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Characterization Of Metallothionein Expression In Response To Cadmium In The 267-B1, Normal Prostate Cell Line, Madhuri G. Neriyanuri
Characterization Of Metallothionein Expression In Response To Cadmium In The 267-B1, Normal Prostate Cell Line, Madhuri G. Neriyanuri
Graduate Theses, Dissertations, and Problem Reports
The objective of the study was to characterize the mRNA and protein expression of the MT gene in response to Cd2+ exposure in the 267-B1 immortalized normal human neonatal prostate cell line. The cells were exposed to 18muM Cd2+ for 4hr and rescued for 48hr in P4-8F normal medium. Samples were obtained at 0, 1, 2, 4hr exposure and 4, 8, 12, 16, 24, 36, and 48hr recovery. Basal levels of mRNA for MT-1E, MT-1X, and MT-2A genes in 267-B1 cells were similar to the expression of the G3PDH where as MT-3 had a low basal level expression. Exposure to …
Anatomical And Physiological Properties Of The Superior Paraolivary Nucleus In The Rat, Randy Joseph Kulesza Jr.
Anatomical And Physiological Properties Of The Superior Paraolivary Nucleus In The Rat, Randy Joseph Kulesza Jr.
Graduate Theses, Dissertations, and Problem Reports
The superior paraolivary nucleus (SPON) is a group of neurons located within the superior olivary complex, a constellation of brainstem nuclei involved in auditory processing. The major inputs to the SPON arise from the contralateral ear and SPON axons target primarily the ipsilateral inferior colliculus. However, little is known regarding the neurochemical phenotypes present in the SPON and how these neurons respond to auditory stimuli. Understanding the neurochemical and physiological properties of the constituent neurons will provide insight into the functional role of the SPON and will contribute to our understanding of the neural circuitry involved in hearing. Immunocytochemical, stereological, …
Development Of The Induced Gonadotropin Surge Mechanism In The Prepubertal Heifer, Timothy David Maze
Development Of The Induced Gonadotropin Surge Mechanism In The Prepubertal Heifer, Timothy David Maze
Graduate Theses, Dissertations, and Problem Reports
A longtitudinal study was performed in prepubertal heifer calves to determine when the individual components of the hypothalamic-pituitary-ovarian axis were capable of functioning. In study 1, the hypothalamus and anterior pituitary of prepubertal dairy heifers were challenged with exogenous hormones (estradiol and GnRH, respectively) to determine at what age gonadotropin surges could be induced. Two-, four-, six-, and eight-month-old heifer calves were challenged with GnRH or estradiol. Blood was sampled from the jugular vein every 15 minutes for 6 hours or hourly for 30 hours, for the two treatments, respectively. Heifers in each age group released LH in response to …
Effect Of Peptidoglycan-Polysaccharide Complex On Reproductive Efficiency And Mastitis In Sheep, Ida Holaskova
Effect Of Peptidoglycan-Polysaccharide Complex On Reproductive Efficiency And Mastitis In Sheep, Ida Holaskova
Graduate Theses, Dissertations, and Problem Reports
Bacterial infections associated with mastitis reduce pregnancies in cattle. Effects on pregnancy and incidence of mastitis in sheep were investigated after immunization with peptidoglycan-polysaccharide (PG-PS) and killed cells from Strep. pyogenes. Rambouillet (n = 100) and mixed breed (n = 18) ewe lambs were immunized (∼d42 and 22 before breeding) with PG-PS (30mugPG/kg/bw) or killed cells or were not immunized (Control, n = 117). IgG antibodies were detected by ELISA. Ewes were bred at synchronized estrus. All immunized and half of non-immunized ewes were challenged with PG-PS (60mugPG/kg/bw) d5 after breeding. Although proportion of ewes pregnant at d42 did not …
Effect Of Dietary Protein On Plasma Uric Acid, Body Weight, And Oxidative Stress In Broilers, Maribel Machin
Effect Of Dietary Protein On Plasma Uric Acid, Body Weight, And Oxidative Stress In Broilers, Maribel Machin
Graduate Theses, Dissertations, and Problem Reports
Uric acid is an important antioxidant due to its role as a free radical scavenger, and methods to elevate its plasma concentration may be important in animal health. In a preliminary study, the effect of dietary protein on plasma uric acid (PUA) was determined in 3-wk-old chicks. Twenty-four broiler chicks were randomly assigned to 4 diets: low protein (LP) containing 10% casein, medium protein (MP) containing 20% casein, high protein (HP) containing 45% casein and a commercial diet (COD) for 2-wks. PUA concentration increased in chicks fed HP diet and declined in chicks fed LP. In the principal study, PUA …
Retention Of Early Pregnancy And Its Relationship To Serum Progesterone In Dairy Cattle, Melanie J. Starbuck
Retention Of Early Pregnancy And Its Relationship To Serum Progesterone In Dairy Cattle, Melanie J. Starbuck
Graduate Theses, Dissertations, and Problem Reports
Factors affecting pregnancy maintenance between 28 and 67 days of gestation were studied in dairy cows (N = 211). Beginning on day 28 to 37 post breeding, cows were examined by ultrasonography for a viable pregnancy. Pregnant cows were re-examined for pregnancy losses during the late embryonic (≤day 45) and early fetal stages up to day 67. Overall pregnancy loss was 11%, mostly occurring before day 45. Cows with two corpora lutea maintained fewer pregnancies than cows with one corpus luteum. Pregnancy retention was less when concentrations of progesterone were low during days 28 to 37. The embryo apparently died …