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- Antiviral (3)
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- Anisandrus maiche; Xylosandrus germanus; Scolytinae; long-lasting insecticide netting; deltamethrin (1)
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Articles 1 - 30 of 43
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Urban Horticulture, From Local Initiatives To Global Success Stories, Roland Ebel, Esmaeil Fallahi, John L. Griffis Jr., Dilip Nandwani, Donielle Nolan, Ross H. Penhallegon, Mary Rogers
Urban Horticulture, From Local Initiatives To Global Success Stories, Roland Ebel, Esmaeil Fallahi, John L. Griffis Jr., Dilip Nandwani, Donielle Nolan, Ross H. Penhallegon, Mary Rogers
Agricultural and Environmental Sciences Faculty Research
Urban horticulture describes economically viable horticultural production activities conducted in a city or suburb. It is a growing segment of horticulture in the United States as well as in developing countries, where the enormous growth of megalopolis is not backed by a simultaneous increase of farmland or agricultural productivity. Today, urban horticulture includes food sovereignty in underprivileged neighborhoods, increased availability of vegetables and fruits in big cities, healthy and diverse diets, improved food safety, low transportation costs, efficient resource use, and the mitigation of environmental impacts of horticultural production such as the emission of greenhouse gases. The workshop “Urban horticulture: …
Xenopus Interferon Complex: Inscribing The Amphibiotic Adaption And Species-Specific Pathogenic Pressure In Vertebrate Evolution?, Yun Tian, Jordan Jennings, Yuanying Gong, Yongming Sang
Xenopus Interferon Complex: Inscribing The Amphibiotic Adaption And Species-Specific Pathogenic Pressure In Vertebrate Evolution?, Yun Tian, Jordan Jennings, Yuanying Gong, Yongming Sang
Agricultural and Environmental Sciences Faculty Research
Several recent studies have revealed previously unknown complexity of the amphibian interferon (IFN) system. Being unique in vertebrate animals, amphibians not only conserve and multiply the fish-like intron-containing IFN genes, but also rapidly evolve amniote-like intronless IFN genes in each tested species. We postulate that the amphibian IFN system confers an essential model to study vertebrate immune evolution in molecular and functional diversity to cope with unprecedented pathophysiological requirement during terrestrial adaption. Studies so far have ascribed a potential role of these IFNs in immune regulation against intracellular pathogens, particularly viruses; however, many knowledge gaps remain elusive. Based on recent …
Long-Lasting Insecticide Netting For Protecting Tree Stems From Attack By Ambrosia Beetles (Coleoptera: Curculionidae: Scolytinae), Christopher M. Ranger, Christopher T. Werle, Peter B. Schultz, Karla M. Addesso, Jason B. Oliver, Michael E. Reding
Long-Lasting Insecticide Netting For Protecting Tree Stems From Attack By Ambrosia Beetles (Coleoptera: Curculionidae: Scolytinae), Christopher M. Ranger, Christopher T. Werle, Peter B. Schultz, Karla M. Addesso, Jason B. Oliver, Michael E. Reding
Agricultural and Environmental Sciences Faculty Research
Ambrosia beetles (Coleoptera: Curculionidae: Scolytinae) are destructive wood-boring insects of horticultural trees. We evaluated long-lasting insecticide netting for protecting stems against ambrosia beetles. Container-grown eastern redbud, Cercis canadensis, trees were flood-stressed to induce ambrosia beetle attacks, and deltamethrin-treated netting was wrapped from the base of the stem vertically to the branch junction. Trees were deployed under field conditions in Ohio, Virginia, Tennessee, and Mississippi with the following treatments: (1) flooded tree; (2) flooded tree with untreated netting; (3) flooded tree with treated ‘standard mesh’ netting of 24 holes/cm2; (4) flooded tree with treated ‘fine mesh’ netting of 28 …
Embryogenic Cell Suspensions For High-Capacity Genetic Transformation And Regeneration Of Switchgrass (Panicum Virgatum L.), Christine A. Ondzighi-Assoume, Jonathan D. Willis, Wilson Kihugu Ouma, Sara M. Allen, Zachary King, Wayne A. Parrott, Wusheng Liu, Jason N. Burris, Scott C. Lenaghan, C. Neal Stewart
Embryogenic Cell Suspensions For High-Capacity Genetic Transformation And Regeneration Of Switchgrass (Panicum Virgatum L.), Christine A. Ondzighi-Assoume, Jonathan D. Willis, Wilson Kihugu Ouma, Sara M. Allen, Zachary King, Wayne A. Parrott, Wusheng Liu, Jason N. Burris, Scott C. Lenaghan, C. Neal Stewart
Agricultural and Environmental Sciences Faculty Research
Background
Switchgrass (Panicum virgatum L.), a North American prairie grassland species, is a potential lignocellulosic biofuel feedstock owing to its wide adaptability and biomass production. Production and genetic manipulation of switchgrass should be useful to improve its biomass composition and production for bioenergy applications. The goal of this project was to develop a high-throughput stable switchgrass transformation method using Agrobacterium tumefaciens with subsequent plant regeneration.
