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Application Of The Biolog Microplate System To Monitor The Physiological Response Of Heat-Stressed Bacteria, Matthew G. Mccarroll
Application Of The Biolog Microplate System To Monitor The Physiological Response Of Heat-Stressed Bacteria, Matthew G. Mccarroll
Graduate Theses, Dissertations, and Problem Reports
The BIOLOG microplate system was originally evaluated by Miller and Rhoden (1991) for its ability to correctly identify clinical and environmental isolates. The system determines identity based on the exchange of electrons produced during utilization of a carbon substrate and subsequent respiration, leading to the reduction of a tetrazolium-based color change. Enteric bacteria ( Escherichia coli, Enterobacter sakazakii, and Salmonella enterica serovar Typhimurium) were exposed to heat stress (52°C) and samples were removed as a function of time (0, 15, 30 and 90 min). Stressed cells were monitored with traditional viable cell counts and inoculated into BIOLOG GN plates. The …
Microbial Ecology Of Freshly Sawn Yellow-Poplar Lumber ( Liriodendron Tulipifera L.) In Two Seasons, Mark Ryan Mikluscak
Microbial Ecology Of Freshly Sawn Yellow-Poplar Lumber ( Liriodendron Tulipifera L.) In Two Seasons, Mark Ryan Mikluscak
Graduate Theses, Dissertations, and Problem Reports
Each year, the forest products industry sustains considerable product loss due to a reduction in market value of lumber brought about by microbial discoloration. The current practice of treating green lumber with chemical biocides to protect it from discoloration, although successful, is a potential hazard to both the environment and human life. One potential alternative to chemical biocides is biological control. The most recent success in the field of biological control has been achieved in the agricultural industry by first studying the microbial ecology of the substrate in which the biologically based preservative is to be used. Literature on microbial …