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United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
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Characterization Of A Higher Plant Herbicide-Resistant Phytoene Desaturase And Its Use As A Selectable Marker, R. S. Arias, Franck E. Dayan, Albrecht Michel, J’Lynn Howell, Brian E. Scheffler
Characterization Of A Higher Plant Herbicide-Resistant Phytoene Desaturase And Its Use As A Selectable Marker, R. S. Arias, Franck E. Dayan, Albrecht Michel, J’Lynn Howell, Brian E. Scheffler
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Three natural somatic mutations at codon 304 of the phytoene desaturase gene (pds) of Hydrilla verticillata (L. f. Royle) have been reported to provide resistance to the herbicide fluridone. We substituted the arginine 304 present in the wild-type H. verticillata phytoene desaturase (PDS) with all 19 other natural amino acids and tested PDS against fluridone. In in vitro assays, the threonine ( Thr), cysteine (Cys), alanine (Ala) and glutamine (Gln) mutations imparted the highest resistance to fluridone. Thr, the three natural mutations [Cys, serine (Ser), histidine (His)] and the wild-type PDS protein were tested in vitro against seven …