist as sea-level-rise (SLR) occurs, the marsh accretion process must increase elevation at a rate greater than or equal to the rate of SLR. If salt marshes do not maintain elevation above sea level, salt marshes will drown, and associated benefits to humans, fish, and wildlife, and their contribution to the health of estuarine and marine ecosystems will be lost. Salt tolerant plant species are adapted to frequent and prolonged periods of inundation with seawater and typically maintain elevation where they …

Development Of Novel Bio- And Heterogeneous Catalysts For The Production Of Bio- And Green Diesel, Ye Deng

Doctoral Dissertations

Diminishing petroleum reserves and increasing environmental awareness has led to an urgent need to develop alternative fuels, such as biodiesel. Enzymatic trans/-esterification of waste cooking oils with a lipase catalyst is a promising environmentally-friendly process to produce biodiesel, compared to the current industrial chemical process. Despite several advantages, there are a few technical and economical obstacles that limit this process, such as insufficient availability of large quantities of inexpensive lipase suitable for catalysis, and bad performance at low temperatures due to biodiesel’s low cetane number. These limitations are addressed in this dissertation using genetic engineering of plants to produce the …

Genomic Evaluation Of Male Reproductive Adaptations And Responses To Dehydration In Peromyscus Eremicus (Cactus Mouse), Lauren Kordonowy

Doctoral Dissertations

Research elucidating the genetic architecture of physiological mechanisms enabling survival and reproduction in extreme environments is becoming prominent in evolutionary biology. The desert, in particular, poses numerous challenges for its endemic species, and mammals (and often, rodents) have been the focus for survival adaptations pertaining to water-limitation. However, desert rodent adaptation research has focused predominantly on survival, while potential physiological reproductive adaptations to dehydration have received less attention, aside from research evaluating water as reproductive cue. The fact that we do not know the physiological mechanisms enabling reproduction during dehydration is surprising, as desert rodents must possess adaptations to successfully …

Age And Sex-Specific Effect Of Caloric Restriction On Circadian Clock And Longevity-Associated Gene Expression, Arten Andreyevich Astafev

ETD Archive

The rhythms in the expression of circadian clock genes are affected by calorie restriction (CR), a dietary paradigm known to increase lifespan. In our current study, we show that circadian rhythms are influenced by sex and the effects of CR are different between males and females. In particular, we found a group of clock genes which showed a sex-dependent difference in expression, as well as in response to CR (Rev-Erb α, Ror γ and both Cryptochromes: Cry1 and Cry2 genes). Two clock genes showed no difference in expression but their response to CR showed sexual dimorphism (Ror α and Rev-Erb …

Dynamics And Clustering In Locust Hopper Bands, Jialun Zhang

HMC Senior Theses

In recent years, technological advances in animal tracking have renewed interests in collective animal behavior, and in particular, locust swarms. These swarms pose a major threat to agriculture in northern Africa, the Middle East, and other regions. In their early life stages, locusts move in hopper bands, which are huge aggregations traveling on the ground. Our main goal is to understand the underlying mechanisms for the emergence and organization of these bands. We construct an agent-based model that tracks individual locusts and a continuum model that tracks the evolution of locust density. Both these models are motivated by experimental observations …

Auranofin Targets Thioredoxin Reductases In Trichomonas Vaginalis, Jose Jauregui

University of the Pacific Theses and Dissertations

Trichomonas vaginalis is an anaerobic, parasitic protozoan, responsible for trichomoniasis, the world’s most common, non-viral sexually transmitted infection. Lacking many of the defenses present in other organisms to combat oxidative stress, Trichomonas vaginalis relies extensively on the thioredoxin system—NADPH, thioredoxin reductase, and thioredoxin—as a means to protect against exposure to excess oxygen. Current trichomoniasis treatment relies exclusively on the 5-nitroimidazole drugs, but fear of drug-resistant strains and allergic reactions to 5-nitroimidazole treatment necessitate the discovery of a new treatment method for trichomoniasis. Previous research has shown that auranofin, an FDA-approved drug, was effective at inhibiting activity of one of Trichomonas …

