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Numerical Investigation Of Cryopreserved Zebrafish Sperm Cell Activation In Microchannels, Thomas Foster Scherr
Numerical Investigation Of Cryopreserved Zebrafish Sperm Cell Activation In Microchannels, Thomas Foster Scherr
LSU Doctoral Dissertations
This aim of this research project is to probe the activation process of zebrafish spermatozoa. Zebrafish are a model species for biological engineering applications, and the cryopreservation of their reproductive cells allows for inexpensive cataloging and maintenance of valuable biological material. Evaluation of cryopreservation protocols for aquatic sperm cells is typically accomplished by motility analysis after subjecting cells to a cryopreservation treatment. In zebrafish sperm cells, motility is initiated when cells come into contact with a hypo-osmotic environment. Subsequent activation analysis is currently done manually and brings with it an inherent difficulty and error. This process is slow and not …
The Effects Of Asymmetric Micro Ratchets On Pool Boiling Performance, Lance Austin Brumfield
The Effects Of Asymmetric Micro Ratchets On Pool Boiling Performance, Lance Austin Brumfield
LSU Doctoral Dissertations
Nucleate boiling is an attractive method for achieving high heat flux at low superheat temperatures. It is frequently used for industrial applications such as heat exchangers and is being considered to cool advanced central processing units (CPU) which produce heat fluxes on the order of 1 MW/m2 and are becoming increasingly less efficient to cool via forced conduction of air. The issue with implementing nucleate boiling as a cooling mechanism lies in the difficulty of quantifying the numerous and complex mechanisms which control the process. A comprehensive nucleate boiling model has yet to be formulated and will be required in …
Model-Based Tests Of Historical Demography And Species Delimitation In The Caribbean Coral Reef Sponge Callyspongia, Melissa Barrett Debiasse
Model-Based Tests Of Historical Demography And Species Delimitation In The Caribbean Coral Reef Sponge Callyspongia, Melissa Barrett Debiasse
LSU Doctoral Dissertations
Coral reefs are the most productive and species rich ecosystems in the ocean yet we lack knowledge about the distribution of genetic variation the within and among reef species, particularly for the sponges (Porifera). My dissertation describes how genetic variation at mitochondrial and nuclear genes is partitioned among and within species in the sponge genus Callyspongia. I compared patterns of genetic diversity and population subdivision in the mitochondrial and nuclear genomes of one species, C. vaginalis, in Florida (Chapter 2). Previous work revealed three divergent mitochondrial lineages, but nuclear alleles did not correspond to either mitochondrial clade or geography. Coalescent …