Results
Regenerable embryogenic cell suspension cultures were established from friable type II callus-derived inflorescences using two genotypes selected from the synthetic switchgrass variety ‘Performer’ tissue culture lines 32 and 605. The cell suspension cultures …
Expression Of Lysine-Mediated Neuropeptide Hormones Controlling Satiety And Appetite In Broiler Chickens, Collins N. Khwatenge, Boniface M. Kimathi, Thyneice Taylor-Bowden, Samuel N. Nahashon
Expression Of Lysine-Mediated Neuropeptide Hormones Controlling Satiety And Appetite In Broiler Chickens, Collins N. Khwatenge, Boniface M. Kimathi, Thyneice Taylor-Bowden, Samuel N. Nahashon
Agricultural and Environmental Sciences Faculty Research
Lysine is the second most limiting amino acid after methionine and is considered the most limiting amino acid for growth in poultry. Lysine requirement for broiler chickens has changed over the years. Leptin and adiponectin represent 2 adipokines that mediate metabolism by eliciting satiety effects whereas ghrelin peptide hormone influences appetite. We hypothesize that this affects growth performance of chicks. This study evaluates the effect of varying dietary lysine homeostasis on performance of broiler chickens through satiety- and appetite-mediating hormones. In 3 replications, 270 one-day-old chicks were reared for 8 wk feeding on diets comprising 0.85, 1.14, and 1.42% lysine …
Laying Performance Of Pearl Gray Guinea Fowl Hens As Affected By Caging Density, Samuel N. Nahashon, Nathaniel Adefope, Abravi Amenyenu, D. Wright
Laying Performance Of Pearl Gray Guinea Fowl Hens As Affected By Caging Density, Samuel N. Nahashon, Nathaniel Adefope, Abravi Amenyenu, D. Wright
Agricultural and Environmental Sciences Faculty Research
The caging density required for optimal egg production by various avian species and varieties is highly variable. Even so, little is known of the required cage density for optimum performance of the laying guinea fowl (Numida meleagris). The objective of this study was to assess the effect of varying cage densities on production performance of pearl gray guinea fowl laying hens. In 3 replicates, 270 pearl gray guinea hens [28 wk of age (WOA)] were weighed individually and randomly assigned to laying cages at densities of 1, 2, and 3 birds/cage, equivalent to 1,394, 697, and 465 cm2/bird, respectively. During …
Effect Of Varying Dietary Concentrations Of Lysine On Growth Performance Of The Pearl Grey Guinea Fowl, Sarayu Bhogoju, Samuel N. Nahashon, Joseph Donkor, Boniface M. Kimathi, Dekarra Johnson, Collins N. Khwatenge, Thyneice Taylor-Bowden
Effect Of Varying Dietary Concentrations Of Lysine On Growth Performance Of The Pearl Grey Guinea Fowl, Sarayu Bhogoju, Samuel N. Nahashon, Joseph Donkor, Boniface M. Kimathi, Dekarra Johnson, Collins N. Khwatenge, Thyneice Taylor-Bowden
Agricultural and Environmental Sciences Faculty Research
Lysine is the second limiting essential amino acid in poultry nutrition after methionine. Understanding the lysine requirement of poultry is necessary in guiding formulation of least cost diets that effectively meet the nutritional needs of individual birds. The lysine requirement of the Pearl Grey guinea fowl (PGGF) is not known. Therefore, the objective of this study was to assess the appropriate lysine levels required for optimal growth attributes of the PGGF. In a 12-week study, 512 one-day-old Pearl Grey guinea keets were weighed individually and randomly assigned to electrically heated battery brooders. Each battery contained 12 compartments housing 15 birds …
Modeling Growth Characteristics Of Meat-Type Guinea Fowl, Samuel N. Nahashon, Sammy E. Aggrey, Nathaniel Adefope, Abravi Amenyenu
Modeling Growth Characteristics Of Meat-Type Guinea Fowl, Samuel N. Nahashon, Sammy E. Aggrey, Nathaniel Adefope, Abravi Amenyenu
Agricultural and Environmental Sciences Faculty Research
