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Thermal Shift-Induced Gene Expression And Regulation In Maize (Zea Mays L), Christopher Leon Baszczynski
Thermal Shift-Induced Gene Expression And Regulation In Maize (Zea Mays L), Christopher Leon Baszczynski
Digitized Theses
Rapid elevation in the incubation temperature of maize seedlings leads to the new and(or) enhanced synthesis of a group of heat shock polypeptides (HSPs). The response is: (a) rapid (HSP synthesis is detectable within 15 minutes following thermal shifts); (b) reversible (the "pre-shift" pattern of polypeptide synthesis is re-established when seedlings are returned to the control temperature); and (c) transitory (maintenance of seedlings at the elevated temperature leads to a gradual reduction of HSP production and the establishment of a new pattern of polypeptide synthesis). The synthesis of an apparently identical set of HSPs is noted in maize plumules, mesocotyls, …
Factors That Influence The Size Of The Repair Patch In The Dna Of Cultured Human Cells, John Peng Th'ng
Factors That Influence The Size Of The Repair Patch In The Dna Of Cultured Human Cells, John Peng Th'ng
Digitized Theses
The buoyant density shift method was employed in the study of the enzymology and its regulation of DNA repair in cultured human cells. Measurements of the size of the DNA repair patch after treatments with UV-light or methylating agents revealed that about 30 nucleotides are replaced at each damage site, and that the extent of replacement is independent of the DNA-damaging agents employed.;In the examination of the role of poly(ADP-ribosyl)ation in DNA repair, the size of the repair patch was not increased by the presence of 3-aminobenzamide (3-AB), an inhibitor of poly(ADP-ribose) polymerase, as had been predicted by other investigators.;To …
Mating Type, Vitamin E And Morphogenesis In Ustilago Violacea And Other Smut Fungi, Alan James Castle
Mating Type, Vitamin E And Morphogenesis In Ustilago Violacea And Other Smut Fungi, Alan James Castle
Digitized Theses
The anther smut fungus, Ustilago violacea, grows either saprophytically (yeastlike cells) or as a parasite of the Caryophyllaceae (mycelial cells). Factors affecting two types of development controlled by the mating type gene, sporulation and myceliation, were examined.;Sporulation. Freshly isolated diploid cells, heterozygous for the mating type alleles, a(,1) and a(,2), develop into sexual phase precursor cells (SPP) on complete medium at temperatures below 20(DEGREES)C. The a(,1)/a(,2) diploid produces at least four different cell types, termed opaques, spontaneously at high frequencies (> 3 x 10('-3)). These types are: (1) neutral strains (op-N) which do not mate and still initiate sporulation but …