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A Specialized Citric Acid Cycle Requiring Succinyl-Coenzyme A (Coa):Acetate Coa-Transferase (Aarc) Confers Acetic Acid Resistance On The Acidophile Acetobacter Aceti, Elwood Mullins
All Theses and Dissertations (ETDs)
The characteristic ability of acetic acid bacteria to aerobically oxidize ethanol to acetic acid has been harnessed for millennia to produce vinegar. Acetic acid permeates cell membranes at low pH and generally inhibits bacterial growth at low millimolar concentrations. The strains used for vinegar production, however, thrive in near-molar concentrations. This remarkable resistance results from the combined contributions of several molecular mechanisms. This study examines the inherent acid stability of proteins from the industrial vinegar-production strain Acetobacter aceti 1023 and the process by which cytoplasmic acetic acid is "overoxidized" to carbon dioxide. Acetate overoxidation in A. aceti strain 1023 is …
Investigation Of The Structure Of Alkyne Self-Assembled Monolayers On Gold And The Development Of Matrix-Enhanced Secondary Ion Mass Spectrometry Methods, Jennifer Dertinger
Investigation Of The Structure Of Alkyne Self-Assembled Monolayers On Gold And The Development Of Matrix-Enhanced Secondary Ion Mass Spectrometry Methods, Jennifer Dertinger
All Theses and Dissertations (ETDs)
Characterization of thin films is critical to the understanding of many technological and biological processes. The focus of this dissertation is to develop methods to characterize very low concentration species present on surfaces. A reflection adsorption infrared spectroscopy: RAIRS) system was constructed and tested. The instrument comprises an Fourier transform infrared: FTIR) spectrometer, an optical pathway and a vacuum chamber. The RAIRS system is designed to investigate in situ the interaction of vapor-deposited metals and gases, such as CVD precursors, with organic thin films, and so a vacuum chamber is required. To accommodate the vacuum chamber, an external IR optical …