This study was conducted to describe the growth pattern of the French guinea fowl, a meat-type variety. Using BW data from hatch to 9 wk, 2 nonlinear mathematical functions (Gompertz and logistic) were used to estimate growth patterns of the French guinea fowl. The French guinea fowl did not exhibit sexual dimorphism for growth characteristics. From the Gompertz model, the asymptotic BW, growth rate, and age at maximum growth were 2.05 kg, 0.25 kg/wk, and 5.74 wk in males, respectively, and 2.03 kg, 0.25 kg/wk, and 5.72 wk in females, respectively. The ages at maximum growth were 5.75 and 5.74 …
Effects Of Dietary Metabolizable Energy And Crude Protein Concentrations On Growth Performance And Carcass Characteristics Of French Guinea Broilers, Samuel N. Nahashon, Nathaniel Adefope, Abravi Amenyenu, D. Wright
Effects Of Dietary Metabolizable Energy And Crude Protein Concentrations On Growth Performance And Carcass Characteristics Of French Guinea Broilers, Samuel N. Nahashon, Nathaniel Adefope, Abravi Amenyenu, D. Wright
Agricultural and Environmental Sciences Faculty Research
This study was undertaken to assess dietary CP and ME concentrations for optimum growth performance and carcass characteristics of French guinea broilers. In a 3 x 3 factorial arrangement, 396 1-d-old French guinea keets were randomly assigned to experimental diets with 3,050, 3,100, and 3,150 kcal of ME/kg of diet; each contained 21, 23, and 25% CP, respectively, from 0 to 4 wk of age (WOA). From 5 to 8 WOA, experimental diets had 3,100, 3,150, and 3,200 kcal of ME/kg of diet, and each contained 19, 21, and 23% CP, respectively. Each dietary treatment was replicated 3 times, and …
Growth Characteristics Of Pearl Gray Guinea Fowl As Predicted By The Richards, Gompertz, And Logistic Models, Samuel N. Nahashon, Sammy E. Aggrey, Nathaniel Adefope, Abravi Amenyenu, D. Wright
Growth Characteristics Of Pearl Gray Guinea Fowl As Predicted By The Richards, Gompertz, And Logistic Models, Samuel N. Nahashon, Sammy E. Aggrey, Nathaniel Adefope, Abravi Amenyenu, D. Wright
Agricultural and Environmental Sciences Faculty Research
This study was undertaken to describe the growth pattern of the pearl gray Guinea fowl. Using BW data from hatch to 22 wk, 3 nonlinear mathematical functions (Richards, Gompertz, and logistic) were used to estimate growth patterns of the pearl gray guinea fowl. The logistic and Gompertz models are a special case of the Richards model, which has a variable point of inflection defined by the shape or growth trajectory parameter, m. The shape parameter m was 1.08 and 0.98 in males and females, respectively, suggesting that the growth pattern of the pearl gray female guinea fowl is Gompertz. The …
Ppnac187 Enhances Lignin Synthesis In ‘Whangkeumbae’ Pear (Pyrus Pyrifolia) ‘Hard-End’ Fruit, Mingtong Li, Chenxia Cheng, Xinfu Zhang, Suping Zhou, Caihong Wang, Chunhui Ma, Shaolan Yang
Ppnac187 Enhances Lignin Synthesis In ‘Whangkeumbae’ Pear (Pyrus Pyrifolia) ‘Hard-End’ Fruit, Mingtong Li, Chenxia Cheng, Xinfu Zhang, Suping Zhou, Caihong Wang, Chunhui Ma, Shaolan Yang
Agricultural and Environmental Sciences Faculty Research
A disorder in pears that is known as ‘hard-end’ fruit affects the appearance, edible quality, and market value of pear fruit. RNA-Seq was carried out on the calyx end of ‘Whangkeumbae’ pear fruit with and without the hard-end symptom to explore the mechanism underlying the formation of hard-end. The results indicated that the genes in the phenylpropanoid pathway affecting lignification were up-regulated in hard-end fruit. An analysis of differentially expressed genes (DEGs) identified three NAC transcription factors, and RT-qPCR analysis of PpNAC138, PpNAC186, and PpNAC187 confirmed that PpNAC187 gene expression was correlated with the hard-end disorder in pear fruit. A …
Household’S Perception And Their Willingness To Pay For Pesticides-Free Fruits In Khyber Pakhtunkhwa (Kp) Province Of Pakistan: A Double-Bounded Dichotomous Choice Contingent Valuation Study, Jahangir Khan, Abbasullah Jan, Kar Ho Lim, Syed Attaullah Shah, Aditya R. Khanal, Ghaffar Ali
Household’S Perception And Their Willingness To Pay For Pesticides-Free Fruits In Khyber Pakhtunkhwa (Kp) Province Of Pakistan: A Double-Bounded Dichotomous Choice Contingent Valuation Study, Jahangir Khan, Abbasullah Jan, Kar Ho Lim, Syed Attaullah Shah, Aditya R. Khanal, Ghaffar Ali
