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Tbrap1 Has An Unusual Duplex Dna Binding Activity Required For Its Telomere Localization And Vsg Silencing, Marjia Afrin, Amit Kumar Gaurav, Xian Yang, Xuehua Pan, Yanxiang Zhao, Bibo Li Ph.D.
Tbrap1 Has An Unusual Duplex Dna Binding Activity Required For Its Telomere Localization And Vsg Silencing, Marjia Afrin, Amit Kumar Gaurav, Xian Yang, Xuehua Pan, Yanxiang Zhao, Bibo Li Ph.D.
Biological, Geological, and Environmental Faculty Publications
Localization of Repressor Activator Protein 1 (RAP1) to the telomere is essential for its telomeric functions. RAP1 homologs either directly bind the duplex telomere DNA or interact with telomere-binding proteins. We find that Trypanosoma brucei RAP1 relies on a unique double-stranded DNA (dsDNA) binding activity to achieve this goal. T. brucei causes human sleeping sickness and regularly switches its major surface antigen, variant surface glycoprotein (VSG), to evade the host immune response. VSGs are monoallelically expressed from subtelomeres, and TbRAP1 is essential for VSG regulation. We identify dsDNA and single-stranded DNA binding activities in TbRAP1, which require positively charged 737RKRRR741 …
The More We Do, The Less We Gain? Balancing Effort And Efficacy In Managing The Solidago Gigantea Invasion, Dávid U. Nagy, Emily S.J. Rauschert, Tamás Henn, Kevin Cianfaglione, Szilvia Stranczinger, Robert W. Pal
The More We Do, The Less We Gain? Balancing Effort And Efficacy In Managing The Solidago Gigantea Invasion, Dávid U. Nagy, Emily S.J. Rauschert, Tamás Henn, Kevin Cianfaglione, Szilvia Stranczinger, Robert W. Pal
Biological, Geological, and Environmental Faculty Publications
Developing invasive plant management strategies is an important task in modern ecology, conservation biology and land management. Solidago gigantea is considered a problematic invader in Europe and Asia, where it forms dominant stands that can decrease species diversity. There is, therefore, an urgent need for effective management to reduce S. gigantea infestations and their negative impacts. We examined the efficacy of multiple approaches to S. gigantea management in Hungary. In our study, we evaluated the effect of several long-term management techniques such as grazing, mowing and periodic flooding on the invader's density as well as native community diversity. In addition, …
Basal Signalling Through Death Receptor 5 And Caspase 3 Activates P38 Kinase To Regulate Serum Response Factor (Srf)-Mediated Myod Transcription, Jason A. Ross, Brianna Barrett, Victoria Bensimon, Girish Shukla, Crystal M. Weyman
Basal Signalling Through Death Receptor 5 And Caspase 3 Activates P38 Kinase To Regulate Serum Response Factor (Srf)-Mediated Myod Transcription, Jason A. Ross, Brianna Barrett, Victoria Bensimon, Girish Shukla, Crystal M. Weyman
Biological, Geological, and Environmental Faculty Publications
We have previously reported that stable expression of a dominant negative Death Receptor 5 (dnDR5) in skeletal myoblasts results in decreased basal caspase activity and decreased mRNA and protein expression of the muscle regulatory transcription factor MyoD in growth medium (GM), resulting in inhibited differentation when myoblasts are then cultured in differentiation media (DM). Further, this decreased level of MyoD mRNA was not a consequence of altered message stability, but rather correlated with decreased acetylation of histones in the distal regulatory region (DRR) of the MyoD extended promoter known to control MyoD transcription. As serum response factor (SRF) is the …
Trypanosoma Brucei Rap1 Has Essential Functional Domains That Are Required For Different Protein Interactions, Marjia Afrin, Hanadi Kishmiri, Ranjodh Sandhu, M. A.G. Rabbani, Bibo Li
Trypanosoma Brucei Rap1 Has Essential Functional Domains That Are Required For Different Protein Interactions, Marjia Afrin, Hanadi Kishmiri, Ranjodh Sandhu, M. A.G. Rabbani, Bibo Li
Biological, Geological, and Environmental Faculty Publications
RAP1 is a telomere protein that is well conserved from protozoa to mammals. It plays important roles in chromosome end protection, telomere length control, and gene expression/silencing at both telomeric and nontelomeric loci. Interaction with different partners is an important mechanism by which RAP1 executes its different functions in yeast. The RAP1 ortholog in Trypanosoma brucei is essential for variant surface glycoprotein (VSG) monoallelic expression, an important aspect of antigenic variation, where T. brucei regularly switches its major surface antigen, VSG, to evade the host immune response. Like other RAP1 orthologs, T. brucei RAP1 (TbRAP1) has conserved functional domains, including …