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Investigation Of Biodegradation Processes In Solid Waste Landfills, Philip A. Colborn Dec 1997

Investigation Of Biodegradation Processes In Solid Waste Landfills, Philip A. Colborn

Theses and Dissertations

Greater demands on landfill capacity, stricter regulations intended to minimize landfill environmental impacts, and the economic potential associated with landfill operations have shifted the emphasis of landfill disposal toward methods concerned with the long term performance and capacity of landfills. Two opposing philosophies have emerged in constructing and managing landfills: the dry tomb and the wet cell. The key to managing a landfill from the wet cell viewpoint is first understanding the biodegradation processes occurring within the landfill. This thesis attempts to determine the fundamental processes responsible for the degradation of solid waste by employing a system dynamics approach. A …


Endogenous Alkylglycerol Functions As A Mediator Of Protein Kinase C Activity And Cell Proliferation, Fritz G. Buchanan May 1997

Endogenous Alkylglycerol Functions As A Mediator Of Protein Kinase C Activity And Cell Proliferation, Fritz G. Buchanan

Electronic Theses and Dissertations

To explore the possibility that 1-O-alkyl-sn-glycerol (alkylglycerol) may serve a regulatory role in the control of cell proliferation or PKC activity, we examined the ability of alkylglycerol to influence PKC activity and subcellular distribution as well as the ability of alkylglycerol to effect cell proliferation. MDCK cells grown to confluence show a loss of PKC activity associated with the membrane, as reported in fibroblasts. Preconfluent cultures of MDCK cells have a high level of PKC activity associated with the membrane. However, treatment of preconfluent cultures with alkylglycerol causes a reduction of PKC activity. A similar inhibition was observed with alkylglycerol …


Binding Of Cobalt (Iii) To Nucleic Acids Via Reaction With [Co(Nh3)5(Oh2)]3+, David M. Calderone May 1997

Binding Of Cobalt (Iii) To Nucleic Acids Via Reaction With [Co(Nh3)5(Oh2)]3+, David M. Calderone

Seton Hall University Dissertations and Theses (ETDs)

No abstract provided.


Reductive Precipitation Of Uranium By Free And Immobilized Cells And Cell Fractions Of Facultative And Anaerobic Bacteria., Boyce Lamont Clark Jan 1997

Reductive Precipitation Of Uranium By Free And Immobilized Cells And Cell Fractions Of Facultative And Anaerobic Bacteria., Boyce Lamont Clark

LSU Historical Dissertations and Theses

This study examines the interaction between two genera of bacteria and the biochemical reduction of uranium. The bacteria studied are the obligate anaerobe, Desulfovibrio desulfuricans, and the facultative aerobe, Escherichia coli. Preliminary experiments established the pH, cell concentration, substrate concentration, and type of substrate that favored reduction of hexavalent uranium in aqueous solutions. Uranium reduction is most complete at pH 7 and 9, with cell concentrations of at least 33.5 mg/ml (dry cell weight). Whole cells of D. desulfuricans were found to reduce uranium in the presence of lactate and hydrogen whereas in E. coli, reduction was achieved only in …


Cloning, Sequencing And Regulation Of The Rhodobacter Capsulatus Hemh Gene., Ekaterina Vladislavova Kanazireva Jan 1997

Cloning, Sequencing And Regulation Of The Rhodobacter Capsulatus Hemh Gene., Ekaterina Vladislavova Kanazireva

LSU Historical Dissertations and Theses

Rhodobacter capsulatus, a purple non-sulfur photosynthetic bacterium, synthesizes four tetrapyrrole end products: heme, bacteriochlorophyll, siroheme and vitamin B$\sb{12}$ via the common tetrapyrrole biosynthetic pathway. The biosynthesis of tetrapyrroles in R. capsulatus is a complex and precisely controlled process which has been under extensive investigation. In order to study the regulation of tetrapyrrole biosynthesis, the R. capsulatus hemH gene was cloned by complementation of an E. coli $\Delta hemH$ mutant and sequenced. The enzyme ferrochelatase, encoded by the hemH gene, catalyzes the insertion of ferrous iron into protoporphyrin IX, thus producing heme. The consensus ferrochelatase signature sequence was found in the …