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Dynamic Characterization Of The Ikk:Κbα:Nfκb Negative Feedback Loop Using Real-Time Bioluminescence Imaging, Britney Moss
Dynamic Characterization Of The Ikk:Κbα:Nfκb Negative Feedback Loop Using Real-Time Bioluminescence Imaging, Britney Moss
All Theses and Dissertations (ETDs)
The transcription factor NF-κB is a pivotal regulator of mammalian cell function, modulating genes implicated in cellular stress responses, proliferation, differentiation, cell survival and apoptosis, as well as immune and inflammatory responses. Improper regulation of NF-κB signaling has been implicated in a myriad of human pathological disorders, including cardiovascular and neurodegenerative diseases, chronic inflammation, and various cancers. A key regulatory node within canonical NF-κB signaling is the IKK:NF-κB: IκBα negative feedback loop that plays a major role in regulating the strength and duration of NF-κB transcriptional activity. We have developed and characterized an unique bioluminescent reporter (κB5àIκBα-FLuc) …
Specific Roles Of Macaque Parietal Regions In Making Saccades And Reaches, Eric Yttri
Specific Roles Of Macaque Parietal Regions In Making Saccades And Reaches, Eric Yttri
All Theses and Dissertations (ETDs)
A principle task of our brain is to guide movements, includng saccade: fast eye movements) and reaches towards things that we see. Regions in the parietal cortex such as LIP and PRR are active during visually-guided movements. Neurons in these areas respond differentially for saccades versus reaches, but in most parietal areas there is some response: in single unit recording as well as in fMRI imaging) with either type of movement. This raises an important question. What is the functional significance of the neuronal activity in parietal areas? Recording and imaging studies can only show correlations; causal roles must be …
Studies Of Fluorescent Imaging For Mrna Detection In Living Cells, Zhenghui Wang
Studies Of Fluorescent Imaging For Mrna Detection In Living Cells, Zhenghui Wang
All Theses and Dissertations (ETDs)
This dissertation focuses on the study of imaging mRNA in living cells. To achieve this research objective, three approaches have been utilized:: 1) Imaging of a transgenic mRNA tagged by multiple repeats of malachite green: MG) binding aptamer.: 2) Imaging of inducible nitric oxide synthase: iNOS) mRNA by strand-displacement activated Peptide Nucleic Acid: PNA) probes.: 3) Imaging of iNOS mRNA by binary fluorescently labeled PNA probes. The first approach was based on the work of our former lab member Dr. Huafeng Fang, who had constructed a multiple MG binding aptamer tagged transgene: Flag-mβ2AR-GFP-MGVI), which could also express a green fluorescence …