Digital Commons Network^™

In Situ Ion Exchange In A Micro-Porous Transition Metal Silicate Framework, Jason M. Lively

Masters Theses & Specialist Projects

Ion selectivity of minerals has traditionally been utilized in industry as a catalyst, metal separation, and environmental reclamation/sequestration tool. There is an increased interest in understanding ion selectivity mechanisms of micro-porous minerals and mineral-like structures and how they can be applied in various industries: environmental and, potentially, pharmaceutical. This study seeks to understand the ion exchange mechanisms in micro-porous zirconosilicates using time-resolved Raman spectroscopy and X-ray diffraction. The thesis material was exchanged with H+, Na+, K+, and Cs+ in order to better understand structural changes as well as the influence of the H+-bonding during the exchange process. It is hypothesized …

Dispersion Of Ammonia From Concentrated Animal Feeding Operations, Austin D. Wardall

Masters Theses & Specialist Projects

The purpose of this research is to investigate the dispersion of ammonia (NH3) from three Concentrated Animal Feeding Operations (CAFOs) in western Kentucky, as well as to investigate the Weather Research and Forecasting – Chemistry (WRF-Chem) model’s sensitivity response to initial NH3 concentrations under both wet conditions (significant precipitation) and dry conditions (no precipitation). As expected, pollutant concentrations generally were significantly higher near their points of origin and generally declined away from the sources. Contrary to expectations, ammonia tended to rise through the planetary boundary layer (PBL) regardless of atmospheric conditions. Results showed modeled NH3 pollution levels at the surface …

The Investigation Of The Electrical Control Of Hemimicelles And Admicelles On Gold For Analyte Preconcentration, Dheyaa Hussein Al-Karawi

Masters Theses & Specialist Projects

Hemimicelles and admicelles are well-investigated wonders in modern science; they are surfactant monolayers and surface adsorbed micelles, respectively. Capacitance measurements for monitoring the formation of dodecyl sulfate (DS) surfactant monolayer on positively charged gold substrates (planar gold) and the adsorbance of 2-naphthol onto DS surfactant monolayer were performed. The investigation of the electrical control of DS at various concentrations (4, 6, 16, and 32 mM) below and above the critical micelle concentration (CMC= 8 mM) on gold surfaces for analyte preconcentration, prior to chromatographic analysis, is presented. Charged ionic surfactants, such as DS, drawn to a surface of opposite charge …

Feasibility Of The Use Of Neutron Activation Analysis Techniques In An Underwater Environment, Michael D. Chick

Masters Theses & Specialist Projects

Elements when bombarded with neutrons emit a gamma ray that is characteristic of the isotope that underwent a neutron induced nuclear reaction; this is known as neutron activation. The characteristic gamma energy of an isotope can then be detected and recorded. One can then analyze the gamma energies captured and determine the elemental makeup of the sample. This form of analysis can be used in an underwater environment making it potentially a valuable tool for agencies tasked with maritime security of ports and waterways, or clean-up operations. This thesis will focus on the feasibility of neutron interrogation using pulsed fast/thermal …

The Effects Of Sulfuric Acid Deposition On The Growth And Development Of Pond Breeding Salamanders In The Genus Ambystoma, Kenneth J. Anderson

Masters Theses & Specialist Projects

In terrestrial habitats with a history of mining activity and previous or ongoing reclamation efforts, understanding the effects of acidification on the ecology of amphibians is an important part of the restoration process and the conservation of local amphibian populations. Pond-breeding amphibians spend much of their post metamorphic life history in direct contact with the soil in upland habitat adjacent to aquatic breeding sites. I reared recently metamorphosed marbled salamanders (Ambystoma opacum) to evaluate the role of soil acidity on determinants of fitness such as growth and survival. My results indicate that a substrate of pH 4 was lethal to …

Mabic: Mobile Application Builder For Interactive Communication, Huy Manh Nguyen

Masters Theses & Specialist Projects

Nowadays, the web services and mobile technology advance to a whole new level. These technologies make the modern communication faster and more convenient than the traditional way. People can also easily share data, picture, image and video instantly. It also saves time and money. For example: sending an email or text message is cheaper and faster than a letter. Interactive communication allows the instant exchange of feedback and enables two-way communication between people and people, or people and computer. It increases the engagement of sender and receiver in communication.

