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LSU Doctoral Dissertations

Theses/Dissertations

2004

Chlamydomonas

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Molecular, Genetic And Physiological Characterization Of A Chlamydomonas Reinhardtii Insertional Mutant, James Edward,Iv Adams Jan 2004

Molecular, Genetic And Physiological Characterization Of A Chlamydomonas Reinhardtii Insertional Mutant, James Edward,Iv Adams

LSU Doctoral Dissertations

Photosynthetic microorganisms must acclimate to environmental conditions that may lead to photo-oxidative stress, such as low CO2 environments or high light intensities. Chlamydomonas reinhardtii, a unicellular, green alga with a Carbon Concentrating Mechanism (CCM), effectively accumulates inorganic carbon (Ci) to levels higher than external concentrations. The CCM concentrates inorganic carbon around Rubisco to increase the CO2 fixation efficiency of C. reinhardtii. Photorespiration, the water-water cycle, the xanthophyll cycle, and the CCM are adaptations that also prevent the over-reduction of photosystems and thus photoinhibition by dissipating the energy from the absorption of excess photons. In an effort …


Ctp: Phosphoethanolamine Cytidylytransferase And Dag: Cdp-Ethanolamine Ethanolaminephosphotransferase In The Cdp-Ethanolamine Pathway Of Chlamydomonas Reinhardtti, Wenyu Yang Jan 2004

Ctp: Phosphoethanolamine Cytidylytransferase And Dag: Cdp-Ethanolamine Ethanolaminephosphotransferase In The Cdp-Ethanolamine Pathway Of Chlamydomonas Reinhardtti, Wenyu Yang

LSU Doctoral Dissertations

Chlamydomonas reinhardtii Dangeard does not have two major phospholipids, PS and PC. This fact renders C. reinhardtii a desirable system for investigations of the PE biosynthetic pathway and its regulation independent of PC and PS biosynthesis. The cDNA coding for ECT protein in C. reinhardtii was cloned from a cDNA library. The ECT cDNA encodes a protein of 443 amino acid residues. The ECT protein in C. reinhardtii has a repetitive internal sequence in its N- and C-terminal halves. Each repeat half of the protein has a HXGH motif, a site considered to be in the catalytic domain. The protein …