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Industrial Grade 2d Molybdenum Disulphide (Mos2): An In-Vitro Exploration Of The Impact On Cellular Uptake, Cytotoxicity, And Inflammation, Caroline Moore, Hugh Byrne, Jonathan N. Coleman, Yuri Volkov, Jennifer Mcintyre
Industrial Grade 2d Molybdenum Disulphide (Mos2): An In-Vitro Exploration Of The Impact On Cellular Uptake, Cytotoxicity, And Inflammation, Caroline Moore, Hugh Byrne, Jonathan N. Coleman, Yuri Volkov, Jennifer Mcintyre
Articles
The recent surge in graphene research, since its liquid phase monolayer isolation and characterization in 2004, has led to advancements which are accelerating the exploration of alternative 2D materials such as molybdenum disulphide (MoS2), whose unique physico-chemical properties can be exploited in applications ranging from cutting edge electronic devices to nanomedicine. However, to assess any potential impact on human health and the environment, the need to understand the bio-interaction of MoS2 at a cellular and sub-cellular level is critical. Notably, it is important to assess such potential impacts of materials which are produced by large scale production techniques, rather than …
Monitoring Doxorubicin Cellular Uptake And Trafficking Using In Vitro Raman Microspectroscopy: Short And Long Time Exposure Effects On Lung Cancer Cell Lines, Zeineb Farhane, Franck Bonnier, Hugh Byrne
Monitoring Doxorubicin Cellular Uptake And Trafficking Using In Vitro Raman Microspectroscopy: Short And Long Time Exposure Effects On Lung Cancer Cell Lines, Zeineb Farhane, Franck Bonnier, Hugh Byrne
Articles
Raman microspectroscopy is a non-invasive, in vitro analytical tool which is being increasingly explored for its potential in clinical applications and monitoring the uptake, mechanism of action and cellular interaction at a molecular level of chemotherapeutic drugs, ultimately as a potential label-free preclinical screening and companion diagnostic tool. In this study, doxorubicin (DOX), a “gold standard” chemotherapeutic drug, is employed as a model in the in vitro lung cancer cell line A549 in order to demonstrate the potential of Raman microspectroscopy to screen and identify spectroscopic markers of its trafficking and mechanism of action. Confocal laser scanning microscopy (CLSM) was …
Highly Branched Poly(5-Amino-1-Pentanol-Co-1,4- Butanediol Diacrylate) For High Performance Gene Transfection, Ming Zeng, Dezhong Zhou, Singwei Ng, Jonathan O'Keefe Ahern, Fatma Alshehri, Yongsheng Gao, Luca Pierucci, Udo Greiser, Wenxin Wang
Highly Branched Poly(5-Amino-1-Pentanol-Co-1,4- Butanediol Diacrylate) For High Performance Gene Transfection, Ming Zeng, Dezhong Zhou, Singwei Ng, Jonathan O'Keefe Ahern, Fatma Alshehri, Yongsheng Gao, Luca Pierucci, Udo Greiser, Wenxin Wang
Articles
The top-performing linear poly(β-amino ester) (LPAE), poly(5-amino-1-pentanol-co-1,4-butanediol diacrylate) (C32), has demonstrated gene transfection efficiency comparable to viral-mediated gene delivery. Herein, we report the synthesis of a series of highly branched poly(5-amino-1-pentanol-co-1,4-butanediol diacrylate) (HC32) and explore how the branching structure influences the performance of C32 in gene transfection. HC32 were synthesized by an “A2 + B3 + C2” Michal addition strategy. Gaussia luciferase (Gluciferase) and green fluorescent protein (GFP) coding plasmid DNA were used as reporter genes and the gene transfection efficiency was evaluated in human cervical cancer cell line (HeLa) and human recessive dystrophic epidermolysis bullosa keratinocyte (RDEBK) cells. We …
Determination Of Spectral Markers Of Cytotoxicity And Genotoxicity Using In Vitro Raman Microspectroscopy: Cellular Responses To Polyamidoamine Dendrimer Exposure, Esen Efeoglu, Alan Casey, Hugh Byrne
Determination Of Spectral Markers Of Cytotoxicity And Genotoxicity Using In Vitro Raman Microspectroscopy: Cellular Responses To Polyamidoamine Dendrimer Exposure, Esen Efeoglu, Alan Casey, Hugh Byrne
Articles
Although consumer exposure to nanomaterials is ever increasing, with potential increased applications in areas such as drug and/or gene delivery, contrast agents and diagnosis, determination of cyto- and geno- toxic effect of nanomaterials on human health and the environment still remains challenging. Although many techniques have been established and adapted to determine the cytotoxicity and genotoxicity of nano-sized materials, these techniques remain limited by the number of assays required, total cost, use of labels and they struggle to explain the underlying interaction mechanisms. In this study, Raman microspectroscopy is employed as an in vitro label free high content screening technique …
Highly Branched Poly(5-Amino-1-Pentanol-Co-1,4-Butanediol Diacrylate) For High Performance Gene Transfection, Ming Zheng, Dezhong Zhou, Singwei Ng, Jonathan O'Keefe Ahern, Fatma Alshehri, Yongsheng Gao, Luca Pierucci, Udo Greiser, Wenxin Wang
Highly Branched Poly(5-Amino-1-Pentanol-Co-1,4-Butanediol Diacrylate) For High Performance Gene Transfection, Ming Zheng, Dezhong Zhou, Singwei Ng, Jonathan O'Keefe Ahern, Fatma Alshehri, Yongsheng Gao, Luca Pierucci, Udo Greiser, Wenxin Wang
Articles
The top-performing linear poly(β-amino ester) (LPAE), poly(5-amino-1-pentanol-co-1,4-butanediol diacrylate) (C32), has demonstrated gene transfection efficiency comparable to viral-mediated gene delivery. Herein, we report the synthesis of a series of highly branched poly(5-amino-1-pentanol-co-1,4-butanediol diacrylate) (HC32) and explore how the branching structure influences the performance of C32 in gene transfection. HC32 were synthesized by an “A2 + B3 + C2” Michal addition strategy. Gaussia luciferase (Gluciferase) and green fluorescent protein (GFP) coding plasmid DNA were used as reporter genes and the gene transfection efficiency was evaluated in human cervical cancer cell line (HeLa) and human recessive dystrophic epidermolysis bullosa keratinocyte (RDEBK) cells. We …