Although many systems such as REDCap and Taverna are built for …

Metal-Organic Hybrid Nanocomposites For Energy Harvesting Applications, Thulitha Madawa Abeywickrama

Masters Theses & Specialist Projects

Various synthetic methods have been developed to produce metal nanostructures including copper and iron nanostructures. Modification of nanoparticle surface to enhance their characteristic properties through surface functionalization with organic ligands ranging from small molecules to polymeric materials including organic semiconducting polymers is a key interest in nanoscience. However, most of the synthetic methods developed in the past depend widely on non-aqueous solvents, toxic reducing agents, and high temperature and high-pressure conditions. Therefore, to produce metal nanostructures and their nanocomposites with a simpler and greener method is indeed necessary and desirable for their nano-scale applications. Hence the objective of this thesis …

Development Of Dihydrochalcone Functionalized Gold Nanoparticles For Augmented Antineoplastic Activity, Jason N. Payne

Masters Theses & Specialist Projects

Phloridzin, an antidiabetic and antineoplastic agent usually found in fruit trees, is a dihydrochalcone constituent that has a clinical/pharmaceutical significance as a sodiumglucose linked transport 2 (SGLT2) inhibitor. Phloridzin never experienced widespread clinical usage in the pharmaceutical market due to its side effects and poor bioavailability when compared to other antidiabetic therapeutics. The poor bioavailability is primarily attributed to the degradation of the glycosidic bond of the phloridzin, resulting in the formation of phloretin, the aglycone of phloridzin and glucose. While phloretin displays a reduced capacity of SGLT2 inhibition, this nutraceutical shows enhanced antineoplastic activity in comparison to phloridzin. Gold …

Visible-Light Generation Of High-Valent Metal-Oxo Intermediates And A Biomimetic Oxidation Catalyzed By Manganese Porphyrins With Iodobenzene Diacetate, Ka Wai Kwong

Masters Theses & Specialist Projects

High-valent iron-oxo intermediates play central roles as active oxidants in enzymatic and synthetic catalytic oxidations. Many transition metal catalysts are designed for biomimetic studies of the predominant oxidation catalysts in Nature, the cytochrome P450 enzymes.

In this work, a new photochemical method to generate high-valent iron-oxo porphyrin models was discovered. As controlled by the electronic nature of porphyrin ligands, iron(IV)-oxo porphyrin radical cations (Compound I model) and iron(IV)-oxo porphyrin derivatives (Compound II model) were produced. These observations indicate that the photochemical reactions involve a heterolytic cleavage of O-Br in precursors to give a putative iron(V)-oxo intermediate, which might relax to …

Multiplexed Detection Of Double-Stranded Pathogenic Dna With Engineered Zinc Finger Proteins, Juhwa Kim

Masters Theses & Specialist Projects

The development of a new technology for the detection of doublestranded (ds) DNA enables multiple biomedical applications including identifying multiple pathogens simultaneously. We previously employed colorimetric SEquence-Enabled Reassembly with TEM-1 β-lacatamase (SEER-LAC) to detect specific bacterial DNA sequence. SEER-Lac consists of the two inactive β-lactamase fragments which of each attached to a zinc finger protein (ZFP) would reassemble into an active full-length enzyme upon ZFPs binding to its target DNA. Here, we engineered two pairs of ZFPs which of each recognizes shiga toxin in E. coli O157 and staphylococcal enterotoxin B in Staphylococus Aureus, respectively. Biotin was simply conjugated to …

Evaluating The Influences Of Karst Hydrogeology On Freshwater Harmful Algal Blooms In Kentucky Lakes, Robert T. Schaefer

Masters Theses & Specialist Projects

A problem exists in Nolin River Lake and Rough River Lake in Kentucky, due to the increasing prevalence of cyanobacterial-based harmful algal blooms (CyanoHABs) and the threats they pose to local communities. These lakes were developed as artificial reservoirs from embankment. Further complicating the issue, the lakes are located within a heavily karstified region and there exists no plan or method currently for monitoring or managing CyanoHABs in a karst region with regard to groundwater inputs to the lake systems or their tributaries. A mixture of techniques and analysis methods was used to determine the best way to monitor and …

Nullification Of Torus Knots And Links, Zachary S. Bettersworth

Masters Theses & Specialist Projects

Knot nullification is an unknotting operation performed on knots and links that can be used to model DNA recombination moves of circular DNA molecules in the laboratory. Thus nullification is a biologically relevant operation that should be studied.

