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Supersize It! Developing A "Supercytoplast" Through Platelet Fusion, Taulbee B. Gunter

Animal Science Undergraduate Honors Theses

Stem cell research opens a wide range of possibilities from lab-grown tissues for medical purposes to cloned animals for livestock production. The issue with one of the most popular forms of cell recombination into a stem cell, known as somatic cell nuclear transfer (SCNT), is that it requires an oocyte. The requirement of an oocyte is often the host of public scrutiny for its economic and ethical issues. Platelets may offer a less expensive and more accessible alternative to oocytes as sources of naturally enucleated cells. Thus, the objective of this explorative research project is to isolate and fuse platelets …

Pilot Testing A Qualitative Methodology To Evaluate The Perceived Benefits And Stressors Of Sole-Dog Ownership In Full-Time College Students, Emma Williams

Animal Science Undergraduate Honors Theses

The number of individuals acquiring pets is continually increasing, with 71.5% of Americans owning a pet in 2012. Companion animals are a huge source of benefits but owning them can also come with several stressors. However, little research has been conducted on the negative aspects of dog ownership. This pilot test evaluates the perceived stressors and benefits of sole dog ownership in full-time college students. The pilot aimed to hold three focus groups from which qualitative thematic data were collected and quantitative were collected from a demographic survey of participants. Participants were asked a series of questions about sole dog …

The Effects Of Storage Temperature On The Bovine Nasal Microbiome, Hunter Usdrowski

Animal Science Undergraduate Honors Theses

Bovine respiratory disease (BRD) is one of the largest economic burdens facing United States beef producer’s modern day. The complexity in the onset and development of this multifactorial disease necessitates further insights into its functions to alleviate the tremendous consequences it generates for producers. The respiratory microbiome and their metabolomics play an important role to maintain health and predict BRD. However, due to low biomass, new technology is needed to increase the microbial concentration for omics (e.g. metatranscriptomics) research. In this study, a novel self-enrichment storage technique on the bovine nasal microbiome was performed and compared to a traditional nasal …

Mechanistic Understanding Of Leaky Gut Syndrome In Heat Stressed Broiler Chickens, Jennifer Hoover

Animal Science Undergraduate Honors Theses

Abstract

Broilers, meat-type chickens, are bred for high growth rate and meat yield. These chickens have specific challenges due to heat stress. The adverse effects that impact these chickens have lead us to study the mechanisms involved. The objective of this study is to evaluate the expression of genes involved in intestinal permeability of heat- stressed broiler chickens. All analyses are on previously collected tissues. A total of 600 one day old Cobb 500 male chicks were weighed and randomly assigned to 12 environmental chambers. Ambient temperature in the chambers was gradually decreased from 32˚C on d 1 to 24˚C …

Effect Of Zelnate Administered As A Metaphylactic Upon Initial Processing Of High-Risk, Newly Received Beef Calves On Performance And Morbidity, Brady Martin

Animal Science Undergraduate Honors Theses

Bovine Respiratory Disease (BRD) is the most prominent and costly ailment in the stocker cattle industry today, and its prevalence has not been diminished in the last thirty years. Therefore, the objective of this study was to investigate the effects of Zelnate, a DNA immunostimulant, administered upon arrival to calves (n = 261; BW 253 ± 4.0 kg), on morbidity/mortality, performance and producer costs. Crossbred male beef calves were acquired and transported to the University of Arkansas stocker unit for a 42-d backgrounding period. Calves were allocated into treatment groups: 1) Zelnate, DNA immunostimulant administered or 2) Control, in which …

Use Of Diatomaceous Earth And Copper Oxide Wire Particles To Control Gastrointestinal Nematodes In Lambs, Olivia Jones

Animal Science Undergraduate Honors Theses

Abstract

Anthelmintic resistance (AR) urges alternatives to control gastrointestinal nematodes (GIN). Copper oxide wire particles (COWP) are more efficacious when used with other dewormers and little is known on efficacy of diatomaceous earth (DE) to control gastrointestinal parasites. The objective was to examine the effects of DE and COWP on GIN control. Katahdin lambs (n = 32; ~150 d of age; 25.0 ± 1.8 kg) were randomly assigned to receive: 1) DE fed at an estimated 2% dry matter intake (with the assumption of moderate consumption of bermudagrass forage and provided supplement), 2) 1g COWP, 3) both 2% DE and …

