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Differentially Expressed Genes In The Mutualistic Neotyphodium Coenophialum-Tall Fescue Interaction, Linda Joy Johnson
Differentially Expressed Genes In The Mutualistic Neotyphodium Coenophialum-Tall Fescue Interaction, Linda Joy Johnson
Graduate Theses, Dissertations, and Problem Reports
Tall fescue (Lolium arundinaceum), an agronomically important forage grass, is typically associated with a mutualistic asexual fungus Neotyphodium coenophialum. Endophytic fungal growth is confined to the intercellular spaces of the plant and induces no symptoms. The endophyte partner enhances its host's fitness by improving nutrient acquisition as well as by providing protection from various abiotic and biotic stresses. However, grass-endophyte partnerships are detrimental to grazing livestock, due to the production of an array of fungal alkaloids. To investigate this interaction at the molecular level, suppressive subtractive hybridization was used to identify differentially expressed genes from the symbiosis. In total, twenty-nine …
Vanadate-Induced Cell Cycle Regulation And Its Signal Transduction Pathway, Zhuo Zhang
Vanadate-Induced Cell Cycle Regulation And Its Signal Transduction Pathway, Zhuo Zhang
Graduate Theses, Dissertations, and Problem Reports
Vanadate is potent toxic and carcinogenic agent. The mechanisms of its toxic and carcinogenic actions are still under investigation. Cell cycle arrest is an important mechanism in maintaining genomic stability and integrity in response to environmental stress. The present study investigated the possible mechanisms involved in vanadate-induced cell cycle arrest and its signal transduction pathway. The results show that vanadate caused G2/M phase arrest A549 cells. p21 and cdc25C affected on cyclin B/cdc2 complex, resulting in the cell cycle arrest at G2/M phase. p38 and extracellular signal-regulated protein kinase (ERK) were involved in the regulation of these cell cycle regulatory …
Determining The Effects Of Phosphorylation On Afap-110 Function, Lidia Nikolayevna Cherezova
Determining The Effects Of Phosphorylation On Afap-110 Function, Lidia Nikolayevna Cherezova
Graduate Theses, Dissertations, and Problem Reports
As there are over twenty potential PKC phosphorylation sites in AFAP-110 structure, deletional mutagenesis was employed to determine which regions of AFAP-110 are most likely to contain crucial sites for PKC phosphorylation. Our data indicate that functional PKC phosphorylation sites must be localized to the amino acid stretch 266--294 within STK region of AFAP-110. There are four PKC candidate phosphorylation sites in this region; they are being substituted by alanine residues in order to determine their significance in serine/threonine phosphorylation regulation of AFAP-110 function.;Analysis of AFAP-110 amino acid sequence has also revealed that AFAP-110 contains twelve potential 14-3-3 binding sequences. …
Triplex Formation As Monitored By Epr Spectroscopy And Molecular Dynamics Studies Of Spin -Probe -Labeled Dnas, Eva Darian
Graduate Theses, Dissertations, and Problem Reports
Molecular modeling has proven to be a powerful tool for studying structure and dynamics of biologically important molecules. Since the advent of nitroxide based spin-probes the electron paramagnetic resonance (EPR) study of spin-labeled macromolecules has been able to provide insight into structural and dynamics properties of DNAs, proteins, and related systems. Spin labels have been extensively used to study the dynamics of oligonucleotides. An example of this is 5-membered ring nitroxide 5-(2,2,5,5-tetramethyl-3-ethynylpyrrolidine-1-oxyl)-uridine, which has been previously used in our laboratory to monitor triplex formation. Because of the difficult synthetic steps involved in the synthesis of this particular probe a new …
Interaction Between Positive And Negative Effectors Of Embryonic And Postnatal Muscle Growth In The Chicken And The Mouse, Scott Allen Gahr
Interaction Between Positive And Negative Effectors Of Embryonic And Postnatal Muscle Growth In The Chicken And The Mouse, Scott Allen Gahr
Graduate Theses, Dissertations, and Problem Reports
Myostatin, belonging to the TGF-beta family, negatively regulates skeletal muscle growth. The current study was conducted to assess the efficacy of in ovo administration of exogenous myostatin antagonist (MA) to enhance skeletal muscle growth and improve feed efficiency of broilers.;Growth hormone secretion is under the control of a pair of hypothalamic factors, growth hormone releasing hormone and somatostatin. The growth hormone secretagogue receptor (GHSR) and its endogenous ligand represent a novel third mechanism regulating the release of growth hormone. Early chicken embryonic development, prior to day 14, has been proposed to be independent of GH. However, recent evidence shows that …