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Hsp 72 In Rat Skeletal Muscle: Fibre Type Differences And Effect Of Exercise, Marius Locke
Hsp 72 In Rat Skeletal Muscle: Fibre Type Differences And Effect Of Exercise, Marius Locke
Digitized Theses
Western blot analyses of SDS-PAGE separated proteins from various hindlimb muscles from non-exercised control animals probed with an HSP 72 monoclonal antibody, demonstrated the constitutive expression of HSP 72 in rat muscles comprised primarily of Type I muscle fibres (soleus), but not in muscles comprised primarily of Type IIB/X fibres (white gastrocnemius-WG). In muscles of mixed fibre type, (plantaris and red gastrocnemius-RG) HSP 72 content was proportional to the percentage of Type I fibres.;Immunolocalization of HSP 72 in cross-sections from the soleus muscle demonstrated that both Type I and Type IIA fibres expressed HSP 72, but Type I fibres expressed …
Characterization Of Invariant Membrane Proteins Of Trypanosoma (Duttonella) Vivax, Barbara Anne Burleigh
Characterization Of Invariant Membrane Proteins Of Trypanosoma (Duttonella) Vivax, Barbara Anne Burleigh
Digitized Theses
Monoclonal antibodies (mAbs), raised against whole, fixed, uncoated, culture forms of Trypanosoma (Duttonella) vivax, were used to identify two invariant membrane proteins of this protozoan parasite. Since non-variant membrane proteins of the cell surface, flagellar pocket and endocytic pathway are potential targets for the control of trypanosomiasis of livestock by immunization, the identification and characterization of invariant membrane proteins is a necessary preliminary step.;A 65 kDA invariant membrane glycoprotein (gp65), identified using mAb 4E1, was the main focus of this study. Immunolocalization studies using the monoclonal antibody (mAb 4E1) for immunofluorescence staining and immunoelectron microscopy, demonstrated that the 65 kDa …
Genomic Characterization Of Steroid Hormone-Binding Globulins, D Alan Underhill
Genomic Characterization Of Steroid Hormone-Binding Globulins, D Alan Underhill
Digitized Theses
Sex-hormone binding globulin (SHBG) and corticosteroid binding globulin (CBG) are the major plasma transport proteins for sex-steroids and glucocorticoids, respectively, and may impact directly upon steroid hormone bioavailability to target cells. Androgen binding protein (ABP) is closely related to SHBG and is believed to maintain an androgenic environment for sperm maturation. The isolation of SHBG-related cDNAs from human testis and a corresponding transcription unit indicates that SHBG and ABP are encoded by the same gene. In addition, the SHBG/ABP gene gives rise to two novel testicular transcripts through a process of alternative splicing. The putative proteins encoded by these transcripts …
Isolation And Characterization Of A Genetic Locus Involved In Growth And Pathogenicity On Tomato Plants By Pseudomonas Syringae Pv Tomato, Dennis Paul Jackson
Isolation And Characterization Of A Genetic Locus Involved In Growth And Pathogenicity On Tomato Plants By Pseudomonas Syringae Pv Tomato, Dennis Paul Jackson
Digitized Theses
Pseudomonas syringae pv. tomato is the causal agent of bacterial speck on tomato. An essential feature of this organism, in the pathogenic process, is its ability to enter the intercellular spaces of its host and use the available plant substrates for growth. Tomato leaves contain quantities of carbon energy in the form of carboxylates. A Tn5 mutant, DC3481, cannot utilize carboxylates as a source of carbon energy for growth (CA{dollar}\sp-{dollar}) and is nonpathogenic (Path{dollar}\sp-{dollar}) on tomato. The focus of this thesis was to utilize DC3481 to isolate and characterize P. syringae pv. tomato DNA sequences involved with growth and pathogenicity …
The Reproductive Ecology Of Convict Cichlids In Costa Rica, Brian Douglas Wisenden
The Reproductive Ecology Of Convict Cichlids In Costa Rica, Brian Douglas Wisenden
Digitized Theses
Convict cichlids (Cichlasoma nigrofasciatum) form monogamous pair bonds and have cooperative biparental care of their eggs, larvae and fry for up to six weeks. This fish is widely used in laboratory studies as a model of biparental care. However, very little is known of its habits in its natural habitat. I studied four natural populations of convict cichlids, in 'pool' and 'stream' habitat, in Costa Rica during the long dry season, December to June in 1990 and 1991. The number of pairs guarding broods increased from December to a peak in mid-March, then rapidly declined. Twenty-eight percent of 175 males …
Connexin Gene Expression And The Control Of Gap Junction Assembly In Preimplantation Development, Gunnar Valdimarsson
Connexin Gene Expression And The Control Of Gap Junction Assembly In Preimplantation Development, Gunnar Valdimarsson
Digitized Theses
