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Treatment Of Pea (Pisum Sativum L.) Protoplasts With Dna-Damaging Agents Induces A 39-Kilodalton Chloroplast Protein Immunologically Related To Escherichia Coli Reca, Heriberto D. Cerutti, H. Z. Ibrahim, A. T. Jagendorf
Treatment Of Pea (Pisum Sativum L.) Protoplasts With Dna-Damaging Agents Induces A 39-Kilodalton Chloroplast Protein Immunologically Related To Escherichia Coli Reca, Heriberto D. Cerutti, H. Z. Ibrahim, A. T. Jagendorf
Center for Plant Science Innovation: Faculty and Staff Publications
Organisms must have efficient mechanisms of DNA repair and recombination to prevent alterations in their genetic information due to DNA damage. There is evidence for DNA repair and recombination in plastids of higher plants, although very little is known at the biochemical level. Many chloroplast proteins are of eubacterial ancestry, suggesting that the same could be true for the components of a DNA repair and recombination system. A 39-kD protein, immunologically related to Escherichia coli RecA, is present in chloroplasts of pea (Pisum sativum L.). Bandshift gel assays suggest that it binds single-stranded DNA. Its steady-state level is increased …
Dna Strand-Transfer Activity In Pea (Pisum Sativum L.) Chloroplasts, Heriberto D. Cerutti, A. T. Jagendorf
Dna Strand-Transfer Activity In Pea (Pisum Sativum L.) Chloroplasts, Heriberto D. Cerutti, A. T. Jagendorf
Center for Plant Science Innovation: Faculty and Staff Publications
The occurrence of DNA recombination in plastids of higher plants is well documented. However, little is known at the enzymic level. To begin dissecting the biochemical mechanism(s) involved we focused on a key step: strand transfer between homologous parental DNAs. We detected a RecA-like strand transfer activity in stromal extracts from pea (Pisum sativum L.) chloroplasts. Formation of joint molecules requires Mg2+, ATP, and homologous substrates. This activity is inhibited by excess single-stranded DNA (ssDNA), suggesting a necessary stoichiometric relation between enzyme and ssDNA. In a novel assay with Triton X-100-permeabilized chloroplasts, we also detected strand invasion of the …