Nullification moves can be naturally grouped into two classes: coherent nullification, which preserves the orientation of the knot, and incoherent nullification, which changes the orientation of the knot. We define the coherent (incoherent) nullification number of a knot or link as the minimal number of coherent (incoherent) nullification moves needed to unknot any knot or link. This thesis concentrates on the study …

Devonian Hydrocarbon Production In South Central Kentucky: A Gis Study, Andrew David Reeder

Masters Theses & Specialist Projects

Historically, Devonian Corniferous hydrocarbon production in south-central Kentucky has been a major contributor to the total hydrocarbon resources within Butler, Edmonson, and Warren counties. The Corniferous adds to the already large hydrocarbon totals produced from the Illinois Basin; however, this hydrocarbon contribution does not come without major pitfalls and inconsistencies. The south-central Kentucky Devonian Corniferous rocks comprise a diverse and complex hydrocarbon system made up of multiple migration pathways, trapping mechanisms, and seals. The multiple Corniferous units all derive production from the same source rock: the New Albany Shale.

A Geographic Information System (GIS) containing data on surface elevations, structure …

Linking In-Situ Data With Remote Sensing To Analyze Tropical Glacier Stability And Retreat In The Cordillera Blanca, Peru, Chandler H. Santos

Masters Theses & Specialist Projects

Glaciers are a major source of freshwater around the world, but they are melting at an increased rate due to atmospheric warming resulting from anthropogenic climate change. In addition to temperature increases, light-absorbing particulates on glaciers also are contributing to glacial melt. This research examines how black carbon, released into the air through partial combustion of biofuels, is affecting the surface albedo of glaciers. I also delineate possible sources of black carbon in the Cordillera Blanca region of Peru. Ground data were collected each year from 2011 to 2013 during the local dry season. Effective black carbon (eBC) values were …

Low-Cost Quartz Crystal Microbalance System Platform Designed For Chemical Nanoparticle, Danming Wei

Masters Theses & Specialist Projects

QCM sensor is a response to a kind of broad spectrum, high sensitivity, and simple structure, low-cost detection device, and particularly its quality as a type of gas sensor is widely used. With the successful oscillation in liquid phase, QCM sensor has been involved in the application analytical chemistry, surface chemistry, biochemistry and environmental monitoring side and many other scientific fields. With sensitive surface film as the sensitive element, AT-cut quartz crystal as energy transducer components by changes of the relationship between mass of surface film and frequency of QCM sensor transduces signals of mass or concentration into output frequency …

Using Zinc Finger Proteins As A Diagnostic Tool For The Detection Of A Cancer Biomarker, Anu Kini

Masters Theses & Specialist Projects

RASSF1A is a tumor suppressor gene which loses its function due to methylation of CpG islands on its promoter region. Detection of methylation leads to early diagnosis of cancer.

Zinc finger proteins are capable of detecting a specific DNA sequence and Methyl binding domain can bind to the methyl group on the CpG, using this idea mCpG SEER- Lac system makes use of a split protein, β-lactamase. Lac A attached to the ZFP and Lac B attached to the MBD protein. On binding to the DNA, the Lac A and Lac B come in close proximity with each other causing …

Synthetic Investigation On The Biomimetic Metal-Catalyzed Sulfoxidations And Photochemical Generation Of A Highly Reactive Ruthenium(V)-Oxo Porphyrin, Weilong Luo

Masters Theses & Specialist Projects

Catalytic oxidation plays a crucial role in current chemical and pharmaceutical industries which is also a leading technology for green chemical processes. In Nature, the ubiquitous cytochrome P450 enzymes can catalyze a wide variety of oxidation reactions with high efficiency and selectivity. Many transition metal catalysts are designed as the biomimetic model of cytochrome P450 enzymes. In this work, series of metalloporphyrins and metallocorroles have been successfully synthesized to investigate and develop catalytic selective oxidation of sulfides to sulfoxides.