Studies On Development In Euphilomedes Ostracods: Embryology, Nervous System Development, And The Genetics Of Sexually Dimorphic Eye Development, Kristina Koyama

University of the Pacific Theses and Dissertations

Model organism studies have been fundamental in understanding evolutionary and developmental biology. However, non-model organisms present opportunities to study unique characteristics and as comparisons to model organisms, leading us toward broader and more relevant perspectives on diversity. The Euphilomedes genus of ostracods is an example of a non-model group with potential for evolutionary and developmental studies.

Ostracoda is an ancient, basally branching lineage of Crustaceans with a complete and prodigious fossil record. Despite the group’s promise for evolutionary studies, much remains unknown about the basic biology of this clade. There are a limited number of embryogenesis studies in Ostracoda; here, …

Evaluation Of Oyster Shell Enhancement On Western Snowy Plover Breeding Success, Karine Tokatlian

Master's Theses

The Pacific Coast population of the western snowy plover (Charadrius nivosus nivosus; plover) is listed as a federally threatened species under the U.S. Endangered Species Act due to poor reproductive success from anthropogenic habitat loss, and high predation pressure. Plovers in the South San Francisco Bay use dry salt evaporation ponds, and wildlife-managed ponds, to breed and winter. However, the South Bay Salt Pond Restoration Project aims to restore up to 6,110 hectares of this habitat back to native tidal marsh, which will force plovers to breed in smaller areas and in higher densities. In order to recover plovers in …

Vector Competence Of Aedes Sierrensis And Culex Pipiens Complex (Diptera: Culicidae) For Dirofilaria Immitis (Spirurida: Onchocercidae) In Northern California, Jeffrey Allan Kurosaka

University of the Pacific Theses and Dissertations

Dirofilaria immitis Leidy (dog heartworm) is a life-threatening parasite transmitted by mosquitoes to domestic dogs. Endemic in the eastern United States, cases have become more prevalent over the last few decades. While prevalence in California is generally low, Lake and San Joaquin Counties have reported rates comparable to the East Coast at 3.73% and 0.71%(CAPC 2017), respectively. Aedes sierrensis is thought to be responsible for transmission in California, but in some cases, it exists in inadequate quantities and temporal ranges to explain parasite activity. Based on Huang et al. (2013) and Tran (2016), bloodfeeding patterns, and other vector criteria, Culex …

A Novel System For Detection Of Dna Double Strand Breaks And Repair In Human Cells, Todd Warren Lewis

Browse all Theses and Dissertations

Imperative to genomic stability is the ability of the cell to repair damaged DNA which can occur from numerous endogenous byproducts of metabolism or exogenous components from the environment. The Fanconi anemia pathway is a DNA repair mechanism used by human cells to resolve multiple forms of DNA damage including interstrand crosslinks (ICL). Fanconi anemia (FA) is an autosomal recessive inherited disorder characterized by genome instability, developmental abnormalities, cancer predisposition and bone marrow failure. FA is attributed to a mutations in at least 18 genes (FANCA-FANCT) that play a concerted role in DNA repair. FANCT is the latest discovery in …

Determinants Of Host Use In Tachinid Parasitoids (Diptera: Tachinidae) Of Stink Bugs (Hemiptera: Pentatomidae) In Southwest Ohio, Matthew W. Duncan

Browse all Theses and Dissertations

Tachinid parasitoids in the subfamily Phasiinae are important natural enemies of heteropteran bugs. Host location by these flies occurs via antennal reception to the pheromones of their hosts; however little is known regarding the mechanisms which underlie host selection. Halyomorpha halys, the invasive brown marmorated stink bug, represents a potential novel host species in North America. This study was conducted to determine the suitability of H. halys as a host for phasiine species, and to assess cues used in host selection by the species Gymnoclytia occidua. Field attraction to pentatomid pheromones by both phasiines and pentatomids in Southwest Ohio were …