Agricultural and Environmental Sciences Faculty Research
This study investigated household’s willingness to pay (WTP) for pesticide-free locally produced fresh fruits in Khyber Pakhtunkhwa, Pakistan. A Contingent Valuation Survey Method (CVM), constructed with a double-bounded WTP elicitation format, was used to interview a randomly selected sample of 600 households. Survey data revealed that households were willing to pay on average rupees 26 more per kilogram of pesticides free fruits. Results from regression analysis showed that age of the household head and his awareness of pesticides use over fruits, household size and their total monthly income were significant determinants of their WTP. In addition, health concern and environment …
Impact Of Cover Crop Usage On Soilborne Diseases In Field Nursery Production, Sujan Dawadi, Fulya Baysal-Gurel, Karla M. Addesso, Jason B. Oliver, Terri Simmons
Impact Of Cover Crop Usage On Soilborne Diseases In Field Nursery Production, Sujan Dawadi, Fulya Baysal-Gurel, Karla M. Addesso, Jason B. Oliver, Terri Simmons
Agricultural and Environmental Sciences Faculty Research
Soilborne pathogens are a significant economic problem for nursery production in the Southeastern United States. The goal of this study was to determine the impact of cover crops on soilborne disease suppressiveness in such systems. Soils from red maple (Acer rubrum L.) plantation fields grown with and without cover crops were sampled, either while the cover crops were growing (pre-disked) or post-season, following cover crop incorporation into the soil (post-disked). Greenhouse bioassays were conducted using red maple seeds on inoculated (with Rhizoctonia solani (J.G. Kühn) or Phytophthora nicotianae (Breda de Haan)) and non-inoculated field soils. The damping-off, root rot disease …
Viral Infections And Interferons In The Development Of Obesity, Yun Tian, Jordan Jennings, Yuanying Gong, Yongming Sang
Viral Infections And Interferons In The Development Of Obesity, Yun Tian, Jordan Jennings, Yuanying Gong, Yongming Sang
Agricultural and Environmental Sciences Faculty Research
Obesity is now a prevalent disease worldwide and has a multi-factorial etiology. Several viruses or virus-like agents including members of adenoviridae, herpesviridae, slow virus (prion), and hepatitides, have been associated with obesity; meanwhile obese patients are shown to be more susceptible to viral infections such as during influenza and dengue epidemics. We examined the co-factorial role of viral infections, particularly of the persistent cases, in synergy with high-fat diet in induction of obesity. Antiviral interferons (IFNs), as key immune regulators against viral infections and in autoimmunity, emerge to be a pivotal player in the regulation of adipogenesis. In this review, …
Effect Of Winter Canola Cultivar On Seed Yield, Oil, And Protein Content, Edmund T. Tetteh, Jason P. De Koff, Bharat Pokharel, Richard Link, Chris Robbins
Effect Of Winter Canola Cultivar On Seed Yield, Oil, And Protein Content, Edmund T. Tetteh, Jason P. De Koff, Bharat Pokharel, Richard Link, Chris Robbins
Agricultural and Environmental Sciences Faculty Research
Canola (Brassica napus L.) is an oilseed crop that can produce healthy cooking oil and animal feed byproducts. Although it is a relatively new crop, approved for human consumption less than 40 yr ago, advances in breeding have allowed for its production as a winter crop in the southeastern United States. There is little published research, however, related to its performance and quality in this region. Therefore, a study was conducted during the 2014–2015 (Year 1) and 2015–2016 (Year 2) seasons in Tennessee. Twenty-three varieties were planted in a randomized complete block design with four replications across both years …
Diversification Through ‘Fun In The Farm’: Analyzing Structural Factors Affecting Agritourism In Tennessee, Aditya R. Khanal, Ummey Honey, Omobolaji Omobitan
Diversification Through ‘Fun In The Farm’: Analyzing Structural Factors Affecting Agritourism In Tennessee, Aditya R. Khanal, Ummey Honey, Omobolaji Omobitan
