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Carbonic Anhydrase And Carbonic Anhydrase Like Genes Of Chlamydomonas Reinhardtii, Mautusi Mitra
Carbonic Anhydrase And Carbonic Anhydrase Like Genes Of Chlamydomonas Reinhardtii, Mautusi Mitra
LSU Doctoral Dissertations
Carbonic anhydrase (CA) is a zinc containing metalloenzyme that catalyzes the reversible interconversion of CO2 and HCO3-. There are three evolutionarily unrelated CA families designated alpha, beta and gamma CA. Vertebrates have members of the alpha CA family, while higher plants, algae and cyanobacteria have members belonging to all three CA families. In the green alga, Chlamydomonas reinhardtii, five CAs have previously been identified including three alpha CAs and two beta CAs. This dissertation describes the identification and characterization of new CA genes from C. reinhardtii. Four new CA or CA like genes have been discovered including two beta CAs …
High Carbon Dioxide Requiring Mutants Of Chlamydomonas Reinhardtti, Steve Vincent Pollock
High Carbon Dioxide Requiring Mutants Of Chlamydomonas Reinhardtti, Steve Vincent Pollock
LSU Doctoral Dissertations
Chlamydomonas reinhardtii is a photosynthetic alga that has the ability to concentrate CO2 around Rubisco to achieve enhanced rates of photosynthesis in a low CO2 environment. This dissertation presents results obtained from the generation and analysis of four high CO2 requiring mutants of C. reinhardtii. The use of reverse genetics is a very powerful tool to dissect out the individual components of metabolic pathways. Two reverse genetics methods were utilized in this study: a random insertional mutagenesis method to discover genes that are required for growth in a low CO2 environment, and a directed mutagenesis approach, RNA interference, to determine …