Manganese(III) porphyrin complexes (2) and manganese(III) corrole complexes (6) with iodobenzene diacetate [PhI(OAc)2] as a mild oxygen source exhibited remarkable catalytic …

Karst Landscape Influence On The Planetary Boundary Layer Atmosphere, Zachary S. Sullivan

Masters Theses & Specialist Projects

Karst landscapes cover approximately 20% of the ice-free land area worldwide. The soluble nature of the bedrock within a karst landscape allows for the formation of caverns, joints, fissures, sinkholes, and underground streams, which affect the hydrological behavior of the region. Currently, the Noah Land-Surface Model (Noah- LSM), coupled with the Weather Research and Forecasting (WRF) model, does not provide a representation of the physical behavior of a karst terrain. Previous research has attempted to model karst behavior through soil moisture and land cover/land use changes to determine the influence this unique landscape may have on atmospheric phenomenon. This highlights …

Vertical Implementation Of Cloud For Education (V.I.C.E.), Travis S. Brummett

Masters Theses & Specialist Projects

There are several different implementations of open source cloud software that organizations can utilize when deploying their own private cloud. Some possible solutions are OpenNebula, Nimbus, and Eucalyptus. These are Infrastructure-as-a-Service (IaaS) cloud implementations that ultimately gives users virtual machines to undefined job types. A typical IaaS cloud is composed of a front-end cloud controller node, a cluster controller node for controlling compute nodes, a virtual machine image repository node, and many persistent storage nodes and compute nodes. These architectures are built for ease of scalability and availability.

Interestingly, the potential of such architectures could have in the educational field …

The Role Of Diagenesis In Reservoir Development Of The Big Clifty (Jackson) Sandstone In South-Central Kentucky, Kort H. Butler

Masters Theses & Specialist Projects

The Big Clifty is a Chesterian-age Mississippian sandstone member of the Golconda Formation that extends from south-central Kentucky into adjacent Illinois and Indiana. Asphaltic deposits and conventional petroleum plays are distributed along the Pennyrile Fault System and Plateau in the southeastern portion of the Illinois basin. In south-central Kentucky anomalous oil-saturation geometries are observed in cored sections of the Big Clifty from wells in Warren and Butler counties along the margin of the Pennyrile Plateau. Petrographic study of the cores has revealed several diagenetic processes that have contributed to, or are directly responsible for, the anomalous oil saturation geometries and …

Investigation Of Carbon Nanomaterials Embedded In A Cementitious Matrix, Clarissa A. Roe

Masters Theses & Specialist Projects

The objective of this thesis was to investigate whether the addition of carbon nanofibers had an effect on the splitting tensile strength of Hydro-Stone gypsum concrete. The carbon nanofibers used were single-walled carbon nanotubes (SWNT), buckminsterfullerene (C60), and graphene oxide (GO). Evidence of the nanofibers interacting with gypsum crystals in a connective manner was identified in both 1 mm thick concrete discs and concrete columns possessing a height of 2 in and a diameter of 1 in. Before imaging, the columns were subjected to a splitting tensile strength test. The results illustrate that while there is a general decrease in …

Apatite Helium Thermochronology Of The Blue Nile Canyon, Ethiopian Plateau, Nathaniel C. Blackburn

Masters Theses & Specialist Projects

The Ethiopian Plateau, situated in the northernmost part of the East African Rift System, has been uplifted and incised by the Blue Nile River, creating the 1.5 km-deep Blue Nile Canyon, the only extensive canyon within the Nile drainage basin. Much of this canyon incision is related to the Ethiopian Plateau uplift, which is due to a combined tectonic influence linked to the East African Rift System and the Afar Mantle Plumerelated upwelling since the Oligocene. The Blue Nile Canyon incision followed this plume-related upwelling, which resulted in extensive flood basalt emplacement around ~30 Ma. This study employs low-temperature apatite …

In The Face Of Anticipation: Decision Making Under Visible Uncertainty As Present In The Safest-With-Sight Problem, Bryan A. Knowles

Masters Theses & Specialist Projects

Pathfinding, as a process of selecting a fixed route, has long been studied in

Computer Science and Mathematics. Decision making, as a similar, but intrinsically different, process of determining a control policy, is much less studied. Here, I propose a problem that appears to be of the first class, which would suggest that it is easily solvable with a modern machine, but that would be too easy, it turns out. By allowing a pathfinding to anticipate and respond to information, without setting restrictions

on the \structure" of this anticipation, selecting the \best step" appears to be an intractable problem.