Agricultural and Environmental Sciences Faculty Research
With recent development, agritourism – visiting a working agricultural setting such as farm and ranch for leisure, recreation, or educational purposes – is gaining popularity in the US. Agritourism can be considered as a viable alternative agricultural enterprise to enhance income and sustainability of farms. This study maps agritourism locations and analyzes the structural factors affecting agritourism location and establishments. Using zip-code level location information, county-level data from Tennessee and spatial regression models, we found significant effects of rural factors, demographics, and educational-, economic-, and cultural- capital factors and their interactions on the location and establishment of agritourism farms. The …
Safety Of Food And Water Supplies In The Landscape Of Changing Climate, Aliyar Cyrus Fouladkhah, Brian Thompson, Janey Smith Camp
Safety Of Food And Water Supplies In The Landscape Of Changing Climate, Aliyar Cyrus Fouladkhah, Brian Thompson, Janey Smith Camp
Agricultural and Environmental Sciences Faculty Research
In response to evolving environmental, production, and processing conditions, microbial communities have tremendous abilities to move toward increased diversity and fitness by various pathways such as vertical and horizontal gene transfer mechanisms, biofilm formation, and quorum sensing
Unmanned Aircraft System (Uas) Technology And Applications In Agriculture, Samuel C. Hassler, Fulya Baysal-Gurel
Unmanned Aircraft System (Uas) Technology And Applications In Agriculture, Samuel C. Hassler, Fulya Baysal-Gurel
Agricultural and Environmental Sciences Faculty Research
Numerous sensors have been developed over time for precision agriculture; though, only recently have these sensors been incorporated into the new realm of unmanned aircraft systems (UAS). This UAS technology has allowed for a more integrated and optimized approach to various farming tasks such as field mapping, plant stress detection, biomass estimation, weed management, inventory counting, and chemical spraying, among others. These systems can be highly specialized depending on the particular goals of the researcher or farmer, yet many aspects of UAS are similar. All systems require an underlying platform—or unmanned aerial vehicle (UAV)—and one or more peripherals and sensing …
Container Size And Shade Duration Affect Growth Of Flowering Dogwood, Anthony L. Witcher, Fulya Baysal-Gurel, Eugene K. Blythe, Donna C. Fare
Container Size And Shade Duration Affect Growth Of Flowering Dogwood, Anthony L. Witcher, Fulya Baysal-Gurel, Eugene K. Blythe, Donna C. Fare
Agricultural and Environmental Sciences Faculty Research
Flowering dogwood (Cornus florida) is a valuable nursery product typically produced as a field-grown crop. Container-grown flowering dogwood can grow much faster than field-grown plants, thus shortening the production cycle, yet unacceptable crop loss and reduced quality continue to be major issues with container-grown plants. The objective of this research was to evaluate the effects of container size and shade duration on growth of flowering dogwood cultivars Cherokee Brave™ and Cherokee Princess from bare-root liners. In 2015, bare-root liners were transplanted to 23-L (no. 7) containers and placed under shade for 0 months (full sun), 2 months (sun4/shade2), …
Development Of Salmonellosis As Affected By Bioactive Food Compounds, Ajay Kumar, Abimbola Allison, Monica Henry, Anita Scales, Aliyar Cyrus Fouladkhah
Development Of Salmonellosis As Affected By Bioactive Food Compounds, Ajay Kumar, Abimbola Allison, Monica Henry, Anita Scales, Aliyar Cyrus Fouladkhah
Agricultural and Environmental Sciences Faculty Research
