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Biochemical Study Of Engineered Fluorescent Proteins As Calcium Sensors And The Effect Of Calcium And Ph In Cell Reproduction And Protein Expression, Malcom Arturo Delgado
Biochemical Study Of Engineered Fluorescent Proteins As Calcium Sensors And The Effect Of Calcium And Ph In Cell Reproduction And Protein Expression, Malcom Arturo Delgado
Chemistry Theses
Calcium plays important roles in both eukaryotic and prokaryotic cells. Its actions help to stabilize cell synthesis, growth and development. In this thesis, studies have been completed to determine effects of calcium and pH on bacterial cell growth and protein expression using the bacterial cell strain E.coli BL21(DE3). Our studies demonstrated the addition of calcium addition in the media does not affect growth but increases protein expression, while reducing the pH from 7 to 4 through the addition of 10mM EGTA in LB media inhibits both. Additionally, we report studies on the design, expression, and purification of fluorescent mCherry variants …