After …

The Influence Of Tropical Cyclones On Droughts And Warm Season Precipitation In Tennessee And Kentucky, Lamar S. Coats

Masters Theses & Specialist Projects

The Southeast United States during summer and fall is often affected by droughts and tropical cyclones. Both phenomena rank among the most expensive of natural hazards, although droughts are not as feared by the public as hurricanes. When a tropical cyclone causes a pendulum swing from drought to wet conditions, it is known as a “drought-busting tropical cyclone.” The majority of the research related to drought busting tropical cyclones investigates only the storms during their tropical cyclone phase, which covers the southeastern states that have boundaries adjacent to the Atlantic Ocean. An unanswered question from this literature is whether or …

Pedestrian Detection Using Basic Polyline: A Geometric Framework For Pedestrian Detection, Liang Gongbo

Masters Theses & Specialist Projects

Pedestrian detection has been an active research area for computer vision in recently years. It has many applications that could improve our lives, such as video surveillance security, auto-driving assistance systems, etc. The approaches of pedestrian detection could be roughly categorized into two categories, shape-based approaches and appearance-based approaches. In the literature, most of approaches are appearance-based. Shape-based approaches are usually integrated with an appearance-based approach to speed up a detection process.

In this thesis, I propose a shape-based pedestrian detection framework using the geometric features of human to detect pedestrians. This framework includes three main steps. Give a static …

An Adaptive Visual Learning Approach For Waterborne Disease Prevention In Rural West Africa, Jonathan Le Mar Oglesby

Masters Theses & Specialist Projects

Depleted water supplies, along with industrial and human waste, are driving a world water crisis that poses a growing risk to food markets, energy production, political stability, and human health (Global Water Security 2012). One obvious example of the this crisis is the more than 1 billion people who obtain their drinking water from contaminated sources (WHO/UNICEF 2006; Clasen et al. 2008). With a projected increase of 1.3 billion people between now and 2050, Africa will add more to the global population than any other world region (Haub and Kaneda 2013). For this research, visual learning design factors were incorporated …

Two Generalizations Of The Filippov Operation, Menevse Eryuzlu

Masters Theses & Specialist Projects

The purpose of this thesis is to generalize Filippov's operation, and to get more useful results. It includes two main parts: The C-Filippov operation for the finite and countable cases and the Filippov operation with different measures. In the first chapter, we give brief information about the importance of Filippov's operation, our goal and the ideas behind our generalizations. In the second chapter, we give some sufficient background notes. In the third chapter, we introduce the Filippov operation, explain how to calculate the Filippov of a function and give some sufficient properties of it. In the fourth chapter, we introduce …

Petrographic Controls On Weathering Of The Haney Limestone, Steven M. Devine

Masters Theses & Specialist Projects

Although karst processes in south central Kentucky have been studied extensively, the Haney Limestone Member of the Golconda Formation has not been studied in detail in contrast to limestones stratigraphically below it that are thicker. In addition, the relationship between petrographic features of the Haney Limestone and the formation of caves and karst features has not been studied extensively compared to lithographic, petrographic, or structural variables

Petrographic data were collected using core and surface exposures across the study area of south central Kentucky from northern Logan and Warren counties up toward the Rough Creek Graben region, and stratigraphic columns were …

Synthesis And Characterization Of Organic-Inorganic Hybrid Materials For Thermoelectric Devices, Paige M. Huzyak

Masters Theses & Specialist Projects

The development of organic-inorganic hybrid materials is of great interest in thermoelectrics for its potential to combine the desirable characteristics of both classes of materials. Thermoelectric materials must combine low thermal conductivity with high electrical conductivity, but in most materials, thermal and electrical conductivity are closely related and positively correlated. By combining the low thermal conductivity, flexibility, facile processing, and low cost of organic components with the high electrical conductivity and stability of inorganic components, materials with beneficial thermoelectric properties may be realized.

Here, we describe the synthesis and characterization of anthracene-containing organic-inorganic hybrid materials for thermoelectric purposes. Specifically, POSS-ANT …

Binding Of Oxaliplatin And Its Analogs With Dna Nucleotides At Variable Ph And Concentration Levels, Rippa Sehgal

Masters Theses & Specialist Projects

Oxaliplatin is one of the three FDA-approved platinum anticancer drugs and considered a third generation drug, discovered after the first generation drug cisplatin and second generation drug carboplatin. It is known to react with proteins and DNA nucleotides in the body. Reaction with DNA occurs primarily at guanosine residues and secondarily at adenine residues for oxaliplatin and other platinum drugs. We have previously studied oxaliplatin and an analog with additional steric hindrance in the amine ligand and found that the analog had different reactivity with methionine. Now, we have prepared oxaliplatin and its three analogs Pt(Me2dach)(ox), Pt(en)(ox) and Pt(Me4en)(ox) and …