Infections caused by Salmonella serovars are the leading cause of foodborne hospitalizations and deaths in Americans, extensively prevalent worldwide, and pose a considerable financial burden on public health infrastructure and private manufacturing. While a comprehensive review is lacking for delineating the role of dietary components on prevention of Salmonellosis, evidence for the role of diet for preventing the infection and management of Salmonellosis symptoms is increasing. The current study is an evaluation of preclinical and clinical studies and their underlying mechanisms to elaborate the efficacy of bioactive dietary components for augmenting the prevention of Salmonella infection. Studies investigating dietary components …
Conservation Risk Of Batrachochytrium Salamandrivorans To Endemic Lungless Salamanders, Edward Davis Carter, Debra L. Miller, Anna C. Peterson, William B. Sutton, Joseph Patrick W. Cusaac, Jennifer A. Spatz, Louise Rollins-Smith, Laura Reinert, Markese Bohanon, Lori A. Williams, Andrea Upchurch, Matthew J. Gray
Conservation Risk Of Batrachochytrium Salamandrivorans To Endemic Lungless Salamanders, Edward Davis Carter, Debra L. Miller, Anna C. Peterson, William B. Sutton, Joseph Patrick W. Cusaac, Jennifer A. Spatz, Louise Rollins-Smith, Laura Reinert, Markese Bohanon, Lori A. Williams, Andrea Upchurch, Matthew J. Gray
Agricultural and Environmental Sciences Faculty Research
The emerging fungal pathogen, Batrachochytrium salamandrivorans (Bsal), is a significant conservation threat to salamander biodiversity in Europe, although its potential to affect North American species is poorly understood. We tested the susceptibility of two genera (Eurycea and Pseudotriton) and three populations of lungless salamanders (Plethodontidae) to Bsal. All species became infected with Bsal and two (Pseudotriton ruber and Eurycea wilderae) developed chytridiomycosis. We also documented that susceptibility of E. wilderae differed among populations. Regardless of susceptibility, all species reduced feeding when exposed to Bsal at the highest zoospore dose, and P. ruber and one population of E. wilderae used cover …
Effects Of Creep Feeding And Its Interactions With Other Factors On The Performance Of Meat Goat Kids And Dams When Managed On Pasture, Emily G. Hayes, Raquel V. Lourençon, Richard Browning Jr.
Effects Of Creep Feeding And Its Interactions With Other Factors On The Performance Of Meat Goat Kids And Dams When Managed On Pasture, Emily G. Hayes, Raquel V. Lourençon, Richard Browning Jr.
Agricultural and Environmental Sciences Faculty Research
Creep feeding and its possible interactions with other influential factors (genetics, litter type, and sex) for weaning traits were studied in meat goat kids and their dams. Kids across 3 yr were creep fed (254 kids; 5 pens) or not creep fed (255 kids; 5 pens) from 30 to 90 d of age. Creep-fed kids had higher (P ≤ 0.05) preweaning average daily weight gain and weaning weights (113.1 ± 13.0 g/d; 15.0 ± 0.8 kg) than kids not creep fed (99.8 ± 13.1 g/d; 14.0 ± 0.8 kg). However, financial returns were not higher (P > 0.05) for …
Detection Of Novel Allelic Variations In Soybean Mutant Population Using Tilling By Sequencing, Reneth Millas, Mary Espina, C. M. Sabbir Ahmed, Angelina Bernardini, Ekundayo Adeleke, Zeinab Yadegari, Korsi Dumenyo, Ali Taheri
Detection Of Novel Allelic Variations In Soybean Mutant Population Using Tilling By Sequencing, Reneth Millas, Mary Espina, C. M. Sabbir Ahmed, Angelina Bernardini, Ekundayo Adeleke, Zeinab Yadegari, Korsi Dumenyo, Ali Taheri
Agricultural and Environmental Sciences Faculty Research
One of the most important tools in genetic improvement is mutagenesis, which is a useful tool to induce genetic and phenotypic variation for trait improvement and discovery of novel genes. JTN-5203 (MG V) mutant population was generated using an induced ethyl methane sulfonate (EMS) mutagenesis and was used for detection of induced mutations in FAD2-1A and FAD2-1B genes using reverse genetics approach. Optimum concentration of EMS was used to treat 15,000 bulk JTN-5203 seeds producing 1,820 M2 population. DNA was extracted, normalized, and pooled from these individuals. Specific primers were designed from FAD2-1A and FAD2-1B genes that are involved in …
Overexpression Of Pear (Pyrus Pyrifolia) Cad2 In Tomato Affects Lignin Content, Mingtong Li, Chenxia Cheng, Xinfu Zhang, Suping Zhou, Lixia Li, Shaolan Yang
