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Investigating Soil Microbiome Changes During Panax Quinquefolius Cultivation In New Gardens, Pok Man Paul Wan
Investigating Soil Microbiome Changes During Panax Quinquefolius Cultivation In New Gardens, Pok Man Paul Wan
Electronic Thesis and Dissertation Repository
Ginseng (Panax quinquefolius) is susceptible to ginseng replant disease (GRD), resulting in root rot and decreased yield when ginseng is grown in soils previously used for ginseng crops. Although fungal and oomycete pathogens are implicated, GRD is a complex disease and the changes in the soil when initially cultivating ginseng that lead to GRD remain unclear. This thesis reported changes in the soil microbiome from three new gardens in Norfolk County, Ontario over three years starting from seeding with ginseng. Over this period of cultivation, metabarcoding of the V4 region of the 16S ribosomal RNA gene clearly showed …
Root-Inhabiting And Rhizosphere Mycobiomes And Crop Yield Of Corn And Wheat, Marianna E. Wallace
Root-Inhabiting And Rhizosphere Mycobiomes And Crop Yield Of Corn And Wheat, Marianna E. Wallace
Electronic Thesis and Dissertation Repository
The root mycobiome plays a direct role in plant productivity, and the study of its community composition allows for identification of organisms that influence plant health. To better understand the role of fungal community composition in crop productivity, the root-associated mycobiomes of historically high and low yield sites of corn and wheat planted in rotation were characterized and analyzed along with soil physicochemical variables and crop yield. In each field studied, root and rhizosphere mycobiomes reflected significant differences in fungal species composition. Several soil variables were found to be predictors of differences in composition of sample types including soil texture …
Manipulating The Root Mycobiome To Improve Plant Performance And Reduce Pathogen Pressure In Corn (Zea Mays), Noor F. Saeed Cheema
Manipulating The Root Mycobiome To Improve Plant Performance And Reduce Pathogen Pressure In Corn (Zea Mays), Noor F. Saeed Cheema
Electronic Thesis and Dissertation Repository
Crop yield often varies within a field of a single genetically uniform crop plant, with the causes presumed to be a mix of both biotic and abiotic factors. Manipulating crop root mycobiomes could potentially increase yield by reducing pathogen impacts and improving access to soil water and nutrients. This study aimed to identify different fungal inoculation treatments that could increase the growth of corn seedlings sown in low productivity soils to that in high productivity soils and shift the root mycobiome composition. Fungal inoculation treatments did not have significantly different root mycobiome composition than seedlings grown in low yield control …
Dynamics Of The Soil Microbiome In Ginseng Gardens, Megan E. Lambert
Dynamics Of The Soil Microbiome In Ginseng Gardens, Megan E. Lambert
Electronic Thesis and Dissertation Repository
Ginseng Replant Disease (GRD) is a syndrome in which ginseng cannot be cultivated in soil previously used to grow ginseng. Since GRD can persist for decades, it severely impacts the Ontario ginseng industry. To better understand the origin of GRD, the impact of ginseng cultivation on the soil microbiome was investigated in bulk soil of three newly planted ginseng gardens in Ontario, from seeding through two years of cultivation. While specific trends in species richness, diversity and composition were unclear, PERMANOVA analyses confirmed that they changed over time. Known GRD-related pathogens, including Ilyonectria mors-panacis and Fusarium oxysporum, were detected in …
Characterizing The Diversity Of The Eukaryotic Microbiome In Marine Crustacean Zooplankton., Rose-Lynne Savage
Characterizing The Diversity Of The Eukaryotic Microbiome In Marine Crustacean Zooplankton., Rose-Lynne Savage
Electronic Thesis and Dissertation Repository
Understanding zooplankton productivity is critical for modeling marine food web function, of which one poorly known factor is the influence of zooplankton symbionts. Zooplankton protist symbiont diversity is underestimated due to the limited surveys and techniques previously used. Using 18S V4 metabarcoding, I characterized the eukaryotic microbiomes associated with crustacean zooplankton from the northern Strait of Georgia, BC. Apostome ciliates were most abundant in all hosts except for cyclopoid copepods, which were dominated by Syndiniales. Most symbiont lineages were more abundant in one or two hosts, suggesting some degree of host preference. Microbiome data also provided information on diet, confirming …