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Analysis Of Bacterial Diversity And Biogeography At The Central Arizona-Phoenix Long Term Ecological Research (Cap Lter) Site, Brian Anthony Rash
Analysis Of Bacterial Diversity And Biogeography At The Central Arizona-Phoenix Long Term Ecological Research (Cap Lter) Site, Brian Anthony Rash
LSU Doctoral Dissertations
The limiting factor involved in past assessments of soil bacterial diversity when using culture-independent techniques has often been the lack of sampling and replication. As a result, analyses of community structural shifts across soil environments have lacked statistical power. In this study, 23 16S rRNA gene clone libraries consisting of over 11,000 clones were constructed from soils at the Central Arizona Phoenix Long Term Ecological Research (CAP LTER) site. Subsequent ARDRA fingerprinting and partial 16S rRNA gene sequencing allowed for a more robust investigation of various components that may explain any observed variations in bacterial species composition. The designated land …