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Investigating The Role Of Adp-Forming Acetyl-Coa Synthetase From The Protozoan Parasite Entamoeba Histolytica, Cheryl Page Jones

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ADP-forming acetyl-CoA synthetase (ACD; EC 6.2.1.13) catalyzes the reversible conversion of acetyl-CoA to acetate coupled to the production of ATP. This enzyme is present only in certain acetate-producing archaea and a limited number of bacteria and eukaryotes. ACD belongs to the same NDP-forming acyl-CoA synthetase enzyme superfamily as succinyl-CoA synthetase (SCS; EC 6.2.1.4) from the citric acid cycle, and a similar three-step mechanism involving a phosphoenzyme intermediate was originally proposed for this enzyme. ACD has been postulated to be a major acetate-producing enzyme in the protozoan parasite Entamoeba histolytica and may contribute to ATP production. Biochemical and kinetic characterization of …