The Effect Of Aging On The Blood Brain Barrier Permeability And Response To Fluoxetine Enantiomers, Ethar Arkan

Browse all Theses and Dissertations

We tested the effect of the fluoxetine enantiomers (S-fluoxetine and R-fluoxetine) versus Prozac (50:50 ratio of R- and S- fluoxetine enantiomers) and/or control on blood brain barrier (BBB) permeability in different brain regions in both male and female rats. The rats consumed orally the drug (5 mg/kg) or vehicle for a total of three days, then were injected with sterile Evans blue dye ip, at least 12 hours before euthanasia.. We see significant regional brain differences in BBB permeability (hippocampus has tighter BBB), significant differences based on the age of the animals (young rats show enhanced permeability in lower brain …

Gutted, Philip Alexander Drumm

Electronic Theses and Dissertations

Gutted is a collection of poems that re-evaluate the way in which we look at the human body. These poems look to fuse the taxonomical properties of an anatomy book with the subjectivity of poetry in order to imagine physical manifestations of metaphysical events in the body. Included in these works are anatomical drawings that create visual representations of their accompanying poetic content. These drawings are conceptualized based upon how each part of the body is described rather than what they look like in reality. Many of the poems in this manuscript call upon the history of medicine for their …

The Effect Of Arginine Vasotocin On Cryoprotectant Production During Freezing In The Pacific Chorus Frog, Pseudacris Regilla, Ben Ingels

All Master's Theses

Freeze Tolerance is an adaptation seen amongst several species of amphibians as a way of surviving during wintering months. Use of cryoprotectants is seen in all freeze tolerant species (glucose/glycerol). Synthesis and transportation mechanisms that regulate this process are not yet fully understood. Arginine vasotocin is a neurohypophyseal peptide known to have glycogenolytic effects as well as regulate several key processes involved in fluid and solute transportation. Although one prior study suggested that AVT is not involved in responses to freezing, I measured AVT levels during freezing and injected an AVT antagonist to see if it would affect the production …

Effects Of The Biomedical Bleeding Process On The Behavior And Physiology Of The American Horseshoe Crab, Limulus Polyphemus, Meghan Owings

Master's Theses and Capstones

Horseshoe crabs are harvested by the biomedical industry in order to create Limulus Amebocyte Lysate (LAL) to test medical devices, vaccines and pharmaceutical drugs for pathogenic gram-negative bacteria. Previous studies of the impacts of the biomedical bleeding process on horseshoe crabs have primarily focused on mortality rates and sublethal impacts, using animals held in the laboratory. Therefore, the first goal of this project was to determine the effects of the bleeding process on horseshoe crab behavior once they are released back into their natural environment. In addition, previous studies have typically only investigated the impacts of the full bleeding procedure, …

The Impact Of Socs1 And Socs3 Peptide Mimetics On Rho And Cdc42 Proteins Expression, F-Actin Cytoskeleton Rearrangements, And Cytokines Production Of Uninfected And Hsv-1 Infected M1 And M2 Raw 264.7 Murine Macrophages, Maha A. Elwardany

Browse all Theses and Dissertations

The immune system plays an essential role in the pathogenesis of HSV-1 during the lytic phase of the disease, orchestrating the inflammatory response, retaining the virus in its latent phase and preventing the recurrence of HSV-1 infection. Macrophages display a vital role in the innate and adaptive immune responses during multiple phases of HSV-1 infection. Polarized macrophages are categorized into two distinct classes with diverse functions. The classically activated M1 can engulf and destroy the microbial agents, produce proinflammatory cytokines, and participate in the pathogenesis of many inflammatory diseases. The alternatively activated M2 induces anti-inflammatory mediators and stimulates tissue remodeling …