Overexpression Of Pear (Pyrus Pyrifolia) Cad2 In Tomato Affects Lignin Content, Mingtong Li, Chenxia Cheng, Xinfu Zhang, Suping Zhou, Lixia Li, Shaolan Yang
Agricultural and Environmental Sciences Faculty Research
PpCAD2 was originally isolated from the ‘Wangkumbae’ pear (Pyrus pyrifolia Nakai), and it encodes for cinnamyl alcohol dehydrogenase (CAD), which is a key enzyme in the lignin biosynthesis pathway. In order to verify the function of PpCAD2, transgenic tomato (Solanum lycopersicum) ‘Micro-Tom’ plants were generated using over-expression constructs via the agrobacterium-mediated transformation method. The results showed that the PpCAD2 over-expression transgenic tomato plant had a strong growth vigor. Furthermore, these PpCAD2 over-expression transgenic tomato plants contained a higher lignin content and CAD enzymatic activity in the stem, leaf and fruit pericarp tissues, and formed a greater number of vessel elements …
Growth, Flowering, And Powdery Mildew-Related Responses Of Witchhazels In Tennessee, Lisa W. Alexander, Anthony L. Witcher, Fulya Baysal-Gurel
Growth, Flowering, And Powdery Mildew-Related Responses Of Witchhazels In Tennessee, Lisa W. Alexander, Anthony L. Witcher, Fulya Baysal-Gurel
Agricultural and Environmental Sciences Faculty Research
Witchhazel (Hamamelis sp.) cultivars are now available in an array of forms and flower colors, including several native, pollinator-friendly cultivars. However, little is known about response of witchhazel cultivars to powdery mildew (Podosphaera biuncinata) or the growth and flowering characteristics of witchhazel cultivars in a nursery field production setting. To provide growth, flowering, and disease incidence data to nursery growers, a cultivar trial including 23 cultivars of witchhazel representing five species was planted Apr. 2016 in McMinnville, TN. Plant growth, flowering density, length of bloom, and foliar disease incidence were evaluated over three growing seasons between May …
Cross-Species Genome-Wide Analysis Reveals Molecular And Functional Diversity Of The Unconventional Interferon-Ω Subtype, Lauren E. Shields, Jordan Jennings, Qinfang Liu, Jinhwa Lee, Wenjun Ma, Frank Blecha, Laura C. Miller, Yongming Sang
Cross-Species Genome-Wide Analysis Reveals Molecular And Functional Diversity Of The Unconventional Interferon-Ω Subtype, Lauren E. Shields, Jordan Jennings, Qinfang Liu, Jinhwa Lee, Wenjun Ma, Frank Blecha, Laura C. Miller, Yongming Sang
Agricultural and Environmental Sciences Faculty Research
Innate immune interferons (IFNs), particularly type I IFNs, are primary mediators regulating animal antiviral, antitumor, and cell-proliferative activity. These antiviral cytokines have evolved remarkable molecular and functional diversity to confront ever-evolving viral threats and physiological regulation. We have annotated IFN gene families across 110 animal genomes, and showed that IFN genes, after originating in jawed fishes, had several significant evolutionary surges in vertebrate species of amphibians, bats and ungulates, particularly pigs and cattle. For example, pigs have the largest but still expanding type I IFN family consisting of nearly 60 IFN-coding genes that encode seven IFN subtypes including multigene subtypes …
Assessing Root System Architecture Of Wheat Seedlings Using A High-Throughput Root Phenotyping System, Ekundayo Adeleke, Reneth Millas, Waymon Mcneal, Justin Faris, Ali Taheri
Assessing Root System Architecture Of Wheat Seedlings Using A High-Throughput Root Phenotyping System, Ekundayo Adeleke, Reneth Millas, Waymon Mcneal, Justin Faris, Ali Taheri
Agricultural and Environmental Sciences Faculty Research
Background and aims Root system architecture is a vital part of the plant that has been shown to vary between species and within species based on response to genotypic and/or environmental influences. The root traits of wheat seedlings is critical for the establishment and evidently linked to plant height and seed yield. However, plant breeders have not efficiently developed the role of RSA in wheat selection due to the difficulty of studying root traits.
