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Fidelity Of Select Restriction Endonucleases In Determining Microbial Diversity By Terminal-Restriction Fragment Length Polymorphism, Craig L. Moyer, Jeff J. Engebretson
Fidelity Of Select Restriction Endonucleases In Determining Microbial Diversity By Terminal-Restriction Fragment Length Polymorphism, Craig L. Moyer, Jeff J. Engebretson
Biology Faculty and Staff Publications
An evaluation of 18 DNA restriction endonucleases for use in terminal-restriction fragment length polymorphism (T-RFLP) analysis was performed by using richness and density indices in conjunction with computer simulations for 4,603 bacterial small-subunit rRNA gene sequences. T-RFLP analysis has become a commonly used method for screening environmental samples for precursory identification and community comparison studies due to its precision and high-throughput capability. The accuracy of T-RFLP analysis for describing a community has not yet been thoroughly evaluated. In this study, we attempted to classify restriction endonucleases based upon the ability to resolve unique terminal-restriction fragments (T-RFs) or operational taxonomic units …