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A Novel Mistranslating Trna Model In Drosophila Melanogaster Has Diverse, Sexually Dimorphic Effects, Joshua R. Isaacson, Matthew D. Berg, Brendan Charles, Jessica Jagiello, Judit Villén, Christopher J. Brandl, Amanda J. Moehring
A Novel Mistranslating Trna Model In Drosophila Melanogaster Has Diverse, Sexually Dimorphic Effects, Joshua R. Isaacson, Matthew D. Berg, Brendan Charles, Jessica Jagiello, Judit Villén, Christopher J. Brandl, Amanda J. Moehring
Neuroscience Institute Publications
Transfer RNAs (tRNAs) are the adaptor molecules required for reading the genetic code and producing proteins. Transfer RNA variants can lead to genome-wide mistranslation, the misincorporation of amino acids not specified by the standard genetic code into nascent proteins. While genome sequencing has identified putative mistranslating transfer RNA variants in human populations, little is known regarding how mistranslation affects multicellular organisms. Here, we create a multicellular model of mistranslation by integrating a serine transfer RNA variant that mistranslates serine for proline (tRNAUGG,G26ASer) into the Drosophila melanogaster genome. We confirm mistranslation via mass spectrometry and find that tRNAUGG,G26ASer misincorporates serine for …