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Comparing Apples And Oranges (And Blueberries And Grapes): Fruit Type Affects Development And Cold-Susceptibility Of Immature Drosophila Suzukii, Yanira Jiménez Padilla, Laura V. Ferguson, Brent J. Sinclair
Comparing Apples And Oranges (And Blueberries And Grapes): Fruit Type Affects Development And Cold-Susceptibility Of Immature Drosophila Suzukii, Yanira Jiménez Padilla, Laura V. Ferguson, Brent J. Sinclair
Biology Publications
Drosophila suzukii is a cosmopolitan polyphagous pest on unripe soft-skinned fruits. We sought to determine 1) temperature treatments that could be used to kill immature D. suzukii in fruit or packaging, and 2) whether development on different fruits led to differences in cold tolerance of immature D. suzukii. We reared animals from egg on a banana-based laboratory diet and diets made of apple, blueberry, cherry, grape, orange, raspberry, or strawberry homogenate in agar, and measured development time, adult body size, and cold tolerance. Diet type had complex effects on development time; in particular, flies reared on apple- or blueberry-based …
Persistence Of Diet Effects On The Drosophila Suzukii Microbiota, Yanira Jiménez Padilla, Ebenezer Esan, Kevin D. Floate, Brent J. Sinclair
Persistence Of Diet Effects On The Drosophila Suzukii Microbiota, Yanira Jiménez Padilla, Ebenezer Esan, Kevin D. Floate, Brent J. Sinclair
Biology Publications
The insect commensal microbiota consists of prokaryotes and eukaryotes. The effect of diet and the persistence of the gut microbiota in Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) are not well-understood. We transferred subsets of a single population of D. suzukii to different fruit-based diets (blueberry, raspberry, and strawberry) for three generations and then returned them to a common, banana-based, laboratory diet. We used 16S (bacteria) and ITS (fungi) sequencing of female endosymbiont-free flies to identify the microbiota. We identified 2700 bacterial and 350 fungal OTUs; there was no correlation between the number of bacterial and fungal OTUs in a sample. Bacterial …
Dissection Of Anoplophora Glabripennis (Coleoptera: Cerambycidae) Larval Tissues For Physiological And Molecular Studies, Alex S. Torson, Lauren E. Des Marteaux, Susan Bowman, Meng Lei Zhang, Kevin Ong, Daniel Doucet, Brent J Sinclair, Amanda D. Roe
Dissection Of Anoplophora Glabripennis (Coleoptera: Cerambycidae) Larval Tissues For Physiological And Molecular Studies, Alex S. Torson, Lauren E. Des Marteaux, Susan Bowman, Meng Lei Zhang, Kevin Ong, Daniel Doucet, Brent J Sinclair, Amanda D. Roe
Biology Publications
Many biological processes are partitioned among organs and tissues, necessitating tissue-specific or organ-specific analysis (particularly for comparative -omics studies). Standardised techniques for tissue identification and dissection are therefore imperative for comparing among studies. Here we describe dissection protocols for isolating six key tissues/organs from larvae of the Asian longhorned beetle, Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae): the supraoesophageal ganglion, posterior midgut, hindgut, Malpighian tubules, fat body, and thoracic muscle. We also describe how to extract haemolymph and preserve whole larvae for measurements such as protein, lipid, and carbohydrate content. We include dissection protocols for both fresh-killed and previously frozen specimens. Although …
Thermal Variability And Plasticity Drive The Outcome Of A Host-Pathogen Interaction, Laura V. Ferguson, Brent J. Sinclair
Thermal Variability And Plasticity Drive The Outcome Of A Host-Pathogen Interaction, Laura V. Ferguson, Brent J. Sinclair
Biology Publications
Variable, changing, climates may affect each participant in a biotic interaction differently. We explored the effects of temperature and plasticity on the outcome of a host-pathogen interaction to try to predict the outcomes of infection under fluctuating temperatures. We infected Gryllus veletis crickets with the entomopathogenic fungus Metarhizium brunneum under constant (6 °C, 12 °C, 18 °C or 25 °C) or fluctuating temperatures (6 °C to 18 °C or 6 °C to 25 °C). We also acclimated crickets and fungi to constant or fluctuating conditions. Crickets acclimated to fluctuating conditions survived best under constant conditions if paired with warm-acclimated fungus. …