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Evaluation Of Bacteriophage And Viral Persistence During Alkaline Stabilization In Sludge And Biosolids Intended For Land Application, Jacqueline Joy Brabants
Evaluation Of Bacteriophage And Viral Persistence During Alkaline Stabilization In Sludge And Biosolids Intended For Land Application, Jacqueline Joy Brabants
Doctoral Dissertations
The use of lime to reduce or eliminate pathogen content is a cost-effective treatment method currently being employed in many Class B biosolids production plants in the United States. A bench scale model of lime stabilization was designed to evaluate pathogen persistence. The survivability of poliovirus type 1, fecal coliforms, Salmonella, adenovirus type 5, rotavirus Wa, and the male-specific bacteriophage MS-2 was evaluated under lime stabilization conditions in various matrices. Salmonella and fecal coliforms were evaluated at 28°C and poliovirus and MS-2 were evaluated at both 28°C and 4°C for survivability under lime stabilization conditions in a sludge matrix. All …
Assessment Of The Fate Of Cryptosporidium Parvum, Giardia Lamblia And Ascaris Lumbricoides In Class A Composting And Class B Lime Stabilization Biosolids Treatment Processes, Christine L. Bean
Doctoral Dissertations
The management of biosolids in the United States has become an issue of public health concern in regard to disposal. Congress created the Clean Water Act in 1972 to protect waters in the United States and as a result, no longer can human waste be dumped into oceans or rivers. Means of disposal of biosolids are focused on land application today. The practice of land application takes into consideration the classification of the biosolids material and management practices to control access to and growth of crops on the land. Biosolids are classified as either Class A or Class B depending …
Characterization Of Prokaryotic Diversity In A Chlorinated Solvent-Contaminated Bedrock Aquifer Using Denaturing Gradient Gel Electrophoresis, Ribosomal Dna Sequencing, And Real Time Pcr, Walid Naser
Doctoral Dissertations
A culture-independent molecular phylogenetic approach was implemented to investigate spatial and temporal variations in microbial diversity and abundance in a chlorinated solvent-contaminated bedrock aquifer. Core and groundwater samples were collected from several boreholes and included the following samples: (1) groundwater that was associated with the core, (2) open fractures and (3) partially mineralized sealed fractures (PMSF) exposed by striking the core with a surface-sterilized geology hammer. DNA representing the attached and unattached microbial communities was extracted from these samples, and the 16S rRNA gene was amplified with primers specific for Bacteria, Archaea, sulfate reducers, Geobacteraceae family, Dehalorespirers (Dehalococcoides sp. and …