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High Ethanol Concentration (77 G/L) Of Industrial Hemp Biomass Achieved Through Optimizing The Relationship Between Ethanol Yield/Concentration And Solid Loading, Jikai Zhao, Youjie Xu, Weiqun Wang, Jason Griffin, Donghai Wang Aug 2020

High Ethanol Concentration (77 G/L) Of Industrial Hemp Biomass Achieved Through Optimizing The Relationship Between Ethanol Yield/Concentration And Solid Loading, Jikai Zhao, Youjie Xu, Weiqun Wang, Jason Griffin, Donghai Wang

School of Earth, Environmental, and Marine Sciences Faculty Publications and Presentations

In this study, the relationships between ethanol yield/concentration and solid loading (6–21%) were investigated to enhance ethanol titer and avoid a random choice of solid loading for simultaneous saccharification and fermentation (SSF). Alkali-pretreated hemp biomass was used for SSF in four scenarios including Case I: 30 filter paper unit (FPU)-cellulase and 140 fungal xylanase unit (FXU)-hemicellulase/g-solid; Case II: 40 FPU-cellulase and 140 FXU-hemicellulase/g-solid; Case III: 30 FPU-cellulase and 140 FXU-hemicellulase/g-solid with 1% Tween80; and Case IV: 30 FPU-cellulase and 140 FXU-hemicellulase/g-solid with particle size reduction (<0.2 mm). Results showed that bioethanol yield and concentration had a negative linear (R2 = 0.76–0.93) and quadratic (R2 = 0.96–0.99) correlation with solid …


Solid-State Nmr Of Plant And Fungal Cell Walls: A Critical Review, Wancheng Zhao, Liyanage D. Fernando, Alex Kirui, Fabien Deligey, Tuo Wang Jun 2020

Solid-State Nmr Of Plant And Fungal Cell Walls: A Critical Review, Wancheng Zhao, Liyanage D. Fernando, Alex Kirui, Fabien Deligey, Tuo Wang

Faculty Publications

© 2020 Elsevier Inc. The cell walls of plants and microbes are a central source for bio-renewable energy and the major targets of antibiotics and antifungal agents. It is highly challenging to determine the molecular structure of complex carbohydrates, protein and lignin, and their supramolecular assembly in intact cell walls. This article selectively highlights the recent breakthroughs that employ 13C/15N solid-state NMR techniques to elucidate the architecture of fungal cell walls in Aspergillus fumigatus and the primary and secondary cell walls in a large variety of plant species such as Arabidopsis, Brachypodium, maize, and spruce. Built upon these pioneering studies, …