Methods We set up a root phenotyping platform to characterize RSA in 34 wheat accessions. The phenotyping pipeline consists of the germination paper-based moisture replacement system, …
Porcine Interferon Complex And Co-Evolution With Increasing Viral Pressure After Domestication, Jordan Jennings, Yongming Sang
Porcine Interferon Complex And Co-Evolution With Increasing Viral Pressure After Domestication, Jordan Jennings, Yongming Sang
Agricultural and Environmental Sciences Faculty Research
Consisting of nearly 60 functional genes, porcine interferon (IFN)-complex represents an evolutionary surge of IFN evolution in domestic ungulate species. To compare with humans and mice, each of these species contains about 20 IFN functional genes, which are better characterized using the conventional IFN-α/β subtypes as examples. Porcine IFN-complex thus represents an optimal model for studying IFN evolution that resulted from increasing viral pressure during domestication and industrialization. We hypothesize and justify that porcine IFN-complex may extend its functionality in antiviral and immunomodulatory activity due to its superior molecular diversity. Furthermore, these unconventional IFNs could even confer some functional and …
The Gene Encoding Nad-Dependent Epimerase/Dehydratase, Wcag, Affects Cell Surface Properties, Virulence, And Extracellular Enzyme Production In The Soft Rot Phytopathogen, Pectobacterium Carotovorum, Rabiul Islam, Shyretha Brown, Ali Taheri, C. Korsi Dumenyo
The Gene Encoding Nad-Dependent Epimerase/Dehydratase, Wcag, Affects Cell Surface Properties, Virulence, And Extracellular Enzyme Production In The Soft Rot Phytopathogen, Pectobacterium Carotovorum, Rabiul Islam, Shyretha Brown, Ali Taheri, C. Korsi Dumenyo
Agricultural and Environmental Sciences Faculty Research
Pectobacterium carotovorum is a gram-negative bacterium that, together with other soft rot Enterobacteriaceae causes soft rot disease in vegetables, fruits, and ornamental plants through the action of exoproteins including plant cell wall-degrading enzymes (PCWDEs). Although pathogenicity in these bacteria is complex, virulence levels are proportional to the levels of plant cell wall-degrading exoenzymes (PCWDEs) secreted. Two low enzyme-producing transposon Tn5 mutants were isolated, and compared to their parent KD100, the mutants were less virulent on celery petioles and carrot disks. The inactivated gene responsible for the reduced virulence phenotype in both mutants was identified as wcaG. The gene, wcaG (previously …
The Cation Exchange Behavior Of Tylosin In Loess-Derived Soil, Jaime J. Call, Michael E. Essington, Sudipta Rakshit
The Cation Exchange Behavior Of Tylosin In Loess-Derived Soil, Jaime J. Call, Michael E. Essington, Sudipta Rakshit
Agricultural and Environmental Sciences Faculty Research
Tylosin (Tyl) is a veterinary antibiotic commonly used in swine and poultry production. Due to metabolic inefficiencies, it enters the environment through manure applications. Ion exchange is an important retention mechanism for Tyl, particularly for smectite clay. The objectives of this study are to characterize the exchange interactions of Tyl with common soil cations in subsoil horizons that contain smectite and to investigate the interactions using in situ Fourier transform infrared (FTIR) spectroscopy. Adsorbed Tyl in pH neutral, smectitic subsoil horizons is divided into exchangeable and nonexchangeable forms. The percentage of adsorbed Tyl that is exchangeable varies from 36% to …