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Using Confocal Microscopy In The Study Of Plant Structure And Development, Mark P. Running, Elliot M. Meyerowitz
Using Confocal Microscopy In The Study Of Plant Structure And Development, Mark P. Running, Elliot M. Meyerowitz
Aliso: A Journal of Systematic and Floristic Botany
The widespread application of confocal laser scanning microscopy has revolutionized biological imaging. We have developed a protocol for using confocal microscopy to examine the development of wild type and mutant Arabidopsis thaliana, overcoming the technical difficulties associated with examining whole-mounted plant tissue. This allowed us to rapidly determine the underlying cellular defects that lead to the morphological changes visible in several mutants, and has led to a greater understanding of the mechanisms involved in the control of floral organ number.