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Novel Platforms For Large-Scale Adherent Culture Of Mammalian Cells, Ashkan Yekrangsafakar

LSU Doctoral Dissertations

With recent advances in biotechnology, there is a strong and urgent need for robust platforms to culture mammalian cells on a large scale to produce biopharmaceuticals. To this end, various bioreactors have been developed over the past decades, but their capacity and efficiency are often limited by insufficient mass transfer rate and excessive shear stress. In this work, multiple novel bioreactors for the large-scale adherent culture of anchorage-dependent cells were developed.

Hollow MicroCarriers (HMC) was developed as an alternative solution for the microcarrier-based culture system in a stirred-tank bioreactor. In the conventional microcarrier technique, cells are exposed to the harmful …