Gap junctional communication in preimplantation mouse embryos was explored by investigating: (1) the expression pattern of the connexin (Cx: gap junction protein) multigene family in maturing mouse oocytes and preimplantation embryos; (2) the relative role of Cx43 in gap junctional communication in embryos; and (3) the control of the acquisition of gap junctional coupling.;Both Cx43 and Cx32 transcripts were detected in maturing oocytes but these transcripts declined to undetectable levels shortly after ovulation. Cx43 transcripts were detected again at the 4-cell stage and accumulated steadily thereafter to reach a maximum in blastocysts. Cx26 and Cx46 transcripts could not be detected …
Suppression Of Eae In The Lewis Rat By Treatment With Neuroantigen-Containing Liposomes, Colleen Sheila Stein
Suppression Of Eae In The Lewis Rat By Treatment With Neuroantigen-Containing Liposomes, Colleen Sheila Stein
Digitized Theses
Treatment of Lewis rats with intracardiac injections of neuroantigen-containing liposomes significantly reduced the clinical severity of spinal cord-induced experimental allergic/autoimmune encephalomyelitis (EAE). Liposomes containing myelin basic protein (MBP), galactocerebroside (GC), or MBP + GC were effective in this respect, while liposomes containing proteolipid protein were not. The best protection was afforded by liposomes composed of whole myelin, as these ameliorated both the acute and relapsing phases of the disease, with a concomitant reduction in CNS pathology.;Neuroantigen-specific proliferative responses of lymphnode cells (LNC) taken from liposome-treated rats were markedly attenuated in comparison to control LNC, while delayed type hypersensitivity (DTH) reactions …
Determinants Of Early Childbearing Among Young Female Adults In The Gambia, Momodou S. Jeng
Determinants Of Early Childbearing Among Young Female Adults In The Gambia, Momodou S. Jeng
Digitized Theses
This thesis examines the determinants of childbearing among young Gambian women aged 13 to 24 and ranks the respective components (or groups of variables) in order of importance. An empirical analysis is done using bivariate and multi-variate statistical techniques. Based on these analyses, it is found that the factors of the empirical model (combining the reference group theory and a Bongaarts-type framework) vary in their effect on the fertility of young women. The most important of the socioeconomic, demographic and cultural (SEDC) variables are educational attainment, employment status, looking for work, area of residence, age, number of siblings, rank of …
The Quantum And Tempo Of Childbearing In Iran, Before And After The Islamic Revolution, Mohammad Karim Mansoorian
The Quantum And Tempo Of Childbearing In Iran, Before And After The Islamic Revolution, Mohammad Karim Mansoorian
Digitized Theses
This study presents an analysis of demographic, socio-economic and environmental characteristics that may influence the quantum and timing of first, second and third births among Iranian women. This study focuses on the role that these variables played in influencing the fertility behaviour before, during and after the Islamic Revolution. The birth history data gathered by the 1988 Pars Province Growth Rate Survey-Iran are used in this study. An in-depth fertility questionnaire permits comparisons before and after the Islamic Revolution. The sample contains 2511 ever married women aged 15 years and above. Since these women have different marriage experience that can …
Neurotransmitter Interactions In The Substantia Nigra And In The Neostriatum Following Nigral Grafts, Ivar Marcelo Mendez
Neurotransmitter Interactions In The Substantia Nigra And In The Neostriatum Following Nigral Grafts, Ivar Marcelo Mendez
Digitized Theses
Direct synaptic contact between substance P (SP)-immunoreactive terminals and glutamic acid decarboxylase (GAD)-positive neurons was more often observed in the substantia nigra pars lateralis (SNL) than in the substantia nigra pars reticulata (SNR) and was rarely observed in the substantia nigra pars compacta (SNC). Substance P terminals also formed synapses with cell bodies and dendrites of tyrosine hydroxylase (TH)-positive, dopaminergic neurons in the SNC and SNR. Multiple SP terminals were often observed with symmetrical and, less frequently, asymmetrical synapses on individual TH-containing dendrites. Evidence of SP-containing terminals contacting both GABAergic and dopaminergic neurons in the substantia nigra suggests a direct …
Control Of Tonic Sympathetic Nerve Activity By The Brainstem In Rats, Kristen Hayes
Control Of Tonic Sympathetic Nerve Activity By The Brainstem In Rats, Kristen Hayes
Digitized Theses
Control of tonic firing of sympathetic nerves by the rostral ventrolateral medulla (RVLM) and the pontine reticular formation (PRF) was investigated. Electrical activity of renal, splenic, mesenteric and preganglionic greater splanchnic nerves and T{dollar}\sb{lcub}13{rcub}{dollar} white rami was recorded in artificially respired, urethane-anaesthetized rats. Glycine (1.0M;180nl) was microinjected into the RVLM to inhibit neuronal discharge and effects on these nerves were compared. To study descending inhibitory influences on these nerves, effects of RVLM blockade were compared to effects of cervical spinal cord transection. RVLM blockade caused greater decreases in discharge of renal ({dollar}-44\ \pm\ 3\%{dollar}) than splenic ({dollar}-31\ \pm\ 2\%{dollar}) nerves, …