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Development Of A Sandwich Enzyme-Linked Immunosorbent Assay (Elisa) For Detection Of Macadamia Nut Residues In Processed Food Products, Charlene Gan
Department of Food Science and Technology: Dissertations, Theses, and Student Research
Macadamia nut is considered a priority food allergen and undeclared macadamia nut residues pose a potential food safety risk to individuals with macadamia nut allergies. To date, there are a limited number of immunochemical methods that are available for detection of processed macadamia nut residues. Therefore, the aim of this study was to develop a reliable and robust sandwich ELISA for detection of macadamia nut residues. Raw and roasted macadamia nuts were used for immunization in three different species of animals (sheep, goat, and rabbits). Macadamia nut specific IgG antibodies produced by each animal were tested for their specificity and …