Impact Of Phosphorus Intake On Beef Heifer Growth Performance And Conception Rates, Hailey Hilfiker

Animal Science Undergraduate Honors Theses

This study aims to examine the effects of phosphorus intake on beef heifer growth performance and conception rates. In Northwest Arkansas, there has been an increase in phosphorus concentrations in soil where livestock manures have been repeatedly applied. Consequently, forages grown on soils high in phosphorus tend to contain high amounts of the mineral itself. This has led many to question whether it is necessary to supplement phosphorus in areas where concentrations may be higher. In this study, crossbred Angus heifers (n = 72), approximately 30 days after weaning, were stratified by body weight (average initial weight 251 ± 3.9 …

Association Of Single Nucleotide Polymorphisms In Bovine Heat Shock Protein 70, Cytochrome P450, Lactate Dehydrogenase, Interleukin 8 Receptor, And Melatonin Receptor 1a With Hoof And Udder Traits In Beef Cows, Maryrose Warren

Animal Science Undergraduate Honors Theses

Hoof and udder conformation are important to the longevity and productivity of beef cattle. Selection for hoof and udder quality reduces the incidence of lameness and udder disease to improve animal welfare. Genetic selection is an effective means of improving traits such as these, especially through the use of single nucleotide polymorphism (SNP) genotyping to identify indicator traits. This study used genotype data and hoof and udder scores from previous research to determine if there is significant effect between polymorphisms of heat shock protein 70 (Hsp70), cytochrome P450 (CYP450), interleukin-8 receptor (IL8), lactate dehydrogenase (LDH), and melatonin receptor 1A (MTNR1A), …

Surveillance Of Anaplasma Marginale In Arkansas Beef Cattle Herds, Gabriel Apple

Animal Science Undergraduate Honors Theses

Anaplasmosis is an economically devastating disease in cattle that is caused by the rickettsial pathogen Anaplasma marginale. It is estimated that this parasitic bacterium causes over $300 million in expenses for the U.S. cattle industry annually. In Arkansas, the beef cattle industry is the fifth largest agricultural commodity in the state, thus necessitating a better understanding of this disease along with its prevalence. In this study, both polymerase chain reaction (PCR) and competitive enzyme-linked immunosorbent assay (ELISA) tests were used to determine the prevalence of A. marginale infection in Arkansas beef cattle on pasture in the six commonly known geographical …

Effect Of Dietary Spirulina Platensis On Stress Levels And Growth Of Female Broiler Chickens, Victoria Tarkington

Animal Science Undergraduate Honors Theses

This study measured the effects of dietary Spirulina platensis supplementation on heterophil to lymphocyte ratio, footpad lesions, and growth performance parameters. One hundred and eighty Ross 708 broiler females were randomly divided evenly into fifteen pens with three dietary treatments for a total of five replications per treatment (twelve birds per replicate) for five weeks. The different treatments were a control diet of 20% crude protein, a diet of 17% crude protein, and a diet of 17% crude protein with 10% S. platensis added to the feed. Body weight, feed intake, and mortality rate was not significantly different between treatments …

The Efficacy Of Extended-Release Eprinomectin For The Reduction Of Horn Flies, Face Flies, And Fecal Egg Counts Of Parasitic Nematodes In Replacement Beef Heifers, Sophia F. Landers

Animal Science Undergraduate Honors Theses

The purpose of this study was to evaluate the efficacy of extended-release eprinomectin against horn flies, face flies, and fecal egg counts of parasitic nematodes in crossbreed replacement beef heifers. Fifty-four heifers were randomly placed into three treatment groups (N=18 heifers/treatment). Group 1 was administered the labeled dosage of extended-release eprinomectin on day 0. Group 2 acted as the negative control. Group 3 received the anthelmintic injection once a quarter of the heifers in the group reached the threshold treatment level for horn flies (N=200 flies/animal; day 41). Nematode infections were measured via fecal egg counts while horn and face …