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Carriage Of Mobilizable Plasmid-Mediated \Beta-Lactamase Gene In Ampicillin-Resistant Escherichia Coli Strains With Origin Of Normal Fecal Flora, Osman Bi̇rol Özgümüş, İlknur Tosun, Faruk Aydi̇n, Ali̇ Osman Kiliç, Murat Ertürk

Turkish Journal of Medical Sciences

Aim: The aim of this study was to investigate the carriage of b-lactamase genes in ampicillin-resistant (Amp^r) Escherichia coli (E. coli) isolates from human normal fecal flora. Methods: Ten Amp^r E. coli strains isolated from the stool samples of 21 healthy persons with no antibiotic use during at least three months were screened for TEM-, SHV-, or OXA-type \beta-lactamase genes by polymerase chain reaction (PCR). The susceptibility of the strains to antibiotics was determined by disk diffusion method, and minimum inhibitory concentration (MIC) of ampicillin to the strains was determined by agar dilution method. Plasmid transfer assays were performed by …